Transovarial Transmission and Dengue Virus Serotypes in Aedes Aegypti in Kupang

Dengue is an endemic disease in almost all provinces in Indonesia, including NTT pro-vince and Kupang City. DHF prevention and control efforts need to be accompanied by understanding of the epidemiological and entomological aspects. This study proves the presence of transovarial transmission and serotypes of Dengue virus in Ae. aegypti in areas with different endemics in Kupang City. The study was conducted in endemic, sporadic and Dengue-free villages. Samples from each location were 30 head squash Ae. aegypti females for examination of Dengue virus by IHC methods and 10 Ae. aegypti females for serotypes check of Dengue virus with RTPCR. Data presented in tables and images then analyzed descriptively. This study finds that transovarial infection rates 9.2% with serotype Dengue virus-1. As many 97.5% of respondents have heard about Dengue by most cadres resources (24.5%) and health workers (24%). Transovarial infection is found in endemic, sporadic and free areas so that the Dengue vector control needs to be done in the three regions with different endemicity

Kondisi Sarana Air Bersih, Perilaku Hidup Bersih dan Sehat terhadap Frambusia pada Anak-anak

Kasus frambusia yang tercatat di Puskesmas Bondo Kodi Kabupaten Sumba Barat Daya, Nusa Tenggara Timur (NTT) terus meningkat dari 174 kasus tahun 2009 menjadi 327 kasus pada tahun 2010 dan 369 kasus pada tahun 2011. Pada tahun 2012, frambusia tertinggi terjadi di Desa Mali Iha di Kecamatan Bondo Kodi dengan 43 kasus. Penelitian ini bertujuan untuk mengetahui faktor-faktor lingkungan, perilaku, dan pengetahuan masyarakat yang berhubungan dengan kejadian penyakit frambusia pada anak-anak. Penelitian observasional ini menggunakan rancangan studi kasus-kontrol, dengan kondisi sarana air bersih (SAB), perilaku hidup bersih dan sehat (PHBS), dan pengetahuan masyarakat tentang frambusia sebagai variabel bebas. Sampel penelitian adalah 30 orang anak yang menderita frambusia (kasus) dan 30 orang anak sehat (kontrol) yang diambil dengan metode purposive sampling. Data dan informasi mengenai SAB, praktik PHBS, dan pengetahuan masyarakat tentang frambusia didapatkan dengan observasi dan wawancara, kemudian dianalisis dengan uji kai kuadrat. Ditemukan, secara statistik kejadian frambusia berhubungan bermakna dengan kondisi SAB (OR = 15,16 dan nilai p = 0,035) dan PHBS (OR = 7 dan nilai p = 0,048), tetapi tidak berhubungan dengan pengetahuan masyarakat tentang frambusia (nilai p = 0,283). Penelitian ini menyimpulkan bahwa kondisi SAB dan PHBS merupakan faktor risiko frambusia.Frambusia cases recorded at Bondo Kodi Primary Health Care in Sumba Barat Daya District, East Nusa Tenggara (NTT) were continously increasing from 174 in 2009 to 327 in 2010 and 369 in 2011. In 2012, the highest frambusia occurred in Mali Iha Village with 43 cases. The present research was to define environmental, behavioural, and knowledge factors associated with the frambusia in children. This observational study employed case-control design with condition of clean water source, practices of personal hygiene and health behavior, and community knowledge about frambusia as independent variables. Samples were 30 children with frambusia (cases) and 30 healthy children (control) who were selected using purposive sampling. Data and information on environmental condition, behavioral practices, and community knowledge were collected by interview and direct observation and were analyzed using chi-square test. It was found that statistically the frambusia cases were associated significantly with the condition of clean water source (OR = 15.16, p value = 0.035) and personal hygiene and healthy behavior (OR = 7, p value = 0.048), but were not associated with community knowledge (p value = 0.283). It concludes that condition of clean water sources and personal hygiene and healthy behavior are risk factors of frambusia in children

The Study of Composting TIME Period by Using Tofu Cesspool Activator\u27s and Fish Cesspool Activator\u27s

      Tofu and fish cesspool has contained an organic substance for microorganism growth in decomposing process of organic substances. The purpose of this research was to know the ability of tofu cesspool activators and fish cesspool activators in composting process.      The research type was pre-experiment research and used posttest only design. The independent variables are tofu cesspool activator and fish cesspool activator. Dependent variables are composting period time, temperature, pH, humidity, and C/N ratio. Data collected were measured result of parameter area, and laboratory test. The data was analyzed descriptively.      Research result indicated that in tofu cesspool activator treatment got pH, 6,9, temperature was 34,7ᴼC, humidity was 64,9% RH and C/N ratio was 13,83% and needed 12 days composting. For group of fish cesspool activator treatment got pH 7, temperature was 35,4ᴼC, humidity was 66,8%RH and C/N ratio was 17,5% and needed 14 days composting. Based on some literatures descripted best temperature was 25ᴼC-60ᴼC, and best Ph about 6.5-7.5, optimum humidity 40-60% RH, period time of composting was 2-3 weeks, and C/N best ratio was 10-20%.      Activator of tofu cesspool\u27s and fish cesspool\u27s can be used in composting process. It was suggested to community to use both activators in composting

Personal Hygiene and Worm Eggs at Pupils Nail in Kupang City, Indonesia

Worm disease does not cause death but can affect digestion, absorption and metabolism of food. Helminthiasis figure in Kupang City in particular were not recorded properly, but when viewed from its risk factor then it was endemic because of sanitary conditions, personal hygiene, behavior, and the provision of clean water is still low. This study aim to identify the presence of worm\u27s eggs in the pupils nail and the correlation with clean and healthy living behavior of pupils. This analytic observational study used cross sectional design. The samples were primary school students as many as 50 people that were taken by random sampling. Data collection was through interviews and laboratory tests on stool samples and analyzed by using chi-square. Results found as many as 13 pupils (26%) were infested by worm eggsof Ascaris lumbricoides at their nail. There were no relation between the presence of worm eggs at pupils nail with gender (p=0.303), age (p=0.747), grade (p=0.643) and playing habits (p=1.000), but there is an association between the presence of worm eggs at elementary pupils nails with the latrine use (p=0.049) and personal hygiene (p=0.039). So that PHC expected to cooperate with the department of education improve the promotion and provision of facilities to support a clean and healthy behaviors for elementary school students