Microcup Arrays for the Efficient Isolation and Cloning of Cells

Arrays of transparent, releasable micron-scale structures termed “microcups” were created for the purpose of patterning and isolating viable cells from small cell samples. Cells were captured by the microcups without the need for barriers or walls on the intervening substrate. Furthermore, in contrast to prior methods for creating cell arrays with releasable elements, no chemical modification of the substrate was required. Individual microcups were released from the array using a pulsed laser at very low energy. Improvements in microcup design enabled cells in suspension to be loaded into the microcups with greater than 90% efficiency. Cells cultured within the microcups displayed 100% viability and were cultured over 4 days yielding colonies that remained sequestered within the microcups to generate pure clonal populations. Standard microscopic imaging was used to identify cells or colonies of interest, and the microcups containing these cells were then released and collected. Individual target cells isolated in this manner remained viable as demonstrated by clonal expansion of 100% of collected cells. Direct comparisons with cell isolation by fluorescence-activated cell sorting and magnetic-bead-based isolation systems demonstrated that the microcup cell isolation procedure yielded higher purity, yield and viability than these standard technologies when separating samples with small numbers of cells. The power of this technique was demonstrated by the isolation of hematopoietic stem cells from a human bone marrow aspirate possessing only 4,000 total cells

Using nonfat milk solids. Part III, Yeast bread and rolls

Cover title

Evaluating Medical Marijuana Dispensary Policies: Spatial Methods for the Study of Environmentally-Based Interventions

This is the peer reviewed version of the following article: Freisthler, B., Kepple, N.J., Simms, R., & Martin, S. (2013). Evaluating Medical Marijuana Dispensary Policies: Spatial Methods for the Study of Environmentally-Based Interventions. American Journal of Community Psychology, 51(1-2), 278-288. doi:10.1007/s10464-012-9542-6, which has been published in final form at http://doi.org/10.1007/s10464-012-9542-6. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.In 1996, California was the first state to pass a Compassionate Use Act allowing for the legal use of marijuana for medical purposes. Here we review several current policy and land use environmental interventions designed to limit problems related to the influx of medical marijuana dispensaries across California cities. Then we discuss the special challenges, solutions, and techniques used for studying the effects of these place-based policies. Finally, we present some of the advanced spatial analytic techniques that can be used to evaluate the effectiveness of environmental interventions, such as those related to reducing problems associated with the proliferation of medical marijuana dispensaries. Further, using data from a premise survey of all the dispensaries in Sacramento, this study will examine what characteristics and practices of these dispensaries are related to crime within varying distances from the dispensaries (e.g., 100, 250, 500, and 1000 feet). We find that some security measures, such as security cameras and having a door man outside, implemented by medical marijuana dispensary owners might be effective at reducing crime within the immediate vicinity of the dispensaries

It’s all the same to me!: Copyright, contracts, and publisher self-archiving policies

This article explores how publisher polices that distinguish between differently-formatted versions of an article do not correlate with what copyright law considers to be separate "works". Under copyright law, only substantial differences in -creative expression- will create a separate work; a manuscript has the same copyright as the identical text formatted for printing. Regardless of publisher policies, If authors retain copyright ownership, they can archive any version of an article that they wish

Rights, ethics, accuracy, and open licenses in online collections: What’s “ours” isn’t really ours

This article explores common practices of cultural organizations sharing collection items online, in which rights are claimed as belonging to the organization that do not, or in which the organization creates confusion for users around who owns or can authorize use of an item

“Kids These Days”… May Know More About Copyright Than You

from "Copyright Conversations: Rights Literacy in a Digital World"; Sara R. Benson, Ed.This chapter explores some of the different moral and ethical considerations people bring to issues of copyright, other intellectual property, authorship, ownership, citation, and attribution - and uses that exploration to suggest strategies for sharing information about copyright law that recognize the various interests of different creator and user communities

"Protecting" our works - from what?

Academic library workers can be even stronger partners with academic creators by developing fluency in the many different ways copyright, intellectual property, and credit are discussed both within and outside the academy. This chapter explores one focus of that rhetoric: protection

Lies, Damned Lies, and Copyright (Mis)Information: Empowering Faculty by Addressing Key Points of Confusion

The University of Minnesota Libraries’ Copyright Program surveyed and interviewed faculty, instructors, researchers, librarians, and library employees to document their knowledge of key areas of copyright law that intersect with common academic practices. All respondents were found to have considerable weaknesses and gaps in knowledge around many key issues. The findings show that all campus populations are in need of further education about the complicated issue of fair use. Some of the findings also suggest avenues for improving copyright education efforts, such as targeting misconceptions about the relation of citation to copyright law, and tying instruction on fundamental principles to faculty authors’ ownership interests in their works