Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway

Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation of tubulin can occur in the nucleus. So far, the potential function of nuclear tubulin has remained elusive. In this work, we show that α- and β-tubulins of various organisms contain multiple conserved nuclear export sequences, which are potential targets of the Exportin 1/CRM1 pathway. We demonstrate exemplarily that these NES motifs are sufficient to mediate export of GFP as model cargo and that this export can be inhibited by leptomycin B, an inhibitor of the Exportin 1/CRM1 pathway. Likewise, leptomycin B causes accumulation of GFP-tagged tubulin in interphase nuclei, in both plant and animal model cells. Our analysis of nuclear tubulin content supports the hypothesis that an important function of nuclear tubulin export is the exclusion of tubulin from interphase nuclei, after being trapped by nuclear envelope reassembly during telophase

Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway

Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation of tubulin can occur in the nucleus. So far, the potential function of nuclear tubulin has remained elusive. In this work, we show that α- and β-tubulins of various organisms contain multiple conserved nuclear export sequences, which are potential targets of the Exportin 1/CRM1 pathway. We demonstrate exemplarily that these NES motifs are sufficient to mediate export of GFP as model cargo and that this export can be inhibited by leptomycin B, an inhibitor of the Exportin 1/CRM1 pathway. Likewise, leptomycin B causes accumulation of GFP-tagged tubulin in interphase nuclei, in both plant and animal model cells. Our analysis of nuclear tubulin content supports the hypothesis that an important function of nuclear tubulin export is the exclusion of tubulin from interphase nuclei, after being trapped by nuclear envelope reassembly during telophase

Osetrovatelske intervence v domaci peci

Article in Czech, abstract in both Czech and English. English title: Nursing interventions in home carePříspěvek prezentuje dílčí výsledky pokračujícího projektu IGA MZ ČR, zabývajícího se tvorbou české verze vybran ých částí klasifikace NIC v domácí péči. Klasifikace NIC je mezinárodní klasifikací ošetřovatelských intervencí a aktivit sestry, která zpřehledňuje a dokumentuje autonomní činnosti sester. Ošetřovatelské intervence a aktivity (NIC) byly zvoleny k posouzení expertní skupinou v závislosti na předchozí ošetřovatelské diagnostice 200 klientů agentury domácí péče v Ostravě. Zařazeny byly intervence a aktivity pro ošetřovatelské diagnózy s četností nad 26 % (9 diagnóz). Těmto diagnózám odpovídalo 263 intervencí spolu s 6 356 příslušnými aktivitami sestry. Tento soubor vybraných intervencí NIC byl posouzen akademickými ošetřovatelskými odborníky a expertní skupinou sester s ohledem na platnou českou legislativu a aktuální stav kompetencí sester. Výsledkem je banka 110 ošetřovatelských intervencí dle NIC a 2 150 příslušných aktivit, které byly zaneseny do elektronické dokumentace pro domácí péči. = This paper presents the partial results of an ongoing project dealing with the creation of a Czech version of selected parts of the NIC classification in home care. NIC classification is an international classification of interventions and activities that nurses perform, which streamlines and documents the autonomous nurses' activities. An expert group chose nursing interventions and activities (NIC) that were based on the previous diagnosis of 200 clients from a home care agency in Ostrava for assessment. They included interventions and activities for nursing diagnoses with a frequency above 26 % (9 diagnoses). These diagnoses corresponded to 263 interventions with 6 356 of relevant nurses' activities. This set of selected NIC interventions was assessed by academic nursing specialists and an expert group of nurses with regard to the valid Czech legislation and the current state of competencies of nurses. As a result, there is a database of 110 nursing interventions according to the NIC and 2150 activities, which were entered into the electronic documentation for home care.Jarošová, D., Sikorová, L., Marečková, J., Pajorová, H

Elektronicka dokumentace pro osetrovatelskou diagnostiku v domaci peci

Article in Czech. Abstract and keywords also in English. English title is: Electronic documentation for nursing diagnostics in home careOšetřovatelská dokumentace je legislativnĕ definovanou povinnou součástí zdravotnické dokumentace. Je důlezitá zejména v organizacích, kde je primárnĕ poskytována oššetřovatelská péče samostatnĕ pracujícími sestrami, jako je domácí péãe. Stĕžejní fází ošetřovatelského procesu je ošetřovatelská diagnostika pacienta/klienta s využitím standardizované terminologie. Ošetřovatelská diagnózy se všemi příslušnými prvky zakomponovanými v elektronické dokumentaci poskytují systematický rámec pro dokumentování a stanovení prioritních potfieb a problémÛ pacientÛ. âlánek prezentuje pfiípravu a ovûfiování elektronické dokumentace, která bude vyuÏívána v domácí péãI pro sbûr ošetfiovatelských dat, pro zhodnocení zdravotního stavu pacienta a jeho potfieb a pro stanovení ošetřovatelských diagnóz s využitím mezinárodní taxonomie NANDA-Int. = Nursing documentation is legislatively defined an obligatory part of health records. It is particularly important in organizations where it is primary providing nursing care by independently working nurses, such as home care. The pivotal phase of the nursing process is nursing diagnostics of patient/client using a standardized terminology. Nursing diagnosis with all the relevant components compiled in electronic record provides a systematic framework for documenting and identifying priority needs and problems of patients. The article presents the preparation and verification of electronic documentation, which will be used in home care for collecting nursing data, for assessment of the patient's health status and his needs and for determination of the nursing diagnoses using the NANDA-Int.Jarošová, D., Sikorová, L., Marečková, J, Pajorová,