5 research outputs found
Extraction and composition characterisation of amino acids from tung meal
<p>The most desirable content of amino acids (AAs) in the extracted products from tung (<i>Aleurites fordii</i>) meal was 93.88%, which was obtained from shelled tung meal at a hydrolysis temperature of 45°C and a isoelectric precipitation pH value of 4.4. Furthermore, the cytotoxic activity of extracted AAs was also evaluated by MTT assay. Antioxidant activity of extracted AAs was also measured by the DPPH assay. As a result, the high yield of extracted AAs exhibited so low cytotoxic and high antioxidant activity that had the potential use as a functional ingredient.</p
Supercooling Self-Assembly of Magnetic Shelled Core/Shell Supraparticles
Molecular self-assembly
has emerged as a powerful technique for
controlling the structure and properties of core/shell structured
supraparticles. However, drug-loading capacities and therapeutic effects
of self-assembled magnetic core/shell nanocarriers with magnetic nanoparticles
in the core are limited by the intervention of the outer organic or
inorganic shell, the aggregation of superparamagnetic nanoparticles,
the narrowed inner cavity, etc. Here, we present a self-assembly approach
based on rebalancing hydrogen bonds between components under a supercooling
process to form a new core/shell nanoscale supraparticle with magnetic
nanoparticles as the shell and a polysaccharide as a core. Compared
with conventional iron oxide nanoparticles, this magnetic shelled
core/shell nanoparticle possesses an optimized inner cavity and a
loss-free outer magnetic property. Furthermore, we find that the drug-loaded
magnetic shelled nanocarriers showed interesting <i>in vitro</i> release behaviors at different pH conditions, including “swelling-broken”,
“dissociating-broken”, and “bursting-broken”
modes. Our experiments demonstrate the novel design of the multifunctional
hybrid nanostructure and provide a considerable potential for the
biomedical applications
Shape Memory Actuation of Janus Nanoparticles with Amphipathic Cross-Linked Network
Preparation
of nanoscale Janus particles that can respond to external
stimulation and, at the same time, be prepared using an easily achievable
method presents a significant challenge. Here, we have demonstrated
the shape memory of Janus nanoparticles (SMJNPs) with a multifunctional
combination of Janus nanostructure and a shape memory effect, composed
of a well-defined amphipathic sucrose-polyÂ(ε-caprolactone) cross-linked
network. A sudden negative pressure method was first used to prepare
the Janus-shaped nanoparticles (temporary shape), which can switch
their shape and wettability. The Janus-shaped nanoparticle is an amphipathic
structure composed of hydrophilic and hydrophobic parts. Moreover,
in response to temperature, the nanoparticle can recover their nanosphere
state via a shape memory process. The novel Janus nanoparticles with
the shape memory property also show a great potential for application
such as drug delivery
Bioadhesive Microporous Architectures by Self-Assembling Polydopamine Microcapsules for Biomedical Applications
Bioadhesive microporous architectures
that mimic the functions
of a natural extracellular matrix (ECM) were prepared by self-assembling
polydopamine (PDA) microcapsules, which not only favor cell adhesion
and growth, but also facilitate growth factor immobilization and release.
PDA-coated polystyrene (PS) microspheres are synthesized by polymerization
of dopamine on sulfonated PS microspheres and then assembled using
positively charged chitosan (CHI) layers as link agents. After the
PS core templates were removed, microporous architectures composed
of PDA microcapsules were obtained. The produced microporous PDA architectures
have a high capability of adsorbing BMP-2 and realize the sustained
release of BMP-2. More importantly, the bioadhesive micro architecture
and its immobilized BMP-2 synergistically enhance the activity and
osteogenetic differentiation of bone marrow mesenchymal stem cells
(BMSCs). Both supercell adhesion and BMP-2 immobilization ability
of these architectures are attributed to the intrinsic adhesive nature
of PDA and the porous architectures via the assembly of PDA microcapsules.
The bioadhesive microporous PDA architectures with both cell affinitive
and GF release features have a great potential to mimic natural ECM
for modifying various medical devices in the fields of tissue engineering
and regenerative medicine
Enhanced Repairing of Critical-Sized Calvarial Bone Defects by Mussel-Inspired Calcium Phosphate Cement
The goal of this
study is to investigate the biological response
of mussel-inspired calcium phosphate cement (CPC) in vivo. Polydopamine
(PDA), which is analogous to that of mussel adhesive proteins, was
added in CPC. PDA-CPC was implanted into the femur, muscle, and critical-sized
calvarial bone defects of rabbits. Histomorphometry of the sequential
fluorescence sections showed that PDA-CPC was capable of forming more
newborn bone than the control-CPC. More new bone, bone marrow cavity,
and blood vessel were observed in PDA-CPC than in the control-CPC
in decalcified and undecalcified histological sections. Necrosis bone
was not observed in PDA-CPC, whereas it appeared in the control-CPC
after 2 weeks. The histological sections in muscle witnessed that
there was more ingrowth of collagen in PDA-CPC than that in the control-CPC.
There were no significantly difference in the number of leukocyte
between PDA-CPC and the control-CPC in blood. It was confirmed that
the addition of PDA enhanced the bone repairing ability and biocompatibility
of PDA-CPC. Push-out testing indicated that PDA increased the bonding
strength between PDA-CPC and host bone in the early stage. These present
results indicated that PDA-CPC might be one potential bone graft with
gratifying biocompatibility and enhanced bone repairing