1 research outputs found
Sorting Short Fragments of Single-Stranded DNA with an Evolving Electric Double Layer
We demonstrate a new procedure for separation of single-stranded
DNA (ssDNA) fragments that are anchored to the surface of a gold electrode
by end hybridization. The new separation procedure takes advantage
of the strong yet evolving nonuniform electric field near the gold
surface in contact with a buffer solution gradually being diluted
with deionized water. Separation of short ssDNA fragments is demonstrated
by monitoring the DNA at the gold surface with <i>in situ</i> fluorescence measurement. The experimental results can be rationalized
with a simple theoretical model of electric double layer that relates
the strength of the surface pulling force to the ionic concentration
of the changing buffer solution