5 research outputs found
Robust estimation of bacterial cell count from optical density
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data
A Parallel Strategy for High-speed Interpolation of CNC Using Data Space Constraint Method
A high-speed interpolation scheme using parallel computing is proposed in this paper. The interpolation method is
divided into two tasks, namely, the rough task executing in PC and the fine task in the I/O card. During the interpolation
procedure, the double buffers are constructed to exchange the interpolation data between the two tasks. Then, the data
space constraint method is adapted to ensure the reliable and continuous data communication between the two buffers.
Therefore, the proposed scheme can be realized in the common distribution of the operation systems without real-time
performance. The high-speed and high-precision motion control can be achieved as well. Finally, an experiment is
conducted on the self-developed CNC platform, the test results are shown to verify the proposed method
Metabolic Dysfunction-associated Fatty Liver Disease is Associated with Greater Impairment of Lung Function than Nonalcoholic Fatty Liver Disease
Background and aims: We compared lung function parameters in nonalcoholic fatty liver disease (NAFLD) and metabolic dysfunction-associated fatty liver disease (MAFLD), and examined the association between lung function parameters and fibrosis severity in MAFLD. Methods: In this cross-sectional study, we randomly recruited 2,543 middle-aged individuals from 25 communities across four cities in China during 2016 and 2020. All participants received a health check-up including measurement of anthropometric parameters, biochemical variables, liver ultrasonography, and spirometry. The severity of liver disease was assessed by the fibrosis (FIB)-4 score. Results: The prevalence of MAFLD was 20.4% (n=519) and that of NAFLD was 18.4% (n=469). After adjusting for age, sex, adiposity measures, smoking status, and significant alcohol intake, subjects with MAFLD had a significantly lower predicted forced vital capacity (FVC, 88.27±17.60% vs. 90.82±16.85%, p<0.05) and lower 1 s forced expiratory volume (FEV1, 79.89±17.34 vs. 83.02±16.66%, p<0.05) than those with NAFLD. MAFLD with an increased FIB-4 score was significantly associated with decreased lung function. For each 1-point increase in FIB-4, FVC was diminished by 0.507 (95% CI: -0.840, -0.173, p=0.003), and FEV1 was diminished by 0.439 (95% CI: -0.739, -0.140, p=0.004). The results remained unchanged when the statistical analyses was performed separately for men and women. Conclusions: MAFLD was significantly associated with a greater impairment of lung function parameters than NAFLD
Metabolic dysfunction-associated fatty liver disease is associated with greater impairment of lung function than nonalcoholic fatty liver disease
Background/aims: we compared lung function parameters in nonalcoholic fatty liver disease (NAFLD) and metabolic dysfunction-associated fatty liver disease (MAFLD), and also examined the association between lung function parameters and fibrosis severity in MAFLD.
Methods: in this cross-sectional study, we randomly recruited 2,543 middle-aged individuals from 25 communities across four cities in China during 2016 and 2020. All participants received a health check-up including measurement of anthropometric parameters, biochemical variables, as well as liver ultrasonography and spirometry. The severity of liver disease was assessed with the fibrosis (FIB)-4 score.
Results: the prevalence of MAFLD and NAFLD was 20.4% (n=519) and 18.4% (n=469), respectively. After adjustment for age, sex, adiposity measures, smoking status and significant alcohol intake, subjects with MAFLD had a significantly lower percent predicted forced vital capacity (FVC: 88.27±17.60% vs. 90.82±16.85%, P<0.05) and lower forced expiratory volume in one second (FEV1: 79.89±17.34 vs. 83.02±16.66%, P<0.05) than those with NAFLD. Furthermore, MAFLD with increased FIB-4 score was significantly associated with decreased lung function parameters, i.e. for each 1-point increase in FIB-4, FVC was diminished by -0.507 (95% CI [-0.840, -0.173], P=0.003) and FEV1 was diminished by -0.439 (95% CI [-0.739, -0.140], P=0.004), after adjustment for sex, age, adiposity measures, smoking status, significant alcohol intake, pre-existing diabetes and other potential confounding factors. The results remained unchanged even when we performed separate statistical analyses for men and women.
Conclusions: MAFLD is significantly associated with a greater impairment in lung function parameters than NAFLD