Stage-specific ethanol exposure causes correlative face and brain abnormalities.

<p>Regression lines were plotted for the control group (solid line) and for the ethanol exposure groups (dashed line). In the exposed groups, a significant negative correlation between philtrum length and septal region volume, along with a significant positive correlation between snout width and olfactory bulb volume, was found. Correlations in the control group were not significant (p = 0.55, p = 0.30, respectively).</p

Study population.

<p>Study population.</p

DSM illustrates unique facial phenotypes resulting from stage-specific ethanol exposure.

<p>(Left) Mean surface shape of the GD7 and GD8.5 exposure groups relative to the control group is shown in the first and second columns respectively, while the GD7 group is directly compared to the GD8.5 ethanol-exposed group in the third column. (A–C) Color-map comparisons reflecting the displacement of mean surface shape for the indicated groups, where red indicates regions most distant and internal, while blue indicates regions most distant and external. Other colors shown in the scales identify intermediate positions. (D–L) Color-map comparisons reflecting the displacement of the indicated mean surface shapes parallel to the three orthogonal axes. Red and blue color intensities reflect displacement in the direction indicated by the corresponding color-coded arrow. Changes are shown at a scale of 1.2 standard deviations. (Right) Snout width (SW) was measured between the most lateral 3^rd row of vibrissae; Median upper lip length (ULL) was measured from the lower edge of the nostrils to the bottom of the upper lip; Facial depth (FD) was measured from the middle of the ear to the top of the philtrum. Values represent the means + the S.E.M. ^*p<0.05 compared to control group.^∧p<0.05 compared to counterpart ethanol exposure group.</p

Unique facial dysmorphology induced by stage-specific ethanol exposure in the mouse corresponds to distinct clinical phenotypes.

<p>Along with a control animal (A), representative examples of fetuses severely affected by ethanol exposure on GD7 (B) and GD8.5 (C) are shown. The elongated upper lip with deficient philtrum of the GD7 exposed mouse mimics that seen in children with full-blown FAS <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043067#pone.0043067-Sulik3" target="_blank">[50]</a>. The “fish-shaped” upper lip and bulbous nasal tip of the mouse exposed at GD8.5 resembles that of children with DiGeorge syndrome <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043067#pone.0043067-Kretschmer1" target="_blank">[37]</a>. For the animals shown, the face and brain can be visualized concurrently in 3D in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043067#pone.0043067.s008" target="_blank">Movie S1</a>.</p

Scanning electron microscopy illustrates unique brain malformations in GD12 embryos exposed to ethanol at GD7 or GD8.5.

<p>(A–F) Images of specimens hemisected in the coronal (frontal) plane illustrate posterior and anterior aspects of the embryonic brain in control and ethanol-affected groups. (G–I) Additional GD12 embryos were cut to provide a sagittal view of the brain. Notable abnormalities include differences in width of the third ventricle (dashed calipers), and the area from which the septal region will develop (dashed outline in the anterior view and solid calipers in sagittal view). Ganglionic eminences (*).</p

MRM enables concurrent 3D analyses of the brain and face of GD17 mouse fetuses.

<p>Forebrain and pituitary regions were manually segmented from transverse 39 µm MRM sections (A). 3D brain reconstructions were generated by overlaying manually segmented regions with whole-brain masks (B). From the same MRM scans, 3D head reconstructions were created featuring detailed facial surfaces (C). The brain and face can be visualized concurrently <i>in situ</i> by reducing head surface opacity (D). The size of a GD17 mouse fetus can be appreciated when shown in scale with a U.S. quarter dollar coin (E).</p

Volumetric brain measurements demonstrate unique brain abnormalities resulting from stage-specific ethanol exposure.

<p>(Right) Total brain volumes were derived from automated skull stripping. Values represent the mean + S.E.M. Letters above each bar indicate group classes; the same letter above a subset of bars denotes lack of statistical difference, whereas different letters represent statistically different classes (p<0.05). (Right) For determination of disproportionate differences, the volume of each manually segmented forebrain region was calculated as a percentage of total brain volume for each animal. Remaining volume includes mid- and hindbrain regions. To illustrate relative changes on the same scale, percent volumes are normalized to mean control values. Values represent the mean ± the S.E.M. ^*p<0.05 compared to control group. ^∧p<0.05 compared to counterpart ethanol exposure group.</p