Additional file 9 of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Table S6. Donor demographics. (XLSX 41 kb

Additional file 5: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Table S4 DESeq2 analysis results for genes with uncorrected P < 0.05, between AD and controls. (XLSX 229 kb

Additional file 3: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Figure S1. Circular-to-linear ratios. Ratio of average back-spliced reads to average linearly spliced reads for all detected circRNAs. (PDF 1075 kb

Additional file 7: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Figure S3. Computational workflow outline and filtering criterion. PC, posterior cingulate; RNAseq, RNA sequencing; circRNA, circular RNA; miRNA, microRNA; mRNA, messenger RNA. (PDF 928 kb

Additional file 2: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Table S2. Circular-to-linear ratios for all detected circRNAs (XLSX 342 kb

Additional file 1: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Table S1. Master summary of all detected circRNAs (XLSX 559 kb

Additional file 10: of Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects

Table S7. Tool parameters used for circRNA detection in this study. (XLSX 45 kb

mRNA expression analysis of EWAS-related genes in AD CA1 pyramidal neurons AD CA1 astrocytes and AD CA1 microglia.

<p>Only two of the seven identified transcripts in the EWAS study were significantly differentially expressed, <i>BIN1</i> in AD neurons and <i>SERPINF2</i> in AD microglia. * indicates p < .05.</p

<i>ANK1</i> mRNA expression levels in hippocampal homogenates, AD CA1 pyramidal neurons AD CA1 astrocytes and AD CA1 microglia.

<p>Bar graph depicts log2 fold change, comparing AD vs. age matched normal controls. No significant difference was detected in homogenates, AD neurons, or AD astrocytes. In stark contrast, a significant four-fold increase (<i>p<0</i>.<i>001</i>) was observed in AD microglia.</p

Significant, Log2 fold change(s) in genes containing ankyrin repeat domains in AD hippocampal homogenates, AD CA1 pyramidal neurons, AD CA1 astrocytes and AD CA1 microglia.

<p>A) Significantly <i>(p <</i> .<i>05)</i> altered Ankyrin repeat containing genes in hippocampal homogenates in AD compared to ND. B) Significantly altered (p < .05) Ankyrin repeat containing genes in LCM neurons and glial cells (C). D) average log2 mRNA fold difference in ankyrin repeat genes in PD microglia from CA1 of the hippocampus compared to the same matched control subjects used in AD comparisons. LCM neurons, astrocytes and microglia were derived from the same human subjects. Detailed expression changes can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0177814#pone.0177814.s002" target="_blank">S1 Fig</a>.</p