ЭНЕРГОДЕФИЦИТ ТРОМБОЦИТОВ У БОЛЬНЫХ С ОСТРЫМ КОРОНАРНЫМ СИНДРОМОМ В СОЧЕТАНИИ С ТРЕВОЖНО-ДЕПРЕССИВНЫМИ РАССТРОЙСТВАМИ

Purpose. We study of enzyme activity of blood platelets in patients with acute coronary syndrome (ACS) in a combination with anxiety and depressive disorders (ADD ).Materials and methods. We examined 315 patients of both sexes in the first 24 hours of the ACS. There were 154 ACS patients without ADD and 161 patients with ADD . We researched the activity of the NAD and NADP-dependent dehydrogenases in platelets of the patients using bioluminescence assay methods in the first 24 hours after the patients’ admission and in the disease progress on the 10th day.Results. A distinctive feature of the platelet metabolism in patients with ACS without ADD is a decreased level of aerobic respiration activity accompanied with the violation of interrelation between the Krebs cycle and reactions of amino acid metabolism while anaerobic respiration intensity is at a normal level. Patients who had ACS with ADD at all the stages of the examination had decreased activity of both anaerobic and aerobic respiration beside the disrupted connection between the products of amino acid metabolism and the Krebs cycle reactions.Conclusion. ACS patients with ADD had a more pronounced change in the platelet energy metabolism, which may be one of the pathophysiological causes of the violation of the functional platelet activity in the hemostasis.Цель. Изучение активности ферментов тромбоцитов у больных с острым коронарным синдромом (ОКС) в сочетании с тревожно-депрессивными расстройствами (ТД Р).Материалы и методы. Обследовано 315 пациентов обоего пола в первые 24 часа от развития острого коронарного синдрома. Были сформированы две группы: первая – больные с ОКС с ТД Р (n=161) и вторая – больные с ОКС без ТД Р (n=154). В первые 24 часа после госпитализации пациентов и в динамике заболевания на 10-е сутки с помощью методов биолюминесцентного анализа проведено исследование активности НАД- и НАДФ-зависимых дегидрогеназ в тромбоцитах больных.Результаты. У больных с ОКС без ТД Р выявлено снижение уровня активности аэробного дыхания на фоне нарушения взаимосвязи цикла Кребса с реакциями аминокислотного обмена, но при сохранении нормального уровня интенсивности анаэробного дыхания. У больных с ОКС с ТД Р на всех этапах обследования выявлено снижение активности анаэробного и аэробного дыхания на фоне сниженного переноса продуктов аминокислотного обмена на реакции цикла Кребса.Выводы. Таким образом, у больных с ОКС с ТД Р выявлено более выраженное изменение энергометаболизма, что может являться одной из патофизиологических причин нарушения функциональной активности тромбоцитов в процессах гемостаза

HEMOSTASIS AND FUNCTIONAL PROPERTIES OF NEUTROPHILS IN PATIENTS WITH VARIOUS SENSITIVITY TO ACETYLSALICYLIC ACID IN ACUTE CORONARY SYNDROME

Aim. To study the specifics of relation between hemostasis parameters and functional activity of neutrophil granulocytes in sensitive and resistant to acetylsalicylic acid (ASA) patients with acute coronary syndrome.Material and methods. Totally 53 patients included during the first 24 hours of ACS. Controls consisted of 50 relatively healthy individuals. All patients before the treatment and revascularization were assessed for the resistance to ASA, and selected into groups of sensitive (AS) and resistant (AR). Sensitivity test was done in vitro. In all participants the coagulation and platelet clotting systems were studied. With the luminescent method the neutrophil activity was assessed. Results. In AR with ACS there was decrease of platelets in blood, decreased level of ADP- (ADP 5 mcM) and adrenalin-induced aggregation, increases the level of ADP-induced platelets aggregation (PA) with ADP 0,1 mcM, and von Willebrand factor level. In the ASA sensitive patients there was a decrease of adrenalin induced aggregation.There were no specifics of coagulation hemostasis according to ASA sensitivity. Chemiluminiscence (CL) of neutrophils in ACS has a maximized out time. In AR there was decrease of activation. In AS functional activity of neutrophils more significantly correlates with the parameters of clotting, but in AR — with fibrinolytic and anticoagulation parameters.Conclusion. In ACS patients depending on ASA sensitivity, there are character specifics of platelet clotting: increase of ADP-induced aggregation in AR, decrease of platelet amount and the level of von Willebrand factor. In ACS patients there is a decreased velocity of "respiratory boost" in neutrophils. In AR there is more prominent dysbalance of coagulation hemostasis and "respiratory boost" of neutrophils, with the decrease of neutrophil reactivity related to clotting activity and appearance of relations with the parameters of fibrinolytic and anticoagulation activity

DYNAMICS OF HEMOSTASIS AND NEUTROPHIL FUNCTIONAL ACTIVITY CHANGES IN PATIENTS WITH VARIOUS SENSITIVITY TO ACETYLSALICYLIC ACID IN ACUTE MYOCARDIAL INFARCTION

Aim. To study in dynamics the specifics of hemostasis and functioning of neutrophilic granulocytes in patients with acute myocardial infarction (MI) sensitive and resistant to acetylsalicylic acid (ASC).Material and methods. Totally, 53 patients included during first 24 hours from MI onset. Controls were 50 relatively healthy volunteers. All patients before treatment and revascularization were assessed for resistance to ASC and selected to groups as sensitive (SASC) and resistant (RASC). In complex therapy there was double antiplatelet therapy (ASC+clopidogrel). The parameters of vessel-platelet and plasma hemostasis were assessed. Via the chemiluminescent analysis we studied functional activity of neutrophils.Results. In RASC patients with MI on 1st and 10th day we found increased ADP aggregation of thrombocytes (ADP 0,1 mcM) and increase of von Willebrand factor. On 10th day in SASC patients there was decrease of ADP aggregation (ADP 5 mcM), von Willebrand factor increases. Not related to ASC sensitivity, in MI patients there is increase of SFMC, thrombin time is increased and D-dimer is low. Kinetics of chemiluminescence of neutrophils in MI patients differs from controls only by increased time of reached maximum. In ASC resistant patients on 1st day of evaluation there is decrease of neutrophil activation index, which gets to normal by 10th day of treatment.Conclusion. In RASC MI patients there is increased ADP aggregation of platelets, decrease of platelets number and increase of von Willebrand factor levels. Thrombosis risk in RASC patients on 10th day of treatment is caused by remaining disordered vessel-platelet hemostasis and increased spontaneous platelets aggregation. Plasmatic hemostasis on 1st and 10th days of MI shows activated onset of clotting with increase of clotting duration and significant decrease of D-dimer. In MI there is slowed velocity of development of “respiratory boost” in neutrophilic granulocytes that is probably related to compensatory processes in organism and double antiplatelet therapy which is antiinflammatory as well

Correlating solution binding and ESI-MS stabilities by incorporating solvation effects in a confined cucurbit[8]uril system.

The high-throughput characterization of solution binding equilibria is essential in biomedical research such as drug design as well as in material applications of synthetic systems in which reversible binding interactions play critical roles. Although isothermal titration calorimetry (ITC) has been widely employed for describing such binding events, factors such as speed, concentration, and sample complexity would principally favor a mass spectrometry approach. Here, we show a link between ITC and electrospray ionization mass spectrometry (ESI-MS) by incorporating solvation free energies in the study of the ternary complexes of the macrocyclic host cucurbit[8]uril (CB[8]). The binding affinities of 32 aromatic reference complexes were studied by ITC and ESI-MS and combined with solvation data of the guests from an implicit solvation model (SM8) to obtain a correlation between aqueous and gas-phase measurements. The data illustrates the critical importance of solvation on the binding strength in CB[8]'s ternary complexes. Finally, this treatment enabled us to predict association constants that were in excellent agreement with measured values, including several highly insoluble guest compounds.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe