EVALUATION OF IN VITRO ANTICANCER ACTIVITY OF SYMPLOCOS RACEMOSA BARK AGAINST HEPATOCELLULAR CARCINOMA

Objective: To investigate in vitro anticancer activity of different extracts of bark of Symplocos racemosa against hepatocellular carcinoma.Methods: Different successive extracts of Symplocos racemosa bark were prepared using hexane, chloroform, ethyl acetate, n-butanol and water and were tested in vitro for cytotoxicity using (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay in rat normal liver cells (BRL-3A) and human hepatocellular carcinoma (Hep3B) cells.Results: Ethyl acetate and chloroform extract of Symplocos racemosa exhibited cytotoxicity against human hepatocellular carcinoma (Hep3B) cells in vitro with IC50 value (µg/ml) of 63.45 and 75.55 respectively and not affected the normal liver (BRL-3A) cells.Conclusion: Symplocos racemosa bark extracts showed potential cytotoxic effects on human hepatocellular carcinoma cells. The anticancer activity exhibited by ethyl acetate and chloroform extract might be due to presence of phenolics and flavonoid constituents present in the bark. Ethyl acetate extract can further be explored for possible cytotoxic activity using in vivo models of liver cancer.Â

Effect of Covid-19 Pandemic on Shrimp Farming at Navsari, Gujarat, India

The lockdown on account of the Coronavirus disease 2019 (COVID-19) adversely impacted the food production sector including aquaculture globally. Unfortunately, it coincided with the major shrimp farming season in India which contributes 60% of the national annual shrimp production hence the impact was substantial. Shrimp farmers follow the different management practices in shrimp farming operations like seed, feed, probiotics, medicine, disruptions in the supply chain, transportation, harvesting and marketing. Therefore, the objective of present study was to find out the impact of COVID-19 pandemic on shrimp farming sector in Navsari (Gujarat). These findings are depicted that management practices of the shrimp farming in study area was disturbed and huge economic losses was faced by the shrimp farmers during pandemic situations. It is suggested that technical and financial assistance for safe, sufficient and hygienic supply of seed, feed, probiotics, chemicals, medicines and probiotics by the government agencies should be ensured which help to shrimp enhance the aquaculture production and survive of shrimp farmers. The result of present study is also helpful for the government authorities to develop policies and execution of the plans during such pandemic condition for shrimp farmers that can prevent the production loss and sustain the shrimp farming industry

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes