Additional file 2: Figure S1. of Fermented barley and soybean (BS) mixture enhances intestinal barrier function in dextran sulfate sodium (DSS)-induced colitis mouse model

BS promotes recovery from LPS-induced colitis and inflammation in mice. (A) DAI of LPS (LPS, 5 mg/kg of mouse by Intra-peritoneal injection) treated mice were scored. (B) Representative H&E stained histology from Control and BS-treated groups and statistical results. Mean value was significantly different from that of the LPS treated control group (*P < 0.05). BS, Fermented barley and soybean, DAI, disease activity index. LPS, Lipopolysaccharide. Figure S2. Basal intestinal barrier function in BS treatment. (A) Immunofluorescence of ZO-1 in Caco-2 cell monolayers incubated with BS for 48 h and images were collected by confocal microscopy. (B) RNA levels of ZO-1, Claudin1, and Occludinin BS treated Caco-2 cells measured by semi-quantitative RT-PCR. (C) BS treated mouse colon tissues were used to determine ZO-1, Claudin1 and Occludindistribution by immune fluorescence staining and images were collected by confocal microscopy. BS, Fermented barley and soybean. Figure S3. Effect of BS on the suppression of NF-κBactivity. (A) Western blot analysis of p65 nuclear trans-localization levels in the protein fractions of RAW 264.7 lysates. (B) The effects of BS on a NF-κBreporter assay in HEK 293 cells that were activated by the addition of LPS. The luciferase activity was measured as relative light intensity, using a plate reader in the luminescence mode. BS, Fermented barley and soybean, LPS, Lipopolysaccharide. Figure S4. Immunohistochemicalstaining of IL-6 in sections from BS treated mouse colon. BS, Fermented barley and soybean. (PDF 563 kb

Additional file 1: Table S1. of Fermented barley and soybean (BS) mixture enhances intestinal barrier function in dextran sulfate sodium (DSS)-induced colitis mouse model

Primer sets used for semi-quantitative PCR of cytokines. Table S2. Primer sets used for quantitative PCR of 16S rRNAof specific species or genus. (PDF 105 kb