Trabecular Meshwork Engineering and Live Tracking in Perfused Porcine Anterior Segments

Purpose: To establish a trabecular meshwork ™ engineering model using porcine anterior segments of consistently high quality in a physiological, fixed perfusion system.\ud \ud Discussion: Compared to previously used human donor eyes, this inexpensive porcine anterior segment perfusion model is of sufficient, repeatable high quality to develop strategies to modify genetically, ablate and repopulate the TM. Despite significant anatomic differences, effects of transduction and ablation in the porcine model presented here replicate key aspects of previously explored human, feline and rodent models

Molecular Events in Lamin B1 Homopolymerization: A Biophysical Characterization

Lamin B1 is one of the major constituents of the nuclear lamina, a filamentous network underlying the nucleoplasmic side of the inner nuclear membrane. Homopolymerization of lamin B1, coupled to the homotypic and heterotypic association of other lamin types, is central to building the higher order network pattern inside the nucleus. This in turn maintains the mechanical and functional integrity of the lamina. We have characterized the molecular basis of the self-association of lamin B1 using spectroscopic and calorimetric methods. We report that concentration dependent lamin B1 oligomerization involves significant alterations in secondary and tertiary structures of the protein resulting in fairly observable compaction in size. Comparison of the energetics of the homotypic association of lamin B1 with that of lamin A reported earlier led to the finding that lamin A oligomers had higher thermodynamic stability. This leads us to conjecture that lamin B1 has less stress bearing ability compared to lamin A

Viscoelastic Behavior of Human Lamin A Proteins in the Context of Dilated Cardiomyopathy

Lamins are intermediate filament proteins of type V constituting a nuclear lamina or filamentous meshwork which lines the nucleoplasmic side of the inner nuclear membrane. This protein mesh provides a supporting scaffold for the nuclear envelope and tethers interphase chromosome to the nuclear periphery. Mutations of mainly A-type lamins are found to be causative for at least 11 human diseases collectively termed as laminopathies majority of which are characterised by aberrant nuclei with altered structural rigidity, deformability and poor mechanotransduction behaviour. But the investigation of viscoelastic behavior of lamin A continues to elude the field. In order to address this problem, we hereby present the very first report on viscoelastic properties of wild type human lamin A and some of its mutants linked with Dilated cardiomyopathy (DCM) using quantitative rheological measurements. We observed a dramatic strain-softening effect on lamin A network as an outcome of the strain amplitude sweep measurements which could arise from the large compliance of the quasi-cross-links in the network or that of the lamin A rods. In addition, the drastic stiffening of the differential elastic moduli on superposition of rotational and oscillatory shear stress reflect the increase in the stiffness of the laterally associated lamin A rods. These findings present a preliminary insight into distinct biomechanical properties of wild type lamin A protein and its mutants which in turn revealed interesting differences

Structural Alterations of Lamin A Protein in Dilated Cardiomyopathy

Lamin A protein, encoded by the <i>LMNA</i> gene, belongs to the type V intermediate filament protein family and is a major nuclear protein component of higher metazoan organisms, including humans. Lamin A along with B-type lamins impart structural rigidity to the nucleus by forming a lamina that is closely apposed to the inner nuclear membrane and is also present as a filamentous network in the interior of the nucleus. A vast number of mutations that lead to a diverse array of at least 11 diseases in humans, collectively termed laminopathies, are being gradually uncovered in the <i>LMNA</i> gene. Dilated cardiomyopathy (DCM) is one such laminopathy in which ventricular dilation leads to an increase in systolic and diastolic volumes, resulting in cardiac arrhythmia and ultimately myocardial infarction. The point mutations in lamin A protein span the entire length of the protein, with a slight preponderance in the central α-helical coiled-coil forming domain. In this work, we have focused on three such important mutations that had been previously observed in DCM-afflicted patients producing severe symptoms. This is the first report to show that these mutations entail significant alterations in the secondary and tertiary structure of the protein, hence perturbing the intrinsic self-association behavior of lamin A protein. Comparison of the enthalpy changes accompanying the deoligomerization process for the wild type and the mutants suggests a difference in the energetics of their self-association. This is further corroborated by the formation of the aggregates of different size and distribution formed inside the nuclei of transfected cells

Viscosity of wild type and mutant lamin A networks under shear.

<p>A) Steady shear viscosity (<i>η</i>) as a function of shear-rate denoted by open circles and complex viscosity ((ω)) as a function of angular frequency (<i>ω</i>) denoted by solid circles are shown. The inset shows the flow curve of wt LA (0.7 mg/ml) indicating the variation of shear stress (<i>σ</i>) with shear-rate. B) Steady shear viscosity (<i>η</i>) as a function of shear-rate and C) complex viscosity ((ω)) as a function of angular frequency (<i>ω</i>) of 0.7 mg/ml of wt LA and 0.6 mg/ml of E161K and R190W. D) Flow curve indicating the variation of shear stress (<i>σ</i>) with shear-rate of 0.7 mg/ml of wt LA and 0.6 mg/ml of E161K and R190W.</p

Seasonal Transition in PM₁₀ Exposure and Associated All-Cause Mortality Risks in India

Lack of a consistent PM₁₀ (particulate matter smaller than 10 μm) database at high spatial resolution hinders in assessing the environmental impact of PM₁₀ in India. Here we propose an alternate approach to estimate the PM₁₀ database. Aerosol extinction coefficients at the surface are calculated from midvisible aerosol optical depth from MERRA-2 reanalysis data using characteristics vertical profiles from CALIOP and then are converted to PM₁₀ mass using aerosol property information and microphysical data. The retrieved PM₁₀ are bias-corrected and evaluated (<i>R</i>² = 0.85) against coincident ground-based data maintained under the Central Pollution Control Board network. PM₁₀ exposure exceeds Indian annual air quality standard in 72.3% districts. Transition in PM₁₀ exposure from the monsoon (Jun–Sep) to postmonsoon season (Oct–Nov) translates to 1–2% higher all-cause mortality risk over the polluted Indo-Gangetic Basin (IGB). Mortality risk increases in the central to eastern IGB and central India and reduces in Delhi national capital region in the winter (Dec–Feb) relative to the postmonsoon season. Mortality risk decreases by 0.5–1.8% in most parts of India in the premonsoon season (Mar–May). Our results quantify the vulnerability in terms of seasonal transition in all-cause mortality risks due to PM₁₀ exposure at district level for the first time in India

Expression, folding and ultrastructure of lamin A.

<p>A) 10% SDS PAGE analysis of pure fractions of wt LA, E161K and R190W from Mono S column; immunoblot of the same fractions using mouse monoclonal anti lamin A+C antibody (JoL2). Numbers corresponding to the bands of the marker in lane M are in kilo Daltons. B) CD spectra of 0.7 mg/ml wt LA in 4 M urea, 2 M urea and assembly buffer respectively at 25°C. C) SEM images of WT, E161K and R190W at concentrations of 0.6 mg/ml. Magnification for WT and mutants are 2000x and 3000x respectively. Scale bars for wt LA and mutants are 20 µm and 10 µm respectively. Arrow and Asterisk marks indicate the cross-linked sites and bundled filaments in the network respectively. D) Mesh size of lamina from EGFP tagged wt LA and mutants transfected in HeLa cells were calculated from confocal images and represented as box plot (n =  200–300, in 10 nuclei).</p

Strain induced changes in the network of wt LA and mutant protein and DLS measurements.

<p>A) Dependence of elastic modulus <i>G′</i> and viscous modulus <i>G′′</i> of wt LA at 0.85 mg/ml concentration on varying the strain amplitude in the range of 0.01 to 1000%, keeping the angular frequency fixed at 5 rad/s. Green and blue arrow indicates critical strain and yield strain respectively. B) Concentration dependence of the critical strain corresponding to the onset of non-linearity and the yield strain (inset) above which the network starts to flow is shown. Concentrations of 0.6, 0.85 and 2 mg/ml were used for this experiment. C) Dependence of elastic modulus <i>G′</i> and viscous modulus <i>G′′</i> of wt LA and E161K at 0.6 mg/ml concentration on varying the strain amplitude in the range of 0.01 to 1000%, keeping the angular frequency fixed at 5 rad/s. Black and blue arrows indicate critical strain, grey and light blue arrows indicate yield strain of wt LA and E161K respectively. Inset shows a model representing the fate of wt LA and mutant LA network upon shear deformation. D) Number percentage statistics of 0.3 and 3 mg/ml of wt LA protein.</p

Lamin A in lamina acts as a “Check valve” in response to stress.

<p>Inside lamina A and B-type lamins respond differently with increasing cellular stress at different stages of differentiation.</p

Elastic Behaviour of wt LA and mutant proteins.

<p>A) Increase in storage modulus <i>G′</i> and B) loss modulus <i>G′′</i> of wt LA upon assembly in lamin A assembly buffer with increasing concentrations. <i>G′</i> and <i>G′′</i> are the in-phase and out of phase components respectively, of an oscillatory shear of strain amplitude 1% at an angular frequency of 5 rad/s for 1000 – 3000 s. Protein concentrations used were in the range of 0.28–3.2 mg/ml of wt LA. C) Same measurement as in (A) and (B) with wt LA concentration fixed at 2.2 mg/ml and DOPC concentrations in the range 0 – 10 mg/ml. The decrease in <i>G′</i> with different DOPC concentration at the air/water interface is shown in the inset. The <i>G′</i> values obtained from repeated measurements lie within the experimental error bar. D) Concentration dependent increase in <i>G′</i> of wt LA. Comparison of E) Storage modulus <i>G′</i> and F) Loss modulus <i>G′′</i> of wild type and mutants upon assembly in assembly buffer. 0.6 mg/ml concentration of wt LA, E161K and R190W were used for these measurements. The parameters for (E, F) are identical to (A, B).</p