Picosecond pulse radiolysis: Dynamics of solvated electrons in ionic liquid and geminate ion recombination in liquid alkanes

金沢大学理工研究域自然システム学系A picosecond pulse radiolysis facility based on a laser-driven photocathode electron accelerator has been constructed. First observation of picosecond dynamics in ionic liquid of DEMMA-TFSI in radiation chemistry was reported. It is found that the electrons produced by ionization are solvated to full solvation in ionic liquid with a rate constant of 3.9×1010 s-1, and dry electrons before full solvation react rapidly with biphenyl and pyrene with a rate constant of 3.8-7.9×1011 dm3 mol-1 s-1. The geminate ion recombination in n-dodecane and n-hexane was also observed by monitoring transient optical absorption at 523 nm. © 2008 Elsevier Ltd. All rights reserved

ECRプラズマ ニ ヨル a-Si : Hマク ノ コウソクセイマクヨウ ハンノウソウチ ト セイセイマク ノ セキガイキュウシュウ スペクトラム ノ カンイテイリョウ ブンセキホウ

A novel microwave plasma reactor for high rate deposition of a-Si : H films is described with a special attention paid on decreasing plasma maintenance power. The reactor is a coaxial-line type composed of stainless inner conductor and mesh outer conductor covering a quartz tube. Secondary electron emission and ECR effects decrease drastically the plasma maintenance power. Furthermore simple quantitative analysis of infrared absorption spectra of the a-Si : H films is presented also. This is basically the deconvolution of the spectra to two Gaussian functions, using least sqare method, but it contains analytic solutions, in part, to decrease CPU time

マイクロハ グローホウデン CVD ニ ヨル a-Si:Hハクマク ノ セイセイ

Microwave plasma of SiH_4/H_2 gas has been produced by glow discharge in direction of microwave propagation along a ridge waveguide and investigated by observing distribution of optical emission from the plasma. 0ptimum position of gas inlet has been obtained. Deposited film posseses more or less microcrystalline phase, but its photoelectric property differs whether it is prepared inside or outside the plasma. Photoelectric property of the film obtained inside the plasma is poor but it is improved by hydrogenation or intermittent discharge. The film obtained outside the plasma has fairly good photoelectric property and growth rate of 20Å/s. It is postulated that the former film is affected by high substrate temperature and the latter is grown partly by chemical transport

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma.

Previously, we developed a technique to introduce a superfolder green fluorescent protein (sGFP) fusion protein directly into plant cells using atmospheric-pressure plasma. In this study, we attempted genome editing using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system using this protein introduction technique. As an experimental system to evaluate genome editing, we utilized transgenic reporter plants carrying the reporter genes L-(I-SceI)-UC and sGFP-waxy-HPT. The L-(I-SceI)-UC system allowed the detection of successful genome editing by measuring the chemiluminescent signal observed upon re-functionalization of the luciferase (LUC) gene following genome editing. Similarly, the sGFP-waxy-HPT system conferred hygromycin resistance caused by hygromycin phosphotransferase (HPT) during genome editing. CRISPR/Cas9 ribonucleoproteins targeting these reporter genes were directly introduced into rice calli or tobacco leaf pieces after treatment with N2 and/or CO2 plasma. Cultivation of the treated rice calli on a suitable medium plate produced the luminescence signal, which was not observed in the negative control. Four types of genome-edited sequences were obtained upon sequencing the reporter genes of genome-edited candidate calli. sGFP-waxy-HPT-carrying tobacco cells exhibited hygromycin resistance during genome editing. After repeated cultivation of the treated tobacco leaf pieces on a regeneration medium plate, the calli were observed with leaf pieces. A green callus that was hygromycin-resistant was harvested, and a genome-edited sequence in the tobacco reporter gene was confirmed. As direct introduction of the Cas9/sgRNA (single guide RNA) complex using plasma enables genome editing in plants without any DNA introduction, this method is expected to be optimized for many plant species and may be widely applied for plant breeding in the future