5 research outputs found
Hidrogeles bioactivos para la regeneraci贸n 贸sea en osteoporosis
El objetivo de este trabajo es la elaboraci贸n, caracterizaci贸n y evaluaci贸n de sistemas nanoestructurados inyectables, a base de hidrogeles preparados con Pluronic庐-F127 y Tetronic庐 1307, conteniendo BMP-2 y 脽-estradiol formulados en micropart铆culas de pol铆meros biodegradables, para su aplicaci贸n en regeneraci贸n de defectos cr铆ticos 贸seos en poblaci贸n osteopor贸tica. La composici贸n de los dos sistemas desarrollados es, en primer lugar, un hidrogel de Pluronic庐-F127, Tetronic庐 1307 y 驴-ciclodextrina (P/T/CD) y, como alternativa, se elabor贸 otro sistema de Tetronic庐 1307 y alginato (T-A). Los sistemas se caracterizaron en cuanto a propiedades reol贸gicas, resistencia a la desintegraci贸n, control de la liberaci贸n de las sustancias activas y eficacia regenerativa in vivo. El P/T/CD mostr贸 su biocompatibilidad en cultivos de c茅lulas mesenquimales de m茅dula 贸sea de rata y su capacidad para facilitar su diferenciaci贸n a osteoblastos. Por otro lado, los coeficientes de difusi贸n de los componentes del sistema (determinados por resonancia magn茅tica nuclear) confirmaron la formaci贸n de un complejo de inclusi贸n pol铆meros-CD. Ambos sistemas gelifican en un intervalo de temperatura comprendido entre 20 掳C y 37 掳C, lo que asegura su inyectabilidad y adaptaci贸n a la forma del defecto. De acuerdo con los estudios de estabilidad estructural in vitro, los sistemas mantienen su integridad, al menos, durante cuatro semanas. Las modificaciones realizadas en el sistema T-A dieron lugar a un mejor control de la liberaci贸n de las sustancias activas y a una mayor resistencia a la erosi贸n. Sin embargo, estas diferencias no se reflejan en mayor eficacia regenerativa. Con ambos sistemas el porcentaje de regeneraci贸n obtenido fue similar, 50-60% del defecto se rellen贸 con tejido 贸seo nuevo. En general, la combinaci贸n de 脽-estradiol y BMP-2 result贸 ser el tratamiento m谩s efectivo
Problem-Based Learning. Application to a Laboratory Practice in the Degree of Industrial Chemical Engineering
The transformation of university teaching towards a competency acquisition approach requires an update of teaching methodologies. In the case of laboratory practices, the application of the Problem-Based Teaching methodology encourages students to apply their knowledge to solve problems based on real situations, as well as efficient communication in a work environment. This paper presents the results obtained by applying this alternative teaching methodology to a laboratory practice in the last year of the Degree in Industrial Chemical Engineering at the University of La Laguna. The results confirm a greater theoretical understanding of the concepts and the ability to apply them in practice, with a notable increase in motivation and interest in their learning process
Enhancement of Peak Flux Capacity in Membrane Bioreactors for Wastewater Reuse by Controlling the Backwashing Strategy
PLGA-BMP-2 and PLA-17尾-Estradiol Microspheres Reinforcing a Composite Hydrogel for Bone Regeneration in Osteoporosis
Mobility of Water and Polymer Species and Rheological Properties of Supramolecular Polypseudorotaxane Gels Suitable for Bone Regeneration
The
aim of this work was to prepare polypseudorotaxane-based supramolecular
gels combining 伪CD with two temperature-responsive copolymers
of different architecture (i.e., linear poloxamer P and X-shaped poloxamine
T), at the lowest concentration as possible to form syringeable depots,
and to shed light on the self-diffusion and spatial closeness of all
components (including water) which in turn may determine the cooperative
self-assembly phenomena and the performance of the gels at the macroscopic
level. The exchange rate between bound water and bulk water was measured
with a novel NMR experiment Water Diffusion Exchange-Diffusion Optimized
Spectroscopy (WDE-DOSY). Polypseudorotaxane formation caused opposite
effects on the mobility of 伪CD species (decreased) and internal
water (increased) but did not affect PPO-water interaction. Consequently,
designed ternary P/T/伪CD supramolecular gels exhibited in situ
gelling at body temperature could host large amounts of PLA/PLGA microspheres
and behaved as porous 3D-scaffolds for mesenchymal stem cells (MSCs)
supporting their osteogenic differentiation. Interestingly, the gels
withstood freeze-drying and reconstitution with minor changes in inner
structure and rheological properties. The gathered information may
help to understand better the supramolecular gels and provide tools
for the rational design of syringeable bone scaffolds that can simultaneously
accommodate cells and drug microcarriers for efficient tissue regeneration