イネ ニオケル コメヌカ タンパクシツ ノ プロテオミクス

Rice bran has wide potential usage as a source of valuable nutrients, though little is known about its protein composition. Here, we identified rice bran proteins by using proteomic analysis. After extraction of crude protein from the bran of Koshihikari brown rice grains, we separated proteins by 2-D PAGE and analyzed 41 protein spots. N-terminal amino acid sequences were determined for 23 spots, 21 proteins of known function and 2 could not be identified by BLAST searching. For the other 18 spots, the N-terminal amino acids could not be determined. Of these, 4 were identified by using LC-MS/MS. After in-solution digestion of bran samples from Koshihikari and TN-1, we looked for differences in the bran proteins between these cultivars. A total of 83 proteins were detected and classified into 10 categories: storage proteins, carbohydrate metabolic enzymes, proteins related to synthesis and proteolysis, embryo proteins, stress-related proteins, regulatory proteins, antioxidant proteins, redox-related proteins, lipid biosynthesis proteins, and energy-related proteins. In the identified proteins, antioxidant proteins and redox-related proteins, such as peroxiredoxin and glyoxalase, respectively, considered to be unique to bran among seed proteins. Such proteins may play important roles as antioxidants for protecting cells in the embryo and aleurone layer from the stress of desiccation

コムギ及び近縁種における多重遺伝子族リブロース-1、5-二燐酸カルボキシラーゼ/オキシゲナーゼ小サブユニット(rbcS)の分子進化学的解析

京都大学0048新制・課程博士博士(農学)甲第7894号農博第1052号新制||農||779(附属図書館)学位論文||H11||N3257(農学部図書室)UT51-99-G488京都大学大学院農学研究科応用生物科学専攻(主査)教授 遠藤 隆, 教授 大西 近江, 教授 佐々木 義之学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA