Photophysicochemical properties and photodynamic therapy activity of highly water-soluble Zn (II) phthalocyanines

The syntheses of two zinc(II) phthalocyanines (ZnPcs) having either imidazole (ZnPc 1) or pyridiloxy (ZnPc 2) moieties as their macrocycle substituents are reported. Quaternization of the ZnPcs with methyl iodide afforded water soluble cationic phthalocyanines. The photophysical, photochemical properties and photodynamic therapy (PDT) activity of the ZnPcs were studied in solution. The fluorescence quantum yield and lifetime of ZnPc 1 were higher as compared to ZnPc 2. ZnPc 2 afforded higher triplet state (ΦT) and singlet oxygen quantum yields (ΦΔ) in comparison to ZnPc 1. The PDT activity of ZnPcs was investigated against human breast adenocarcinoma cells (MCF–7). The two compounds afforded a very minimal in vitro dark cytotoxicity with 85% viable cells at concentration ≤80 μM. On irradiation of the cells having the ZnPcs, ≥50% cell death was recorded for ZnPc 1 which was also evidenced by the cells photo–micrograph

Dual Nuclear/Fluorescence Imaging Potantial of Zinc(II) Phthalocyanine in MIA PaCa-2 Cell Line

WOS: 000390228800005PubMed ID: 27363481Background and Objective: Pancreatic cancer is very common and difficult to diagnose in early stage. Imaging systems for diagnosing cancer have many disadvantages. However, combining different imaging modalities offers synergistic advantages. Optical imaging is the most multidirectional and widely used imaging modality in both clinical practice and research. Methods: In present study, Zinc(II) phthalocyanine [Zn(II) Pc] was synthesized, labeled with iodine-131 and in vitro study was carried out. The intracellular uptake studies of radiolabeled Zn(II) Pc were performed in WI-38 [ATCC CCL-75 (TM), tissue: human fibroblast lung] and MIA PaCa-2 [ATCC CRL-1420 (TM), tissue: human epithelial pancreas carcinoma] cell lines. Results: The intracellular uptake efficiency of radiolabeled Zn(II) Pc in MIA PaCa-2 cells was determined two times higher than WI-38 cells. Also, fluorescence imaging (FI) efficiency of synthesized Zn(II) Pc was investigated in MIA PaCa-2 cells and significant uptake was observed. Conclusion: Zn(II) Pc might be used as a new agent for dual fluorescence/nuclear imaging for pancreatic cancer.Scientific and Technological Research Council of Turkey, TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112T565]The authors gratefully acknowledge financial support by The Scientific and Technological Research Council of Turkey, TUBITAK (Grant no: 112T565)

Effect of Peripheral Substitution on the Performance of Subphthalocyanines in DSSCs

A series of six new subphthalocyanines (SubPcs) bearing an ethynylcarboxyphenyl anchoring unit and decorated with a variety of substituents at the peripheral position of the macrocycle have been synthesized in order to investigate the effect of the peripheral substituent on the performance of dye-sensitized solar cells

Evaluation of photodynamic therapy and nuclear imaging potential of subphthalocyanine integrated TiO2 nanoparticles in mammary and cervical tumor cells

###EgeUn###This study, subphthalocyanines (SubPc) and SubPc integrated TiO2 nanoparticles (SubPc-TiO2) were synthesized as novel photosensitizers. Their PDT effects were evaluated. Furthermore, nuclear imaging potential of I-131-labelled SubPc/SubPc-TiO2 were examined in mouse mammary carcinoma (EMT6) and cervix adenocarcinoma (HeLa) cell lines. The uptake results show that SubPc labelled with I-131 radionuclide ((131) I-SubPc) in EMT6 and HeLa cell lines was found to be approximately the same as in the WI38 cell line. However, the uptake values of SubPc-TiO2 labelled with I-131(I-131-SubPc- TiO2) in EMT6 and HeLa cell lines were determined to be two times higher than in the WI38 cell line. In other words, the target/non-target tissue ratio was identified as two in the EMT6 and HeLa cell lines. I-131-SubPc-TiO2 is promising for imaging or treatment of breast and cervix tumors. In vitro photodynamic therapy studies have shown that SubPc and SubPc-TiO2 are suitable agents for PDT. In addition, SubPc-TiO2 has higher phototoxicity than SubPc. As a future study, in vivo experiments will be held and performed in tumor-bearing nude mice.Scientific and Technological Research Council of Turkey, TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Z430]The authors gratefully acknowledge financial support by The Scientific and Technological Research Council of Turkey, TUBITAK (Grant no: 114Z430)