2 research outputs found
Isomer-Specific Binding Affinity of Perfluorooctanesulfonate (PFOS) and Perfluorooctanoate (PFOA) to Serum Proteins
Perfluorooctanesulfonate (PFOS) and
perfluorooctanoate (PFOA) are
among the most prominent contaminants in human serum, and these were
historically manufactured as technical mixtures of linear and branched
isomers. The isomers display unique pharmacokinetics in humans and
in animal models, but molecular mechanisms underlying isomer-specific
PFOS and PFOA disposition have not previously been studied. Here,
ultrafiltration devices were used to examine (i) the dissociation
constants (<i>K</i><sub>d</sub>) of individual PFOS and
PFOA isomers with human serum albumin (HSA) and (ii) relative binding
affinity of isomers in technical mixtures spiked to whole calf serum
and human serum. Measurement of HSA <i>K</i><sub>d</sub>’s demonstrated that linear PFOS (<i>K</i><sub>d</sub> = 8(±4) × 10<sup>–8</sup> M) was much more tightly
bound than branched PFOS isomers (<i>K</i><sub>d</sub> range
from 8(±1) × 10<sup>–5</sup> M to 4(±2) ×
10<sup>–4</sup> M). Similarly, linear PFOA (<i>K</i><sub>d</sub> = 1(±0.9) × 10<sup>–4</sup> M) was
more strongly bound to HSA compared to branched PFOA isomers (<i>K</i><sub>d</sub> range from 4(±2) × 10<sup>–4</sup> M to 3(±2) × 10<sup>–4</sup> M). The higher binding
affinities of linear PFOS and PFOA to total serum protein were confirmed
when both calf serum and human serum were spiked with technical mixtures.
Overall, these data provide a mechanistic explanation for the longer
biological half-life of PFOS in humans, compared to PFOA, and for
the higher transplacental transfer efficiencies and renal clearance
of branched PFOS and PFOA isomers, compared to the respective linear
isomer
Biomonitoring of Perfluoroalkyl Acids in Human Urine and Estimates of Biological Half-Life
Perfluoroalkyl
acids (PFAAs) are persistent and bioaccumulative
compounds that have been associated with adverse health outcomes.
In human blood, PFAAs exist as both linear and branched isomers, yet
for most linear homologues, and for all branched isomers, elimination
rates are unknown. Paired blood and urine samples (<i>n</i> = 86) were collected from adults in China. They were analyzed by
a sensitive isomer-specific method that permitted the detection of
many PFAAs in human urine for the first time. For all PFAAs except
perfluoroundecanoate (PFUnA), levels in urine correlated positively
with levels in blood. Perfluoroalkyl carboxylates (PFCAs) were excreted
more efficiently than perfluoroalkane sulfonates (PFSAs) of the same
carbon chain-length. In general, shorter PFCAs were excreted more
efficiently than longer ones, but for PFSAs, perfluorooctanesulfonate
(PFOS, a C8 compound) was excreted more efficiently than perfluorohexanesulfonate
(PFHxS, a C6 compound). Among PFOS and perfluorooctanoate (PFOA) isomers,
major branched isomers were more efficiently excreted than the corresponding
linear isomer. A one-compartment model was used to estimate the biological
elimination half-lives of PFAAs. Among all PFAAs, the estimated arithmetic
mean elimination half-lives ranged from 0.5 ± 0.1 years (for
one branched PFOA isomer, 5<i>m</i>-PFOA) to 90 ± 11
years (for one branched PFOS isomer, 1<i>m</i>-PFOS). Urinary
excretion was the major elimination route for short PFCAs (C ≤
8), but for longer PFCAs, PFOS and PFHxS, other routes of excretion
likely contribute to overall elimination. Urinary concentrations are
good biomarkers of the internal dose, and this less invasive strategy
can therefore be used in future epidemiological and biomonitoring
studies. The very long half-lives of long-chain PFCAs, PFHxS, and
PFOS isomers in humans stress the importance of global and domestic
exposure mitigation strategies