5 research outputs found
Airway inflammation, goblet cell hyperplasia and airway fibrosis following long and short term OVA challenges.
<p>Representative photomicrographs of paraffin-embedded lung sections stained with H&E (left column), Alcian-PAS (middle column) and Masson's trichrome (right column) from short term OVA challenged animals (d–l), long term OVA challenged animals (m–u) and after PBS treatment (a–c) 3 days (row 2 and 5), 4 weeks (row 3 and 6) and 8 weeks (row 4 and 7) after challenges. Magnification: 20-fold for H&E, 20-fold for Masson's trichrome, and 40-fold for Alcian-PAS stained sections.</p
Lung function following long and short term airway challenges.
<p>(a) Following OVA sensitisation and short or long term OVA challenges or after sham sensitisation and long or short term PBS challenges, airway responsiveness to MCh was assessed 48 hours after final airway challenge and then weekly for 8 weeks. Mean±SEM of Penh values at 50 mg/ml MCh are shown for the first 4 weeks only from 3 independent experiments (n≥12). No differences between groups were detected after week 4. (b) Baseline Penh values were assessed during the same measurements. Mean±SEM from 5 independent experiments are illustrated (n≥15). Significant differences: * long term OVA challenges versus PBS control, +short term OVA challenges versus PBS control, levels of significance: */+ p<0,01, **/++ p<0,001.</p
Cytokines in BAL fluid after long and short term airway challenges.
<p>Following OVA sensitisation and short or long term OVA challenges or after sham sensitisation and long or short term PBS challenges, concentrations of (a) IL-5, (b) IL-13 and (c) activated TGF-β1were measured in BAL fluid by ELISA 3 days to 8 weeks after final airway challenge, n≥15 per group from 5 independent experiments. Significant differences: * OVA challenges versus PBS controls, +long term versus short term OVA challenges, */+ p<0.05, **/++ p<0.01, ***/+++ p<0.001.</p
Eosinophils and lymphocytes in BAL fluid after long and short term airway challenges.
<p>Following OVA sensitisation and short or long term OVA challenges or after sham sensitisation and long or short term PBS challenges, numbers of (a) eosinophils, and (b) lymphocytes were determined in BAL fluid from 3 days to 8 weeks after final airway challenge, n≥12 per group from 5 independent experiments. Significant differences: * OVA challenges versus PBS controls, +long term versus short term OVA challenges, */+ p<0,05, **/++ p<0,01, ***/+++ p<0,001.</p
OVA specific serum antibodies following sensitisation and short or long term challenges
<p>Serum concentrations of OVA-specific antibodies post challenges were assessed by ELISA. In PBS controls no OVA-specific IgG1 or IgG2a was detected and there was a background of OVA-specific IgE of maximally 1.0 IU/ml. Significant differences: *versus respective PBS control, +versus respective time point after short term OVA challenges, */+ p<0.05, **/++ p<0.01, ***/+++ p<0,001. Means±SEM are shown, n≥9 from 3 independent experiments.</p