The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids

Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test

Embedded control system for autarep - a novel autonomous articulated robotic educational platform

This research introduces an open-source framework, AUTonomous Articulated Robotic Educational Platform (AUTAREP). The platform is centred on a 6 Degree Of Freedom (DOF) arm with multiple feedbacks to ensure precision and autonomy. The sensory system consists of vision, position and force feedbacks while the actuation system comprises six precise DC servo motors. In particular, this paper presents the design of an embedded controller for AUTAREP. The proposed design of the control hardware and software interface has been tailored as per academic requirements of relevant undergraduate and postgraduate courses. Low level commands have been provided to permit readily development of applications for trainees. Advanced users can further exploit the open-source architecture of the platform. The performance of the proposed control system has been demonstrated by various experiments on the fabricated hardware. The control has been subjected to various test inputs to analyse its transient and steady state behaviour. The robot has been tested to achieve a set-point position successfully and the encoder data corresponding to all the joints has been recorded. Finally, a common application of "pick and place" has been implemented. The proposed platform is potentially beneficial in teaching engineering courses, training in industrial sector and research of advanced algorithms

Analysis of COVID-19 burden, epidemiology and mitigation strategies in muslim majority countries

Background: Muslim majority countries have experienced a considerable burden of COVID-19 infection. However, there has been a relative lack of research comparing COVID-19 outbreaks and responses between Muslim-majority countries.Aims: This study aimed to analyse COVID-19 burden, epidemiology and mitigation strategies in Muslim-majority countries.Methods: We use a mixed-methods approach to describe the course of the COVID-19 pandemic throughout the Islamic world, highlight the range of non-pharmaceutical interventions used and the speed with which they were implemented, and investigate reasons behind the differing responses between Muslim-majority countries. The number of cases and deaths per million population, and the mean time taken to implement a range of policies, were compared across the Islamic world. Cases per million population and the mean estimated doubling time for cases was compared between Muslim- majority countries on the basis of governance systems, rapidity of institution of mitigation strategies and conflict groups. We also evaluated pushback to implementation of measures within MMCs, especially from religious quarters.Results: Non-democratic regimes had much shorter doubling time of cases compared to functional democratic Muslim- majority countries (mean 33.9 versus 66.5 days, P = 0.002) and a significantly greater proportion of countries appeared to have flattened the curve by 1 June 2020 (43.8% versus 12.5%, P \u3c 0.03). The doubling time was also significantly greater among countries who implemented lockdown and mitigation measures early (66.7 versus 16.7 days, P \u3c 0.003).Conclusion: Our analysis indicates wide diversity in the COVID-19 response across Muslim majority countries with clear indication that functional democracies were able to contain the epidemic significantly better than nondemocratic regimes. Future analysis should focus on determination of sub-national differentials and risks as well as targeting of interventions

Refining English language tests for university admission: a Malaysian example

English has now become the lingua franca of much of technological, business and academic endeavours. Consequently, learning the English language is now seen as vital, especially at the university level where proficiency in the language has become a selection criterion. At present, the Malaysian University English Test (MUET) has been adopted by Malaysian public universities as an indicator of English language proficiency. A student’s overall result depends on all the four language components of the MUET and often determines the number and nature of the English language courses he or she has to attend at university. This study seeks to examine whether MUET is an accurate predictor of performance and success at university and how the MUET can be finetuned as an entry level English language test. It was carried out among 52 third year undergraduates of the Faculty of Educational Studies, Universiti Putra Malaysia, Serdang, Malaysia, admitted into the Teaching English as a Second Language (TESL) programme. The findings of the study do not offer conclusive evidence about the validity of MUET as a predictor of academic success. However, six models of various combinations of scores on language components on the MUET scores are examined in terms of their effectiveness in increasing the accuracy in selecting students for the TESL programme. The correlations obtained using these models indicate that the combination of various components of the MUET can be used to more accurately predict student achievement at tertiary level than the cumulative MUET score itself. The results of these correlations and their implications in using language tests as admission requirements in general are also discussed

Estimation of the burden of out-of-hospital traumatic cardiac arrest in Karachi, Pakistan, using a cross-sectional capture-recapture analysis

Background: The burden of trauma-related-out-of-hospital cardiac arrest (OHCA) in developing countries like Pakistan remains largely unexplored due to a lack of organized pre-hospital systems. In order to estimate the burden, we used a two-sample capture-recapture method which has been used in several domains to estimate difficult-to-count populations.Methods: We obtained 3-month data from two sources: Records of two major EMS (emergency medical services) systems and five major hospitals providing coverage to the city\u27s population. All adults with traumatic OHCA were included. Information on variables such as name, age, gender, date and time of arrest, cause of arrest, and destination hospital were obtained for these cases and data were compared to obtain a matched sample. Utilizing an equation and different levels of restrictive criteria, estimates were obtained for burden.Results: The EMS records reported 788 and hospital records reported 344 cases of traumatic OHCA. The capture-recapture analysis estimated the annual traumatic OHCA incidence as 45.7/100,000 (95% CI: 44.2 to 47.3). Estimation of the burden from individual hospital or EMS records underestimated and calculated only 14.6% and 33.9% of the total burden, respectively. Most of the traumatic arrest victims had gunshot wound (GSW) (65.2%) followed by road traffic injuries (RTI) (20.8%).Conclusion: The actual burden of traumatic OHCA in Pakistan is larger than the burden reported by either the hospitals or EMS services alone. Most of the cases occurred due to GSW and RTI. A multipronged approach is required to manage the problem; from prevention to developing organized trauma care systems and training lay responders in pre-hospital trauma care is vital

Training English language pre-service teachers using a team based learning approach

Team Based Learning which focuses on enhancing positive group dynamics is a relatively popular instructional approach in several disciplines such as Health Sciences and Business but has yet to gain popularity in Education. This paper examines the use of Team Based Learning in teacher training as well as the receptiveness towards the approach as indicated by a group of Teaching English as a Second Language teacher trainee. The trainees were asked to write diaries regarding their experiences working in a team during a course for a semester which were then collected in three cycles throughout the semester. Entries were analysed in terms of whether there were positive, negative or neutral reference to working in teams. All members of a team were also required to participate in individual micro teaching sessions for which they were evaluated. Findings indicate that Team Based Learning has a potential role in teacher training as positive entries outnumbered negative entries. Additionally, teams with high average micro teaching scores also had more positive diary entries

The utility of modified Butler-Leggett criteria for right ventricular hypertrophy in detection of clinically significant shunt ratio in ostium secundum-type atrial septal defect in adults

Background: This study was performed to test the hypothesis that there exists a correlation between the Butler-Leggett (BL) criterion for right ventricular hypertrophy on the electrocardiogram and the Qp/Qs shunt ratio in adults with ostium secundum atrial septal defects (ASDs). Methods: Demographic, cardiac catheterization, ASD closure, and electrocardiographic data were acquired on 70 patients with secundum ASDs closed percutaneously. Simple linear regression and logistic regression models were created to test the hypothesis. Results: The mean Qp/Qs ratio and BL criterion value were 1.61 +/- 0.46 and 0.11 +/- 0.41, respectively. The BL criterion values correlated with shunt ratios (r(2) = 0.11 and P = .004). A BL criterion value greater than 0 mV predicted a significant shunt ratio (Qp/Qs \u3e or = 1.5) (odds ratio, 4.8; 95% confidence interval, 1.3, 18.1; P = or \u3c.0001) with a sensitivity of 0.68 and specificity of 0.65. Conclusion: Our results indicate that there is limited utility of the BL criterion at detecting right ventricular volume overload, although a BL criterion value greater than 0 mV being used to identify patients with significant intracardiac shunts yielded a sensitivity of 0.68 and specificity of 0.65