EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS

Objective: To evaluate hepatoprotective effects of ethanol extract of aerial part of Blumea lacera (BLEE) against ethanol-induced hepatotoxicity in rats. Methods: The in vivo antioxidant activity of BLEE was assessed by determining the tissue glutathione (GSH) and lipid peroxidation (LPO) levels. The BLEE at the doses of 200 and 400 mg/kg and silymarin 100 mg/kg administered to the ethanol challenged rats. The effects of BLEE and silymarin on Physical and Biochemical Parameters were measured. Similarly, histopathological changes of the liver were studied. Results: The BLEE showed in vivo antioxidant activity. A significant (P<0.001) decrease in SGOT, SGPT, ALP, total and direct bilirubin was observed in BLEE treated group at doses i.e. 200 mg/kg and 400 mg/kg as compared to intoxicated group. Liver damage in animal pretreated with BLEE was minimal with distinct preservation of structures and the architectural frame of the hepatic cells. Conclusion: These findings demonstrated the hepatoprotective effects of BLEE against ethanol-induced liver damage

IN VIVO ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS OF HUGONIA MYSTAX IN PARACETAMOL INDUCED HEPATOTOXICITY IN RATS.

Objectives: The objective of the present work is to study the in vivo anti-oxidant and hepatoprotective effects of Hugonia mystax in paracetamol (PCM)induced hepatotoxicity in rats.Methods: The in vivo anti-oxidant activity of 70% ethanol extract of leaves of H. mystax (HMEE) was assessed by determining the tissue glutathioneand lipid peroxidation (LPO) levels. HMEE 200 and 400 mg/kg p.o. doses and silymarin p.o.100 mg/kg were administered to the PCM challengedrats. The effect of HMEE and silymarin on physical (liver weight and liver volume) and biochemical parameters (serum enzymes [serum glutamicoxaloacetic transaminase (SGOT) and serum glutamate-pyruvate transaminase (SGPT)], alkaline phosphate [ALP], and bilirubin) were measured.Furthermore, histopathological changes in the liver were studied.Results: The HMEE showed in the vivo anti-oxidant activity. Pre-treatment with HMEE for 7 days significantly reduced the elevated biochemicalparameters (SGOT, SGPT, ALP, and bilirubin levels). The hepatic damage in animal pretreated with HMEE was minimal with distinct preservation ofstructures and architectural frame of the hepatic cells.Conclusion: These findings demonstrate the protective nature of HMEE against PCMKeywords: Hugonia mystax, Hepatoprotective, In vivo anti-oxidant, Paracetamol

Antidiarrheal Activity of Albizzia lebbeck Leaves

Worldwide, more than 5–8 million deaths are caused each year by diarrhea. At present, used anti-diarrheal drugs show adverse effects in some form. Hence, there is a need to explore natural drugs as alternative therapy. Preliminary phytochemical screening, Acute oral toxicity and antidiarrheal activity of 70% ethanolic extract of the Albizzia lebbeck leaves (ALL) were performed. ALL were assessed for antidiarrheal activity using castor oil, Prostaglandin-E2 (PGE2) and intestinal motility induced diarrhea in rats. On the basis of acute oral toxicity, 100 and 200 mg/kg body weight doses were selected for antidiarrheal activity. Preliminary phytochemical screenings revealed presence of flavonoids tannins and saponins in extract. ALL showed significant inhibitory activity against castor oil, PGE2 induced diarrhea and intestinal motility. On the basis of the result, it can be concluded that the ALL possess significant antidiarrheal activity

Sphaeranthus indicus Linn.: A Pharmacological Review

Sphaeranthus indicus (SI) Linn. belonging to the family Asteraceae, the whole plant as well as various parts of plant widely used for curing various ailments in the Indian traditional system of medicine. This review focused on review of various pharmacological activities of SI such as anxiolytic, antioxidant, antidiabetic, analgesic, antipyretic, hepatoprotective activity, antimicrobial, antiulcer, anti-inflammatory, neuroleptic, antiviral, ovicidal, anthelmintic activities, and various other activities. Based on vast medicinal use and preclinical trials of this plant, the reported pharmacological activities of SI are covered in this review for exploring the enormous medicinal potential of this plant

Antioxidant and Hepatoprotective activity of Madhuca longifolia (koenig) bark against CCl 4 -induced hepatic injury in rats: In vitro and In vivo studies

ABSTRACT The antioxidant and hepatoprotective effects of 70% ethanolic extract of bark of Madhuca longifolia (koenig) (EEMLK) were studied. The antioxidant property of 70% EEMLK was tested by using reducing power and free radical (hydroxyl and superoxide) scavenging models (invitro); the in-vivo antioxidant activity was assessed by determining the tissue GSH and lipid peroxidation levels. The 70% EEMLK at the doses of 200 and 400 mg/kg and silymarin 100mg/kg were administered to the CCl 4 challenged rats. The effect of 70% EEMLK and silymarin on wet liver weight, liver volume, serum biomarkers like SGOT, SGPT, ALP, direct and total Bilirubin were measured in CCl 4 induced hepatotoxicity in rats. Similarly hepatic tissues were subjected to histopathological observations. The test extract has shown dose dependant antioxidant activity in all the models. The altered biochemical and physical markers by the CCl 4 induced rats brought back to near normal level by the 70% ethanolic extract of MLK in a dose dependent manner