Structure, development, and evolutive patterns of spermatozoa in rhabditid nematodes (Nematoda: Rhabditida)

Spermatogenesis of five rhabditid nematodes was studied using transmission electron microscopy and is described herein. Structure and development of nematode sperm in all available representatives of the extensive order Rhabditida have been analysed and the main characteristics of each infraorder are discussed. The ancestral sperm of the order Rhabditida was reconstructed using maximum likelihood and Bayesian methods based on 44 ultrastructural sperm characters. The hypothetical ancestral spermatogenesis of the order Rhabditida agrees with the previously suggested "rhabditid" pattern and appears to be conserved throughout the order Rhabditida. Despite the enormous variation of rhabditid nematodes, few groups deviate from the ancestral pattern. This conserved pattern can be informative within the phylum Nematoda at order level, but poses limitations when used in taxonomic and phylogenetic analysis within Rhabditida

Structure, development, and evolutive patterns of spermatozoa in rhabditid nematodes (Nematoda: Rhabditida)

Spermatogenesis of five rhabditid nematodes was studied using transmission electron microscopy and is described herein. Structure and development of nematode sperm in all available representatives of the extensive order Rhabditida have been analysed and the main characteristics of each infraorder are discussed. The ancestral sperm of the order Rhabditida was reconstructed using maximum likelihood and Bayesian methods based on 44 ultrastructural sperm characters. The hypothetical ancestral spermatogenesis of the order Rhabditida agrees with the previously suggested "rhabditid" pattern and appears to be conserved throughout the order Rhabditida. Despite the enormous variation of rhabditid nematodes, few groups deviate from the ancestral pattern. This conserved pattern can be informative within the phylum Nematoda at order level, but poses limitations when used in taxonomic and phylogenetic analysis within Rhabditida

EXPERIMENTAL STUDY OF SPECIFIC ACTIVITY OF 1,3-DIAZINON-4 COMPOUND PYaTdl DERIVATIVE IN VIVO

Aim. Study anti-leprosy activity of a 1.3-diazinon-4 compound derivative under the laboratory code PYaTd 1 on the model of intra-plantar infection of mice and evaluate the character of its antibacterial effect. Materials and methods. Study of specific activity was carried out in vivo on the experimental model of leprosy, proposed by Shepard C.C., that assumes execution of intra-plantar infection of mice with a suspension of mycobacteria, produced from lepromas or autopsy tissue of a non-treated leprosy infected, or from tissues of experimental mice, previously infected with Mycobacterium leprae from non-treated patients. The study was carried out on 120 C BA line mice infected with M. leprae (VIII passage) from patient M. Dapsone and PYaTdl compound were administered to animals next day after the infection with forage at a dose of 25 mg/kg for 4.5, 6, 9 and 11 months. The mice were split into 3 groups: control (infected without treatment), comparison (infected, receiving dapsone), experimental (infected, receiving PYaTd 1). After the control term the mice were euthanized under chloroform anesthesia. Suspensions for quantification of mycobacteria were prepared from paw pads. Smears were stained by Ziehl-Nilsson. Results. After 4.5 months the intensity of infect reproduction under the effect of dapsone and PYaTd 1 was reduced compared with control by 18 - 25 times. After a 6-month course - by 50 - 75% and after 9 months - by 85 - 90%. After 11 months in mice that had received PYaTd 1, an intensive suppression of microorganism reproduction was observed: the yield in paws was 70 times lower than in control. In the group that had received dapsone, a reduction of the number of mycobacteria by 20 - 25 times was detected, it was significantly less effective than under the conditions of PYaTd 1 administration. Conclusion. A novel 1.3-diazinon-4 derivative under the code PYaTdl can actively supress reproduction of M. leprae, that gives evidence regarding its specific anti-mycobacterial activity and determines perspectives of its further studies

Using moss Ceratodon purpureus (Hedw.) Brid for assessing the technogenic pollution (Ni, Zn, Mn, Al, Se, Cs, La, and Sm) of transformed ecotopes of Donbass

The ecotopic difference in the accumulation of Ni, Zn, Mn, Al, Se, Cs, La and Sm in bryophyte Ceratodon purpureus (Hedw.) Brid was studied during a long-term experiment in the anthropogenically disturbed environment of Donbass. The concentrations of elements in moss gametophytes were determined by neutron activation analysis. The moss samples were exposed from November 2018 through May 2019 at 24 test plots in the central Donbass with varying degrees of technogenic transformation of geosystems. When comparing ecologically stressed areas with intact or restored ecotopes, the difference in the accumulation of Ni was 6.9 times, Zn – 10.2, Mn – 6.3, Al – 5.3, Se – 9.6, Cs – 3.9, La – 5.9, and Sm – 5.4 times. There were structural and functional modifications in the leaf apparatus of bryophyte, suitable for using in further phytomonitoring studies for express diagnostics. According to the results of the factor analysis, two groups of pollutants were identified, differing in the source of origin: (1) Al, Ni, Mn, and Zn, (2) Cs, Se, La, and Sm. The obtained data are considered as part of the primary screening of biogeochemical characteristics in the Donbass in 2018–2019. Within the gradient of toxic load and local impact, the leading role of mining and metallurgical facilities in the pollution of natural ecosystems of Donbass was proved