Changes in micro-organism abundances in the organic matter released by <i>C</i>. <i>caespitosa</i> during a 96 h incubation.

<p>Prokaryote (black squares) and virus-like-particles (VLPs) abundances (grey circle) were plotted together for T15L40 (a) T15L200 (b) T22L200 (c) and T22L40 (d) condition. Data are mean (n = 9) ± s.e. of three incubations of three different set.</p

Results of the two way analysis of variance with irradiance (L) and temperature (T) as independent variables performed with the R software for net photosynthesis (Pn), respiration (R), total, dissolved and particulate organic carbon (TOC, DOC, POC, respectively), and nitrogen (TON, DON, PON, respectively), the percentage of dissolved organic carbon (%DOC) or nitrogen (%DON), the abundance of prokaryotes and virus-like-particles, the virus to prokaryote ratio (VPR), and the degradation rate of total organic carbon and nitrogen (TOC and TON degradation rate, respectively).

<p>Bold p-value indicated p-value < 0.05 according to the degree of freedom (df) and the ratio between the sum of the mean square of the variance between groups and the sum of the mean square of the variance inside the groups (F-ratio).</p

Fluxes of total (black) dissolved (grey) and particular (white) organic carbon (a) or nitrogen (b) produced by <i>C</i>. <i>caespitosa</i> at 15 or 22°C (T15 and T22 respectively) and 40 or 200 μmol photon m^-2 s^-1 (L40 and L200 respectively).

<p>Data are mean ± s.d. for n = 6 nubbins per condition. Different letters above each bar indicate significant differences.</p

Changes in C. caespitosa physiological parameters at 15 or 22°C (T15 and T22 respectively) and 40 or 200 μmol photon cm^-2 (L40 and L200 respectively) a) protein concentration per skeletal surface area b) chlorophyll concentration per surface area c) chlorophyll per symbiont cell d) symbiont density per surface area e) net photosynthesis (black) and dark respiration (grey) per surface area, and f) calcification rate.

<p>Data are mean (n = 6) ± s.d. (or s.e. for calcification rate), Different letters above each bar indicate significant differences.</p

Organic carbon (a) and nitrogen (b) degradation rates at 15 or 22°C (T15 and T22) and 40 or 200 μmol photon m^-2 s^-1 (L40 and L200 respectively).

<p>Data are mean (n = 6) ± s.d., Different letters above each bar indicate significant differences.</p

Similarity comparison of the bacterial (a) and archaeal (b) diversity between seawater, at 15°C (C15) and 22°C (C22), and the 4 experimental conditions (T15L40, T15L200, T22L40 and T22L200) just after the mucus was released (t₀) and after 96 h of incubation (t₉₆).

<p>The dendrograms were designed using GelCompar II software and they represent the clusters obtained from the 16S rRNA PCR-DGGE analyses.</p

Number of bands observed after PCR DGGE analysis of the bacterial (a) and archaeal (b) community, at 15°C (C15) and 22°C (C22), and in the 4 experimental conditions (T15L40, T15L200, T22L40 and T22L200) just after the mucus was released (black) and after 96 h of incubation (white).

<p>Number of bands observed after PCR DGGE analysis of the bacterial (a) and archaeal (b) community, at 15°C (C15) and 22°C (C22), and in the 4 experimental conditions (T15L40, T15L200, T22L40 and T22L200) just after the mucus was released (black) and after 96 h of incubation (white).</p

Variation in aminopeptidase (a, b) and glucosidase (c, d) activity with mucus aging at 15 or 22°C (T15 and T22 respectively) and 40 or 200 μmol photon m^-2 s^-1 (L40 and L200 respectively).

<p>Data are mean (n = 9) ± s.d. of triplicate analysis of three mucus collected using three different colonies.</p

Variation in prokaryotes (black) and virus-like-particles (VLPs) abundance (grey) at 15 or 22°C (T15 and T22 respectively) and 40 or 200 μmol photon m^-2 s^-1 (L40 and L200 respectively).

<p>Data are mean (n = 9) ± s.d. of triplicate analysis of three mucus collected on each three paired-nubbins. Different letters above each bar indicate significant differences.</p