Experimental Simulation of the Radionuclide Behaviour in the Process of Creating Additional Safety Barriers in Solid Radioactive Waste Repositories Containing Irradiated Graphite

Results of the experimental modeling of radionuclide behavior when creating additional safety barriers in solid radioactive waste repositories are presented. The experiments were run on the repository mockup containing solid radioactive waste fragments including irradiated graphite. The repository mockup layout is given; the processes with radionuclides that occur during the barrier creation with a clayey solution and during the following barrier operation are investigated. The results obtained confirm high anti-migration and anti-filtration properties of clay used for the barrier creation even under the long-term excessive water saturation of rocks confining the repository

The clinical case of acute promyelocytic leukemia in a patient with high cytogenetic risk

The article presents an overview of the clinical case of acute promyelocytic leukemia in a patient with high cytogenetic risk. Key methods for laboratory diagnosis of this disease are indicated.В статье представлен обзор клинического случая острого промиелоцитарного лейкоза у пациента с высоким цитогенетическим риском. Указаны основные методы лабораторной диагностики данного заболевани

Clinical and diagnostic significance of some laboratory analysis of pericardial fluid

The aim of the study – identify differential laboratory characteristic of pericardial fluid examination in exudative and metastatic pericarditis.Цель исследования – установить дифференциальные лабораторные признаки исследования ПЖ при воспалительном и метастатическом перикардите

Microvesicles produced by natural killer cells of the NK-92 cell line affect the phenotype and functions of endothelial cells of the EA.Hy926 cell line

Microvesicles (MVs) are small (100-1000 nm) subcellular structures produced by both motionless and activated cells that can transfer molecules to target cells, and regulate physiological and pathological processes. MVs of leukocyte origin, in particular those produced by natural killer cells (NK cells), remain the least studied population of MVs. NK cells can change the functional activity of endothelial cells (ECs) and are involved in regulating angiogenesis. The ability of NK cell-derived MVs to influence the functionality of ECs is understudied currently. We aimed to study the effect of MVs produced by NK cells of the NK-92 cell line on the phenotype, caspase activity, proliferation and migration of ECs of the EA.Hy926 cell line. We cultured ECs in the presence of MVs derived from the NK-92 cell line, and then used flow cytometry to assess changes in EC phenotype, intracellular protein transfer from MVs to ECs, and the relative death of ECs. We used western blot analysis to evaluate the expression of granzyme B in NK cells and in the MVs that they produced, as well as the expression of granzyme B, caspases, extracellular-regulated kinase (ERK) and protein kinase B (AKT) in ECs. We also assessed the proliferation and migration of ECs in the presence of MVs derived from cells of the NK-92 cell line. The results revealed significant differences in the proteomic profiles of cells of the NK-92 cell line and their MV product. Contact between ECs and MVs derived from cells of the NK-92 cell line is accompanied by the following events: a) expression of granzyme B in ECs, b) activation of caspase-9 and caspase-3, with partial EC death, c) appearance of the panleukocyte marker CD45 on ECs, d) decrease in CD105 expression, and increase in CD34 and CD54 expression, and e) inhibition of EC migration. Transfer of ERK (but not AKT) from MVs derived from cells of the NK-92 cell line to ECs, at a concentration 10 times lower than that which causes EC death, leads to an increase in EC proliferation

Reducing high risk of cardiovascular events in Type 2 diabetes mellitus: DIALOG Study results

Aim. To study the real-world pharmaceutical reduction of high cardiovascular disease (CVD) risk in patients with Type 2 diabetes mellitus (DM-2).Material and methods. In total, 200 DM-2 patients agreed to participate in the study, including survey, anthropometry, and laboratory tests.Results. In two-thirds of the patients, carbohydrate metabolism was decompensated due to inadequate disease awareness, inadequate or absent glycemia self-control, and rare prescription of combined therapy. The main reasons for not achieving target blood pressure (BP) levels included high rates of monotherapy, low doses of antihypertensive drugs, inadequate treatment compliance, and low rates of BP self-control. Statins were taken by 23% of the patients, and aspirin medications – by 16%.Conclusion. In real-world clinical settings, a substantial proportion of DM-2 patients require intensified treatment, in accordance with the concept of multi-factorial risk reduction

ANOTHER LOOK AT TYPE 2 DIABETES PATIENT: “DIALOGUE” STUDY RESULTS

Aim. To evaluate factors affecting hypoglycemic therapy in patients with type 2 diabetes mellitus (DM2) in real clinical practice.Material and methods. 200 patients with DM2, who consented to take part in the questionnaire, were examined. Questionnaire data evaluation and clinical examination was performed as well as plasma levels of glucose, glycosylated hemoglobin (HbA1c) and lipids estimation.Results. Low rate of diagnosed DM2 complications especially nephropathy was observed. 68% of patients had high level of glycosylated hemoglobin (HbA1c) because of their knowledge lack as well as inadequate glucose self-monitoring or it’s absent and uncommon combined glucose-lowering therapy. Depression was revealed in 43,5% of patients, especially in patients with chronic DM2 complications. Low incidence of depression was associated with good patient knowledge about DM2. Well informed patients had lower score according to CES-D scale than patients with DM2 complications.Conclusion. Appropriate drug therapy, patients’ education, early depression diagnosis and treatment are necessary to improve glycemic control in DM2 patients.</p

ANOTHER LOOK AT TYPE 2 DIABETES PATIENT: “DIALOGUE” STUDY RESULTS

Aim. To evaluate factors affecting hypoglycemic therapy in patients with type 2 diabetes mellitus (DM2) in real clinical practice.Material and methods. 200 patients with DM2, who consented to take part in the questionnaire, were examined. Questionnaire data evaluation and clinical examination was performed as well as plasma levels of glucose, glycosylated hemoglobin (HbA1c) and lipids estimation.Results. Low rate of diagnosed DM2 complications especially nephropathy was observed. 68% of patients had high level of glycosylated hemoglobin (HbA1c) because of their knowledge lack as well as inadequate glucose self-monitoring or it’s absent and uncommon combined glucose-lowering therapy. Depression was revealed in 43,5% of patients, especially in patients with chronic DM2 complications. Low incidence of depression was associated with good patient knowledge about DM2. Well informed patients had lower score according to CES-D scale than patients with DM2 complications.Conclusion. Appropriate drug therapy, patients’ education, early depression diagnosis and treatment are necessary to improve glycemic control in DM2 patients

Main elements of taxation in the conditions of the development of digital economy

The purpose of this article is to consider the main elements of taxation in a digital economy. On the base of the analysis, it was concluded that in conditions of digital economy, the development of information resources would provide a concretization of the provisions of the main elements of taxation and optimize the emergence and pursuance of the constitutional duties to pay taxes, as well as the calculation of tax amounts. The main provisions and conclusions can be used in scientific and practical activities when considering the issues of the legal construction of taxes.El propósito de este artículo es la consideración de los principales elementos de los impuestos en una economía digital. Sobre la base del análisis del estudio, se concluyó que, en condiciones de economía digital, el desarrollo de los recursos de información proporciona una concreción de las disposiciones de los principales elementos de la tributación y optimiza la aparición y el cumplimiento de los deberes constitucionales de pagar impuestos., así como el cálculo de los importes de impuestos. Las principales disposiciones y conclusiones pueden utilizarse en actividades científicas y prácticas al considerar los problemas de la construcción legal de impuestos