Additional file 2: Figure S1. of Monocyte unresponsiveness and impaired IL1β, TNFι and IL7 production are associated with a poor outcome in Malawian adults with pulmonary tuberculosis

Gating strategy to identify monocyte populations. The first plot represents the forward scatter- side scatter characteristics and the second the separation according to CD14 and CD16 staining. (JPEG 61 kb

Additional file 1: Table S1. of Monocyte unresponsiveness and impaired IL1β, TNFι and IL7 production are associated with a poor outcome in Malawian adults with pulmonary tuberculosis

Median and IQR for responses to stimulation with heat killed Mycobacterium Tuberculosis (H37Rv) or Lipopolysaccaride (LPS). P-values are derived from Mann-Whitney U tests. 11 analyses were performed for each stimulus, giving a corrected p value of 0.0045 using the Bonferroni correction. (DOCX 17 kb

Additional file 11: of Understanding pneumococcal serotype 1 biology through population genomic analysis

Distribution of genes associated with virulence and colonisation in the ST217 in serotype 1 isolates. Presence of the genes was screened in all the isolates using BLAST as described in the methods section. (PDF 1702 kb

Additional file 13: of Understanding pneumococcal serotype 1 biology through population genomic analysis

Distribution of the accessory genes and antibiotic resistance conferring elements in the ST217 isolates. a) Maximum likelihood phylogeny of the isolates rooted using isolates from ST615 as an outgroup (not shown). b) Number of shared accessory open reading frames (ORFs) or genes between pairs of isolates in the phylogeny on the left side and at the top. c) Colour strips showing the continent and country of origin of the isolates and their SCs. d) A heatmap showing the number of shared accessory genes between each pair of isolates in the phylogeny (panel [a] and [e]). (PDF 4076 kb