Designing an Integrated Environment for Artificial Intelligence

The SHELLEY RESEARCH GROUP (part of the Illinois Wesleyan Intelligence Network on Knowledge -IWINK) has been in existence for several years, and has benefited immensely from various student contributors who have added such components as robotic arm control, cross platform networking, an artificially intelligent tic-tac-toe player, and an interactive teaching tool demonstrating the functionality of artificial neural networks. What is lacking, however, amidst these undergraduate contributions to the SHELLEY Project, is an effective means of integrating existing components into a single cohesive functional unit, let alone any easy means of making further contributions within a simple unified context. The focus of this research has been to design an all-encompassing structure for incorporating the different components of SHELLEY (both existing and future). Because we must operate under the assumption that we cannot predict what future contributions will be made to SHELLEY, nor how these components will be used, this integrated environment must be both flexible and expandable in such a way as to not confine future projects. The approach to artificial intelligence that the SHELLEY RESEARCH GROUP has taken relies heavily upon interaction with the surrounding environment. For this reason, many of the existing components are devices for receiving input from SHELLEY\u27S surroundings (such as vision cameras) or acting upon the surroundings (such as robotic arms). Thus, we can assume that future contributions will fall under two primary categories: additional devices (either cognitive, modules, such as neural networks, or interactive devices, such as cameras or arms), or intelligent agents (such as tic-tac-toe players, or navigation systems) that will use these devices. The environment must then be flexible in two manners -allowing for the addition of further devices, and providing a task management mechanism for accessing these devices. The solution is to use a modern operating system model where the devices that SHELLEY uses to interact with her environment correspond to computer hardware devices and their drivers, the intelligent agents are analogous to processes that run on the system and use the devices, and the administrator, which coordinates these agents and their usage of devices, can be compared to the kernel of the modern operating system

Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. Several functions have been proposed for the Escherichia coli DNA polymerase IV (pol IV). Although much research has focused on a potential role for pol IV in assisting pol III replisomes in the bypass of lesions, pol IV is rarely found at the replication fork in vivo. Pol IV is expressed at increased levels in E. coli cells exposed to exogenous DNA damaging agents, including many commonly used antibiotics. Here we present live-cell single-molecule microscopy measurements indicating that double-strand breaks induced by antibiotics strongly stimulate pol IV activity. Exposure to the antibiotics ciprofloxacin and trimethoprim leads to the formation of double strand breaks in E. coli cells. RecA and pol IV foci increase after treatment and exhibit strong colocalization. The induction of the SOS response, the appearance of RecA foci, the appearance of pol IV foci and RecA-pol IV colocalization are all dependent on RecB function. The positioning of pol IV foci likely reflects a physical interaction with the RecA* nucleoprotein filaments that has been detected previously in vitro. Our observations provide an in vivo substantiation of a direct role for pol IV in double strand break repair in cells treated with double strand break-inducing antibiotics

Vitamin B-12 release from P(HEMA-co-THFMA) in water and SBF: A model drug release study

A model drug release study on the ingress of water and Kokubo simulated body fluid (SBF) into poly(2-hydroxyethyl methacrylate) (THFMA) and its copolymers with tetrahydrofurfuryl methacrylate (THFMA) loaded with vitamin B-12 was undertaken over the temperature range 298-318 K. The polymers were studied as cylinders and were loaded with either 5 or 10 wt-% of the drug. The drug release from the polymers was found to follow a Fickian diffusion mechanism in the early stages of the drug release, with higher normalized release rates at higher temperatures and higher drug loadings. The normalized release rates were also found to be higher for the SBF solution than for water. The copolymer composition was found to have a significant effect on the rate of release of the drug, with the rate falling rapidly between HEMA mole fractions of 1.0 and 0.8, but for lower mole fractions of HEMA the normalized release rate decreased more slowly. This behaviour followed the trend found for the changes in the equilibrium penetrant contents for the copolymers

Clinical decision support tools for paediatric sepsis in resource-poor settings: an international qualitative study

Objective New paediatric sepsis criteria are being developed by an international task force. However, it remains unknown what type of clinical decision support (CDS) tools will be most useful for dissemination of those criteria in resource-poor settings. We sought to design effective CDS tools by identifying the paediatric sepsis-related decisional needs of multidisciplinary clinicians and health system administrators in resource-poor settings.Design Semistructured qualitative focus groups and interviews with 35 clinicians (8 nurses, 27 physicians) and 5 administrators at health systems that regularly provide care for children with sepsis, April–May 2022.Setting Health systems in Africa, Asia and Latin America, where sepsis has a large impact on child health and healthcare resources may be limited.Participants Participants had a mean age of 45 years, a mean of 15 years of experience, and were 45% female.Results Emergent themes were related to the decisional needs of clinicians caring for children with sepsis and to the needs of health system administrators as they make decisions about which CDS tools to implement. Themes included variation across regions and institutions in infectious aetiologies of sepsis and available clinical resources, the need for CDS tools to be flexible and customisable in order for implementation to be successful, and proposed features and format of an ideal paediatric sepsis CDS tool.Conclusion Findings from this study will directly contribute to the design and implementation of CDS tools to increase the uptake and impact of the new paediatric sepsis criteria in resource-poor settings