Media 1: Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution

Originally published in Biomedical Optics Express on 01 December 2014 (boe-5-12-4235

The distal end of the OR-PAT catheter.

<p>(a) Overall architecture. (b) Side and (c) Top view of the catheter under a conventional optical microscope. FSC, flexible stainless steel coil; PT1, polyimide tube 1 with an inner diameter of 0.7 mm; PT2, polyimide tube 2 with an inner diameter of 0.74 mm; STH, stainless steel tube housing; UST, ultrasonic transducer; SMF, single-mode fiber; TC, transducer cable; GL, gradient-index lens; M, micro-prism; B, metal base.</p

Supplementary document for In-vivo assessment of rat rectal tumor using optical-resolution photoacoustic endoscopy - 6877424.pdf

supplementary materia

Photoacoustic and ultrasonic imaging with OR-PAT of a stent deployed in a plastic tube.

<p>Three-dimensional cut-away (a) photoacoustic and (d) ultrasonic images. Representative (b) photoacoustic and (e) ultrasonic B-scans. Enlarged photoacoustic (c) and ultrasonic images corresponding to the dash boxes in (b) and (e).</p

The spatial resolutions of photoacoustic and ultrasonic imaging of OR-PAT.

<p>(a) Photoacoustic B-scan image of a carbon fiber (6 μm in diameter). (b) Enlarged view of the dashed box in (a). (c) Ultrasonic B-scan image of a tungsten wire (12 μm in diameter). (d) Enlarged view of the dashed box in (c). (e) The transverse and (f) axial resolutions of photoacoustic (red) and ultrasonic (blue) imaging with OR-PAT. (g) The spatial resolutions and (h) SNR as a function of the axial position. PA, photoacoustics; US, ultrasonics; Trans., transverse; PSF, point spread function.</p

Illustration of the OR-PAT system.

<p>(a) Overall architecture of the system. (b) Schematic of the rotational mechanism of the catheter. (c) A photo of the catheter showing its flexibility. OPO, optical parametric oscillator; NDF, neutral density filter; ConL, condenser lens; BS: beam splitter; PD, photodiode; FC, fiber coupler; US, ultrasonics; PA, photoacoustics; SMF, single mode fiber; EC, electrical cable; 3D scanner, consisting of an optical-electric rotary joint (ROJ), a step motor, and a motorized pull-back stage.</p

Spectroscopic OR-PAT of lipid.

<p>(a)–(d) Photoacoustic images at four different laser wavelengths of a phantom made by placing a small amount of butter into chicken breast tissue (see inset in (e)). The yellow box in (a) corresponds to the butter content in (e), while the white box in (d) indicates the area that photoacoustic signals are averaged for the photoacoustic spectra plot in (e). (e) Comparison between the acquired photoacoustic spectra of butter and previously reported optical absorption spectra of lipid <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092463#pone.0092463-Tsai1" target="_blank">[29]</a>.</p

OR-PAT of an iliac/common femoral artery stent.

<p>(a) Optical microscopic image of the imaged sent segment. (b) Representative 3D photoacoustic and (c) ultrasonic images. (d) Photoacoustic, (e) ultrasonic, and (f) fused B-scan images of a cross section of the stent. (g) Axial and (h) transverse photoacoustic (red) and ultrasonic (blue) signal profiles of a wire junction at 2 o′clock in (f). PA, photoacoustics; US, ultrasonics; Trans., transverse.</p

Visualization 2.avi

SLN needle aspiration biopsy process based on the guidance of real-time photoacoustic imagin