Absence of a long-term trend in <i>s</i>_{<i>20T</i>,<i>t</i>,<i>r</i>,<i>v</i>}-values of the BSA monomer with time of experiment for the three kits (blue, green, and magenta).

<p>Highlighted as bold solid line is the overall average, and the grey area indicates one standard deviation.</p

Corrected best-fit apparent monomer molecular mass from integration of the <i>c</i>(<i>s</i>) peak when scanned with the absorbance system (green) and the interference system (magenta).

<p>Only data with rmsd less than 0.01 OD or 0.01 fringes were included. The box-and-whisker plot indicates the central 50% of the data as solid line and draws the smaller and larger 25% percentiles as individual circles. The median is displayed as a vertical line.</p

Examples of transient changes in the console temperature reading during the SV experiment, as saved in the scan file data.

<p>For comparison, the maximum adiabatic cooling of -0.3°C would be expected after approximately 300 sec, recovering to the equilibrium temperature after approximately 1,200 s (see Fig 3 in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126420#pone.0126420.ref033" target="_blank">33</a>]).</p

Histogram and box-and-whisker plot of <i>s</i>-values of the BSA monomer after different corrections: Raw experimental <i>s</i>-values (black, with grey histogram), scan time corrected <i>s</i>_<i>t</i>-values (blue), rotor temperature corrected <i>s</i>_<i>20T</i>-values (green), or radial magnification corrected <i>s</i>_<i>r</i>-values (cyan), and fully corrected <i>s</i>_{<i>20T</i>,<i>t</i>,<i>r</i>,<i>v</i>}-values (red with red histogram).

<p>The box-and-whisker plots indicate the central 50% of the data as solid line and draw the smaller and larger 25% percentiles as individual circles. The median for each group is displayed as a vertical line.</p

Observed fraction of dimer (as a ratio of dimer peak area to the sum of monomer plus dimer peak areas).

<p>The box-and-whisker plot indicates the central 50% of the data as solid line and draws the smaller and larger 25% percentiles as individual circles. The median displayed as vertical line. The mean and standard deviations are 18.5% ± 1.1% for the absorbance system, and 19.0% ± 2.1% for the interference system.</p

Example for the analysis of absorbance data from the sedimentation velocity experiment of BSA.

<p>(A) Absorbance scans (symbols) and best-fit <i>c</i>(<i>s</i>) model at different points in time indicated by color temperature. (B and C) Bitmap and overlay of the residuals of the fit. (D) <i>c</i>(<i>s</i>) sedimentation coefficient distribution showing peaks for monomer, dimer, trimer, and traces of higher oligomers.</p

Magnitude of the radial magnification correction obtained with the absorbance system (green) and the interference system (magenta).

<p>The box-and-whisker plot above the histogram indicates the central 50% of the data as solid horizontal line and draws data in the smallest and highest 25% percentiles as individual circles. The median is displayed as vertical line. The mean and standard deviations are -0.43% ±1.36% for the absorbance system, and -0.75% ± 0.82% for the interference system (once the three outliers are excluded).</p

Correlation between the ratio of uncorrected <i>s</i>-values for the BSA monomer peak from the simultaneously acquired interference and the absorbance data (<i>s</i>_IF/<i>s</i>_ABS) and the ratio of corresponding radial magnification correction factors measured independently with the steel mask (r_IF/r_ABS).

<p>Squares are a histogram with frequency as indicated in the sidebar. The dotted line indicates the ideally expected relationship assuming perfect measurements of <i>s</i>-values and radial magnification correction factors.</p