Additional file 1: of General and disease-specific pain trajectories as predictors of social and political outcomes in arthritis and cancer

Table S1. Baseline health and socio-demographic characteristics of the sample. Comparisons are between the cancer and arthritis subsamples. Table S2. Binary logistic regression models modelling the relationship between pain and dropout or death in the Wave 1 ELSA respondents and the cancer and arthritis subsamples. Table S3. Model characteristics of each identified trajectory. Table S4. Uncorrected post hoc 2 × 2 χ2 tests on dropout by Wave 7 between classes. Table S5. Growth models for social and civic engagement variables (unstandardised). Table S6. Regression coefficients for age and trajectory on the intercept and slope for growth models for each index of social and civic engagement for respondents in the whole sample. Table S7. Regression coefficients for age and trajectory on the intercept and slope for growth models for each index of social and civic engagement for respondents with arthritis. Table S8. Regression coefficients for age and trajectory on the intercept and slope for growth models for each index of social and civic engagement for respondents with cancer. Figure S1. Histogram of the chronic pain measurement at Wave 1 for all Wave 1 ELSA respondents. Figure S2. Histogram of the chronic pain measurement at Wave 1 for respondents included in the arthritis subsample. Figure S3. Histogram of the chronic pain measurement at Wave 1 for respondents included in the cancer subsample. (DOCX 3796 kb

Percentage quenching of nuclease Trp fluorescence by brominated lipids.

<p>Nucleases were incubated with LUVs at P∶L of 1∶150 in 10 mM Kpi pH 7.5. Standard errors are derived from the averaging of a minimum of three independent experiments. N.Q. denotes no quenching.</p

Equilibrium binding constants and Hill coefficients for the association of nuclease domains with synthetic or <i>E. coli</i> lipid LUVs.

<p>Incubations were performed in 10 mM Kpi, pH 7.5, at 25°C, with a lipid concentration of 200 µM. Standard errors are derived from the non-linear regression analysis. N.A. indicates absence of cooperativity. Nuclease titrations were carried out at least twice, with similar results.</p

Vesicle leakage as a result of nuclease membrane binding.

<p>Nuclease colicins were added to SRB-containing LUVs; DOPC∶DOPG (3∶1) at P∶L of 0.02 (solid black bars) or 0.002 (solid white bars) and <i>E. coli</i> lipid at P∶L 0.02 only (striped bars). The SRB release after 5 min is presented as a percentage of total release (after Triton X-100 addition). The inset shows the effect of increasing nuclease (E7 (◊) and E3 (Δ)) concentration on the SRB release from DOPC∶DOPG (3∶1) LUVs. The standard errors are derived from the averaging of a minimum of three independent experiments.</p

Extent of lipid mixing induced by nuclease membrane binding.

<p>Nuclease colicins were added at P∶L of 0.02 (black bars) and 0.002 (grey bars) to DOPC∶DOPG (3∶1, solid bars) or <i>E. coli</i> lipid (striped bars) LUVs. Lipid mixing was deduced from the loss of FRET efficiency after addition of the nucleases to a LUVs suspension containing NBD-PE- and Rh-PE-labelled (0.6 mol % each) LUVs with a four-fold excess of unlabelled LUVs. The standard errors are derived from the averaging of a minimum of three independent experiments.</p

Effect of nuclease addition on LUV aggregation.

<p>Nuclease domains were added to a LUV suspension (100 µM lipid concentration) at a range of P∶L and the aggregation was monitored via measuring the OD at 436 nm. Nuclease addition to DOPC∶DOPG (3∶1) and <i>E. coli</i> lipid LUVs are depicted by closed and open symbols respectively. DNases: E7 (◊) and E9 (o), rRNase E3 (Δ). The DNases JB10 and E8 gave similar results to E9 and are omitted for clarity. The standard errors are derived from the averaging of a minimum of three independent experiments.</p

Nuclease colicins used in this work.

a<p>DNase JB10 is an E9-based construct with two Lys residues (at positions 21 and 45) mutated to Glu <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046656#pone.0046656-Walker1" target="_blank">[17]</a>.</p>b<p>Hydrophobicity is calculated according to Hessa <i>et al</i>. where smaller values indicate larger hydrophobicity <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046656#pone.0046656-Hessa1" target="_blank">[28]</a>.</p

Stability of the calf social network during barn grouping.

<p>Stability of the calf social network during barn grouping.</p

Social heterogeneity of calves, measured at the group level, during barn grouping.

<p>Social heterogeneity of calves, measured at the group level, during barn grouping.</p

Vocal responses of calves to weaning.

<p>The total number of vocalisations, during one hour observations of calves, over each three-day period (pre-weaning, weaning and post-weaning).</p