Phase separation in thermoplastic polyurethane elastomers: a structure–property investigation

The effect of soft segment, isocyanate and chain extender chemical structure on the physical and mechanical properties of thermoplastic polyurethane elastomers was systematically investigated. Several series of materials were produced with identical stoichiometry and under the same reaction conditions. The raw materials used to synthesise these materials were systematically altered to facilitate comment on the influence of molecular weight, cohesive energy density (CED) and molecular geometry on the phase separation phenomena observed in polyurethane elastomers. [Continues.

Phylogenetic relatedness of OHSU_I, OHSU_II and OHSU_III microbes among different <i>Bradyrhizobiaceae</i> species.

<p>Phylogenetic analysis based on 16S rRNA gene sequences (A) and whole rRNA operon sequences (B) using the neighbor-joining method. GenBank Accession numbers of sequences used in the analyses are shown in parentheses. Scale bar units are estimated branch lengths. Numerals indicate bootstrap percentages over 50 after 500 replications.</p

Sequence mapping for draft genomes of <i>A. septicemium</i> grown in OHSU_II and OHSU_I cultures and genome of <i>A. broomeae.</i>

<p>The complete genome of <i>Bradyrhizobiaceae</i> SG-6C was used as the reference to align genomic scaffold supercontigs 1.2, 1.3 and 1.1 of <i>A. broomeae</i> into a working complete genome that was then used as the reference genome for assembling formed contigs from genomic sequencing of the microbes grown in OHSU_I and OHSU_II cultures into draft genomes. The tracks from inside to outside represent GC skews, GC contents, draft genome of OHSU_II microbe, draft genome of OHSU_I microbe and <i>A. broomeae</i> genome. The color blank regions represent sequence differences found between the bacterial genomes. Major regions of difference are seen in the regions of 0.6 Mb and 3 Mb.</p

Photomicrographs of OHSU_I, OHSU_II and OHSU_III microbes.

<p><b>A:</b> Photomicrograph of OHSU_II C1 forming microscopic colonies on the surface of a YM agar plate after 2 weeks of incubation at 30°C. The streaked lines on the agar plate could be clearly seen. 40X magnification. <b>B and C:</b> Photomicrographs of OHSU_I C6 and OHSU_II C1 microbes growing at high cell density in the culture broths kept at RT after more than 10 days. The microbes stayed at the bottom of undisturbed culture flasks. Many of the OHSU_I C6 microbes aggregated into clumps and adhered on the plastic flask surface (B). OHSU_II C1 did not aggregate into clumps or adhere on the plastic surface (C). Phase contrast with green filter 400X. <b>D:</b> Photomicrograph of OHSU_III culture. The microbes in the broth appeared to increase in numbers and form loose aggregations (empty arrows) seen at the bottom of the culture flask. However, the microbes could not grow and reach into a high cell density after 3 months of incubation at RT. Fragments of potential blood cells debris were also seen (arrows). Phase contrast 400X</p

Whole genome sequencing datasheet of new <i>Bradyrhizobiaceae</i> from 3 blood samples.

<p>Whole genome sequencing datasheet of new <i>Bradyrhizobiaceae</i> from 3 blood samples.</p

Comparison of growth characteristics, biochemical reactions and G/C contents of <i>Afipia</i> species.

†<p><i>Afipia</i> species and related strains or isolates: 1, OHSU_I uncloned; 2, OHSU_I-C4; 3, OHSU_I-C6; 4, OHSU_II uncloned; 5, OHSU_II-C1; 6, OHSU_II C2; 7, A. felis; 8, <i>A. felis</i> genospecies; 9-12, <i>Afipia</i> genospecies (1 to 4); 13, <i>A. clevelandensis</i>; 14, <i>A. broomeae</i> (3 strains); 15, <i>A. birgiae</i>; 16, <i>A. massiliensis</i>. Data for <i>A. broomeae</i>, <i>A. clevelandensis</i>, <i>A. felis</i>, <i>A. felis</i> genospecies, 4 <i>Afpia</i> genospecies, <i>A. birgiae</i> and <i>A. massiliensis</i> are from Brenner et al. (15) and La Scola et al. (9).</p>‡<p>Bacteria were grown on YM agar before use of biochemical studies, except noted by a* for grown on BCYE agar.</p><p>P: positive, p: weak positive, N: negative.</p

Alignment of whole-genome sequences using draft genomes of OHSU_I isolates (un-cloned, C4 and C6) and OHSU_II isolates (un-cloned, C1 and C2).

<p>Homologous locally collinear blocks among genomes are connected with a line and identified by the same color using progressiveMauve. Blocks that are inverted compared to the OHSU_I (un-cloned) genome are placed under the center line of the genome. Gene rearrangements or inversions among the genomes are indicated. The genome content dissimilarities among these isolates were calculated and are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076142#pone-0076142-t003" target="_blank">Table 3</a>.</p

Sequence mapping for draft genomes of <i>Bradyrhizobium</i> sp. OHSU_III and the genomes of <i>B. elkanii</i> and <i>B. japonicum.</i>

<p>The tracks from inside to outside represent GC skews, GC contents, draft genome of OHSU_III microbe, draft genome <i>B. elkanii</i> and complete genome of <i>B. japonicum.</i>. The color blank regions represent areas of sequence differences found between the bacterial genomes.</p

Genome content differences among OHSU_I, OHSU_II isolates of <i>A. septicemium</i>, established <i>Afipia</i> species and <i>Bradyrhizobiaceae</i> SG.

<p>Rows 1-12 correspond to columns 1-12.</p

Additional file 1: Table S1. of Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer

Patient and tumor characteristics of PCa patients (n = 170). (DOCX 13 kb