Metabolomics Reveals Novel Serum Metabolic Signatures in Gastric Cancer by a Mass Spectrometry Platform

Gastric cancer (GAS) is one of the malignant tumors of the gastrointestinal system. Alterations in metabolite composition can reflect pathological processes of GAS and constitute a basis for diagnosis and treatment improvements. In this study, a total of 301 serum samples from 150 GAS patients at different tumor–node–metastasis (TNM) stages and 151 healthy controls were collected. Mass spectrometry platforms were performed to investigate the changes in GAS-related metabolites and explore the new potential serum biomarkers and the metabolic dysregulation associated with GAS progression. Twelve differential metabolites (ethyl 2,4-dimethyl-1,3-dioxolane-2-acetate, D-urobilinogen, 14-HDoHE, 13-hydroxy-9-methoxy-10-oxo-11-octadecenoic acid, 5,6-dihydroxyprostaglandin F1a, 9′-carboxy-gamma-tocotrienol, glutaric acid, alanine, tyrosine, C18:2(FFA), adipic acid, and suberic acid) were identified to establish the diagnosis model for GAS. The defined biomarker panel was also statistically significant for GAS progression with different TNM stages. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment revealed the metabolic dysregulation associated with GAS progression. In conclusion, a diagnostic panel was established and validated, which could be used to further stage the early and advanced GAS patients from healthy controls. These findings may provide useful information for explaining the GAS metabolic alterations and try to facilitate the characterization of GAS patients in the early stage

Effects of mangiferin on the proteins expression of FFA metabolism including AMPK, CD36, CPT1, ACC and DGAT2 in liver of hyperlipidemic rats.

<p>Wistar rats were divided randomly into five groups (n = 10 per group): control group (fed an AIN-93G diet); hyperlipidemia group (fed a high-fat diet); mangiferin-supplemented groups, fed the high-fat diet and different doses of mangiferin (50, 100, 150 mg/kg BW/d). The experiment lasted for 6 weeks, and the liver was taken for western blot analysis. (A) AMPK phosphorylation level. (B) CD36 expression on cell membrane. (C) CPT1 expression in mitochondrion. (D) ACC level and activity. (E) DGAT2 expression. * P<0.05 compared with hyperlipidemic group.</p

Effects of compound C on mangiferin induced FFA uptake, intracellular FFA, TG and FFA metabolism proteins expression in HepG2 cells.

<p>HepG2 cells were pretreated 1 h with compound C, an AMPK inhibitor, and then treated with 100 µmol/L mangiferin and 0.2 mmol/L OA for 24 h. OA in medium (To assess the uptake of OA) (A), intracellular OA (A), intracellular TG (A) and proteins expression of FFA metabolism including AMPK (B), ACC (B), CD36 (B), CPT1 (B) and DGAT2 (B) were determined by western blot method. The experiments were repeated 3 times. Data are presented as means ± SD (n = 3). ^*<i>P</i><0.05 and ^**<i>P</i><0.01 compared with only OA stimulation group.</p

HepG2 cells were incubated with 0.2 mmol/L OA only or with different concentrations of mangiferin (12.5, 25, 50, 100 µmol/L) simultaneously for 24 h.

<p>Proteins were isolated from the cell lysates and analyzed by western blot analysis for AMPK (A), CD36 (B), CPT1 (C), ACC (D) and DGAT (E) expressions. The experiments were repeated 3 times. Data are presented as means ± SD (n = 3). ^*<i>P</i><0.05 compared with only OA stimulation group.</p

Effects of mangiferin on the ratio of AMP to ATP and LKB1 protein expression in HepG2 cells.

<p>HepG2 cells were incubated to 0.2 mmol/L oleic acid only or with different concentrations of mangiferin (12.5, 25, 50, 100 µmol/L) simultaneously for 24 h. The ratio of AMP to ATP was detected by HPLC (A). The LKB1 protein expression was carried out by western blot analysis (B). The experiments were repeated 3 times. Data are presented as means ± SD (n = 3). ^*<i>P</i><0.05 compared with only OA stimulation group.</p

Effect of mangiferin on fasting metabolic variables at 6 weeks in hyperlipemic rats.

<p>Data are means ± SD (n = 10),</p>#<p><i>P</i><0.05</p>##<p><i>P</i><0.01 indicate statistically significant differences when compared with control group.</p><p>*<i>P</i><0.05 and</p><p>**<i>P</i><0.01 indicate statistically significant differences when compared with hyperlipidemia group.</p

Effects of Histidine Supplementation on Global Serum and Urine ¹H NMR-based Metabolomics and Serum Amino Acid Profiles in Obese Women from a Randomized Controlled Study

The aim of current study was to investigate the metabolic changes associated with histidine supplementation in serum and urine metabolic signatures and serum amino acid (AA) profiles. Serum and urine ¹H NMR-based metabolomics and serum AA profiles were employed in 32 and 37 obese women with metabolic syndrome (MetS) intervened with placebo or histidine for 12 weeks. Multivariable statistical analysis were conducted to define characteristic metabolites. In serum ¹H NMR metabolic profiles, increases in histidine, glutamine, aspartate, glycine, choline, and trimethylamine-<i>N</i>-oxide (TMAO) were observed; meanwhile, decreases in cholesterol, triglycerides, fatty acids and unsaturated lipids, acetone, and α/β-glucose were exhibited after histidine supplement. In urine ¹H NMR metabolic profiles, citrate, creatinine/creatine, methylguanidine, and betaine + TMAO were higher, while hippurate was lower in histidine supplement group. In serum AA profiles, 10 AAs changed after histidine supplementation, including increased histidine, glycine, alanine, lysine, asparagine, and tyrosine and decreased leucine, isoleucine, ornithine, and citrulline. The study showed a systemic metabolic response in serum and urine metabolomics and AA profiles to histidine supplementation, showing significantly changed metabolism in AAs, lipid, and glucose in obese women with MetS

Sílabo de Derecho Laboral

La asignatura es un curso formativo para el estudiante de contabilidad necesariamente vinculado a la empresa o a personas naturales con negocios donde se desarrollan relaciones laborales y por lo tanto se generan derechos y obligaciones para los empleadores y trabajadores. El curso es de naturaleza teórica – práctica y tiene por objeto dotar al estudiante de los conocimientos que le permitan liquidar los beneficios sociales y demás derechos laborales a fin de cumplir de manera eficiente su actividad profesional

The trend of overall obesity and abdominal obesity in three curves with an increase in age strata.

<p>There was no significant difference in obesity rates between users of calcium supplements and non-users of calcium supplements at each age stratum. Overall obesity, BMI ≥28 kg/m²; abdominal obesity І, WC ≥85 cm for men or WC ≥80 cm for women; abdominal obesity П, WHR ≥0.90 for men or WHR ≥0.85 for women.</p

Mouse PPIN topological properties of TF-targets vs NON-TF-targets.

<p>Mouse PPIN topological properties of TF-targets vs NON-TF-targets.</p