Spectroscopic Measurements of the Far-Ultraviolet Dust Attenuation Curve at z~3

We present the first measurements of the shape of the far-ultraviolet (far-UV; lambda=950-1500 A) dust attenuation curve at high redshift (z~3). Our analysis employs rest-frame UV spectra of 933 galaxies at z~3, 121 of which have very deep spectroscopic observations (>7 hrs) at lambda=850-1300 A, with the Low Resolution Imaging Spectrograph on the Keck Telescope. By using an iterative approach in which we calculate the ratios of composite spectra in different bins of continuum color excess, E(B-V), we derive a dust curve that implies a lower attenuation in the far-UV for a given E(B-V) than those obtained with standard attenuation curves. We demonstrate that the UV composite spectra of z~3 galaxies can be modeled well by assuming our new attenuation curve, a high covering fraction of HI, and absorption from the Lyman-Werner bands of H2 with a small (<20%) covering fraction. The low covering fraction of H2 relative to that of the HI and dust suggests that most of the dust in the ISM of typical galaxies at z~3 is unrelated to the catalysis of H2, and is associated with other phases of the ISM (i.e., the ionized and neutral gas). The far-UV dust curve implies a factor of ~2 lower dust attenuation of Lyman continuum (ionizing) photons relative to those inferred from the most commonly assumed attenuation curves for L* galaxies at z~3. Our results may be utilized to assess the degree to which ionizing photons are attenuated in HII regions or, more generally, in the ionized or low column density (N(HI)<10^17.2 cm^-2) neutral ISM of high-redshift galaxies.Comment: 12 pages, 1 table, 8 figures, accepted to the Astrophysical Journa

X-Ray and Radio Emission from UV-Selected Star Forming Galaxies at Redshifts 1.5<Z<3.0 in the GOODS-North Field

We have examined the stacked radio and X-ray emission from UV-selected galaxies spectroscopically confirmed to lie between redshifts 1.5 < z < 3.0 in the GOODS-North field to determine their average extinction and star formation rates (SFRs). The X-ray and radio data are obtained from the Chandra 2 Msec survey and the Very Large Array, respectively. There is a good agreement between the X-ray, radio, and de-reddened UV estimates of the average SFR for our sample of z~2 galaxies of ~50 solar masses per year, indicating that the locally-calibrated SFR relations appear to be statistically valid from redshifts 1.5 < z < 3.0. We find that UV-estimated SFRs (uncorrected for extinction) underestimate the bolometric SFRs as determined from the 2-10 keV X-ray luminosity by a factor of ~4.5 to 5.0 for galaxies over a large range in redshift from 1.0 < z < 3.5.Comment: 5 pages, 1 figure, Accepted by ApJ Letter

The Connection Between Reddening, Gas Covering Fraction, and the Escape of Ionizing Radiation at High Redshift

We use a large sample of galaxies at z~3 to establish a relationship between reddening, neutral gas covering fraction (fcov(HI)), and the escape of ionizing photons at high redshift. Our sample includes 933 galaxies at z~3, 121 of which have very deep spectroscopic observations (>7 hrs) in the rest-UV (lambda=850-1300 A) with Keck/LRIS. Based on the high covering fraction of outflowing optically-thick HI indicated by the composite spectra of these galaxies, we conclude that photoelectric absorption, rather than dust attenuation, dominates the depletion of ionizing photons. By modeling the composite spectra as the combination of an unattenuated stellar spectrum including nebular continuum emission with one that is absorbed by HI and reddened by a line-of-sight extinction, we derive an empirical relationship between E(B-V) and fcov(HI). Galaxies with redder UV continua have larger covering fractions of HI characterized by higher line-of-sight extinctions. Our results are consistent with the escape of Lya through gas-free lines-of-sight. Covering fractions based on low-ionization interstellar absorption lines systematically underpredict those deduced from the HI lines, suggesting that much of the outflowing gas may be metal-poor. We develop a model which connects the ionizing escape fraction with E(B-V), and which may be used to estimate the escape fraction for an ensemble of high-redshift galaxies. Alternatively, direct measurements of the escape fraction for our data allow us to constrain the intrinsic 900-to-1500 A flux density ratio to be >0.20, a value that favors stellar population models that include weaker stellar winds, a flatter initial mass function, and/or binary evolution. Lastly, we demonstrate how the framework discussed here may be used to assess the pathways by which ionizing radiation escapes from high-redshift galaxies. [Abridged]Comment: 22 pages, 3 tables, 14 figures, accepted to the Astrophysical Journa

Transcriptional and Epigenetic Regulation of Cerebellar Development and Function

Precise control of gene expression is essential for neural development and function. This control is regulated by the interplay of chromatin remodelers and transcription factors (TFs). To better understand these mechanisms involved in gene regulation, we pursue two questions: 1) what are the roles of the chromatin remodeler CHD7 in cerebellar development and 2) what are the roles of the MEF2 TF family in cerebellar function. CHD7 mutations are causative for CHARGE syndrome, a heterogeneous disorder affecting many organ systems, occurring in 1:10,000 newborns. Recent MRI studies have identified cerebellar hypoplasia and foliation defects in a large portion of CHARGE syndrome patients. To identify the how a decrease in CHD7 activity leads to cerebellar defects seen in patients, we conditionally knockout CHD7 in granule cell precursors of mouse cerebellum. Surprisingly, we see a stereotyped folding pattern along the typically smooth mediolateral axis. We then characterize cellular processes and identify a switch in the preferred axis of granule cell precursor division prior to onset of irregular folding. Upon assessing transcriptomic and epigenomic functions of CHD7, we find CHD7 to regulate gene programs implicated in human disorders of brain folding. Additionally, we show that CHD7 reduces accessibility of enhancers with corresponding reduction in enhancer activity and proximal gene expression. The recruitment of chromatin remodelers to specific regions is accomplished by multiple factors including transcription factors. Thus, TFs also play a vital role in proper gene regulation. Furthermore, transcription factors can bind accessible regions and recruit other factors important for transcription to occur. The latter part of this dissertation will address the roles of paralogous transcription factors, MEF2A and MEF2D, and their roles in cerebellar function. MEF2A and MEF2D are highly expressed in cerebellar granule cells, the most abundant neuron in the brain. Using single and double conditional knockout of MEF2 family TFs, we find that MEF2A and MEF2D play functionally redundant roles in cerebellar-dependent motor learning. Although both TFs are highly expressed in granule neurons, transcriptomic analyses show MEF2D is the predominant genomic regulator of gene expression in vivo. Strikingly, genome-wide occupancy analyses reveal upon depletion of MEF2D, MEF2A occupancy robustly increases at a subset of sites normally bound to MEF2D. Importantly, sites experiencing compensatory MEF2A occupancy are concentrated within open chromatin and undergo functional compensation for genomic activation and gene expression. Finally, motor activity induces a switch from non-compensatory to compensatory MEF2-dependent gene regulation. These studies uncover genome-wide functional interdependency between paralogous TFs in the brain. Collectively, our studies on CHD7 and MEF2 provide further understanding of the roles of two critical classes of gene regulators in cerebellar development and function. These studies have set the basis for understanding how TFs and chromatin remodelers control gene expression. Future studies will address how knockout of chromatin remodelers affect TF occupancy, and conversely, how TF depletion prevents chromatin remodeler recruitment

A High-Resolution Hubble Space Telescope Study of Apparent Lyman Continuum Leakers at z∼3z\sim3

We present $U_{336}V_{606}J_{125}H_{160}$ follow-up $HST$ observations of 16 $z\sim3$ candidate LyC emitters in the HS1549+1919 field. With these data, we obtain high spatial-resolution photometric redshifts of all sub-arcsecond components of the LyC candidates in order to eliminate foreground contamination and identify robust candidates for leaking LyC emission. Of the 16 candidates, we find one object with a robust LyC detection that is not due to foreground contamination. This object (MD5) resolves into two components; we refer to the LyC-emitting component as MD5b. MD5b has an observed 1500\AA\ to 900\AA\ flux-density ratio of $(F_{UV}/F_{LyC})_{obs}=4.0\pm2.0$, compatible with predictions from stellar population synthesis models. Assuming minimal IGM absorption, this ratio corresponds to a relative (absolute) escape fraction of $f_{esc,rel}^{MD5b}=75-100$% ($f_{esc,abs}^{MD5b}=14-19$%). The stellar population fit to MD5b indicates an age of $\lesssim50$Myr, which is in the youngest 10% of the $HST$ sample and the youngest third of typical $z\sim3$ Lyman break galaxies, and may be a contributing factor to its LyC detection. We obtain a revised, contamination-free estimate for the comoving specific ionizing emissivity at $z=2.85$, indicating (with large uncertainties) that star-forming galaxies provide roughly the same contribution as QSOs to the ionizing background at this redshift. Our results show that foreground contamination prevents ground-based LyC studies from obtaining a full understanding of LyC emission from $z\sim3$ star-forming galaxies. Future progress in direct LyC searches is contingent upon the elimination of foreground contaminants through high spatial-resolution observations, and upon acquisition of sufficiently deep LyC imaging to probe ionizing radiation in high-redshift galaxies.Comment: 31 pages, 5 tables, 19 figures. Accepted to ApJ. Version with full-resolution figures is available at: http://www.astro.ucla.edu/~aes/Mostardi_HST_LyC.pd

Investigating Hα, UV, and IR Star-formation Rate Diagnostics for a Large Sample of z ~ 2 Galaxies

We use a sample of 262 spectroscopically confirmed star-forming galaxies at redshifts 2.08 ≤ z ≤ 2.51 to compare Hα, ultraviolet (UV), and IR star formation rate (SFR) diagnostics and to investigate the dust properties of the galaxies. At these redshifts, the Hα line shifts to the K_s band. By comparing K_s-band photometry to underlying stellar population model fits to other UV, optical, and near-infrared data, we infer the Hα flux for each galaxy. We obtain the best agreement between Hα- and UV-based SFRs if we assume that the ionized gas and stellar continuum are reddened by the same value and that the Calzetti attenuation curve is applied to both. Aided with MIPS 24 μm data, we find that an attenuation curve steeper than the Calzetti curve is needed to reproduce the observed IR/UV ratios of galaxies younger than 100 Myr. Furthermore, using the bolometric SFR inferred from the UV and mid-IR data (SFR_(IR)+SFR_(UV), we calculated the conversion between the Hα luminosity and SFR to be (7.5 ± 1.3) x 10^(-42) for a Salpeter initial mass function, which is consistent with the Kennicutt conversion. The derived conversion factor is independent of any assumption of the dust correction and is robust to stellar population model uncertainties

Q1549-C25: A Clean Source of Lyman-Continuum Emission at z=3.15z=3.15

We present observations of Q1549-C25, an ~L* star-forming galaxy at z=3.15 for which Lyman-continuum (LyC) radiation is significantly detected in deep Keck/LRIS spectroscopy. We find no evidence for contamination from a lower-redshift interloper close to the line of sight in the high signal-to-noise spectrum of Q1549-C25. Furthermore, the morphology of Q1549-C25 in V_606, J_125, and H_160 Hubble Space Telescope (HST) imaging reveals that the object consists of a single, isolated component within 1". In combination, these data indicate Q1549-C25 as a clean spectroscopic detection of LyC radiation, only the second such object discovered to date at z~3. We model the spectral energy distribution (SED) of Q1549-C25, finding evidence for negligible dust extinction, an age (assuming continuous star formation) of ~1 Gyr, and a stellar mass of M_*=7.9x10^9 M_sun. Although it is not possible to derive strong constraints on the absolute escape fraction of LyC emission, f_esc(LyC), from a single object, we use simulations of intergalactic and circumgalactic absorption to infer f_esc(LyC)>=0.51 at 95% confidence. The combination of deep Keck/LRIS spectroscopy and HST imaging is required to assemble a larger sample of objects like Q1549-C25, and obtain robust constraints on the average f_esc(LyC) at z~3 and beyond.Comment: 6 pages, 5 figures, accepted to ApJ Letter

A High Fraction of Ly-alpha-Emitters Among Galaxies with Extreme Emission Line Ratios at z ~ 2

Star-forming galaxies form a sequence in the [OIII]/H-beta vs. [NII]/H-alpha diagnostic diagram, with low metallicity, highly ionized galaxies falling in the upper left corner. Drawing from a large sample of UV-selected star-forming galaxies at z~2 with rest-frame optical nebular emission line measurements from Keck-MOSFIRE, we select the extreme ~5% of the galaxies lying in this upper left corner, requiring log([NII]/H-alpha) = 0.75. These cuts identify galaxies with 12 + log(O/H) <~ 8.0, when oxygen abundances are measured via the O3N2 diagnostic. We study the Ly-alpha properties of the resulting sample of 14 galaxies. The mean (median) rest-frame Ly-alpha equivalent width is 39 (36) A, and 11 of the 14 objects (79%) are Ly-alpha-emitters (LAEs) with W_Lya > 20 A. We compare the equivalent width distribution of a sample of 522 UV-selected galaxies at 2.0<z<2.6 identified without regard to their optical line ratios; this sample has mean (median) Ly-alpha equivalent width -1 (-4) A, and only 9% of these galaxies qualify as LAEs. The extreme galaxies typically have lower attenuation at Ly-alpha than those in the comparison sample, and have ~50% lower median oxygen abundances. Both factors are likely to facilitate the escape of Ly-alpha: in less dusty galaxies Ly-alpha photons are less likely to be absorbed during multiple scatterings, while the harder ionizing spectrum and higher ionization parameter associated with strong, low metallicity star formation may reduce the covering fraction or column density of neutral hydrogen, further easing Ly-alpha escape. The use of nebular emission line ratios may prove useful in the identification of galaxies with low opacity to Ly-alpha photons across a range of redshifts.Comment: 12 pages, 5 figures, 2 tables. Accepted for publication in Ap