Similarity between existing and new <i>T. scripta</i> sequences.

<p>Existing <i>T. scripta</i> sequences in Genbank were used as queries in a BLASTN search of our assembled sequences. The BLAST HSP sizes represent the sizes of the sequence matches between existing sequences and new <i>T. scripta</i> transcriptome assembly sequences.</p

TCA cycle enzymes present in the <i>T. scripta</i> developmental transcriptome.

<p>Predicted proteins in the transcriptome were mapped to the TCA KEGG metabolic pathway using Blast2Go.</p

Top protein hits by species.

<p>The number of top BLAST hits and the number of sequences in the NCBI protein database per species were used to calculate the enrichment in top hits per species relative to the number of sequences in the database. The top half of the table lists the top nine species, the bottom half compares the combined hits for all birds with the hits to the Carolina Anole Lizard.</p

RT-PCR of developmentally important genes from a stage 17 <i>T. scripta</i> cDNA pool.

<p>RT-PCR of developmentally important genes from a stage 17 <i>T. scripta</i> cDNA pool.</p

<i>BMP5</i> expression in a stage 15 <i>T. scripta</i> embryo.

<p><i>BMP5</i> expression is associated with the developing vertebrae in the cervical region and the newly formed somites in the tailbud. In addition, BMP5 is expressed in the anterior and posterior margins of the autopod, and in the apical ectodermal ridge of the developing limb buds (A and B antisense, C sense).</p

Identification of <i>T. scripta BMP2</i>-<i>7</i> genes.

<p>The <i>T. scripta</i> transcriptome was queried with BMP protein sequences from other organisms. Sequences were aligned and excessively gapped positions were removed (final size of dataset = 285aa/species). Their ML relationships were inferred using MetaPIGA. Labels on nodes indicate posterior probabilities. Scale bar units are the number of amino acid substitutions per site. Accession numbers are to the right of each sequence name.</p