Effect of gibberellin on seeded Vitis vinifera with special reference to induction of seedlessness

Application of GA (25-50 ppm) to Ana:b-e-Shahi at prebloom stage decreased the bunch weight and berry number but weight of the berries was increased and up to 29.6% seedless berries were induced. GA sprays (25-50 ppm) at 2-3 days after full lbloom increased the bunch and berry weights but few seedless berries were induced.GA application (50-150 ppm) to Bhokri grape at 10-11 days before full bloom formed more than 90% seedless berries but bunch weights and berry number were reduced. WitJh an additional second spray at 7- 8 days after full bloom, berry size was impmved but bunch weights were still reduced. At 4-5 days before full bloom application of GA, up to 41.4 % seedless berries were induced but bunch and berry weights were a,gain reduced. With another spray at postbloom stage bunch and berry weights were brought to the level of control.   In Gros Colman (Pusa) grape, GA application (50-100 ppm) at 5-6 days before full bloom and 2-3 days after full bloom also formed seedless berries but the bunch and berry weights were reduced. IFruit set was also improvP.d at boVh stages of spray. Prebloom GA application (50-150 ppm) to Alamwick grape, produced seedless berries up to 38.29% but ,bunch and berry weights were appreciably decreased. No effect on berry number was noticed.Application of GA (25-75 ppm) at prebloom + posbbloom and postbloom alone decreased the bunch and berry weights and berry number markedly of Bharat Early and Black Hamburg varieties. Toxic effects like shot berry formation and cracking of rachis were noticed. No seedless berries were observed. however, more berries with 1 or 2 seeds were formed at the expense of berries with 3 or 4 seeds. Even GA application at Jow concentrations to these varieties (5-25 ppm) reduced bunch weights and berry number.The differential response of these seeded varieties of Vitis vinifera is attributed to different amounts of growth factors and inhibitors present in them

Differential response of some seeded grape cultivars of Vitis vinifera to gibberellin application

Several seeded cultivar,s of Vitis vinifera varying in seed content and berry size were tested to see their response to GA application. Oultivars with high seed index number and low seed content like Bhokri, Anab-e-Shahi, Gros Colman (Pusa) and Alamwick did not show any toxic effects due to GA application but responded by producing seedless berries and increasing berry weight with prebloom and postbloom application respectively. Alamwick alone, with high seed index number did not respond to GA by increasing the berry weight but it responded by forming seedless berries. Oultivars like Bharat Early, Black Hamburg and Black Muscat with high seed content per berry and low seed index number did not respond to GA by producing seedless berries and increasing berry weight. This differential response of cultivars to exogenous GA is discussed on the basis of physiological differences in the two groups of cultivars

Effects of certain growth substances and boric acid on germination, tube growth and storage of grape pollen (Vitis ssp.)

Investigations on the ,effects of growth substances (GA, IAA and IBA) and boric acid on grape pollen have shown that maximum germination and tube growth was obtained rwith boric acid at lower concentrations (5, 10 and 20 ppm) as compared to GA, IAA, IBA and control. Although GA showed lesser pollen germination, pollen tube growth was enhanced at 5, 10 and 20 ppm in the grape cultivars under study. Pollen grains of Pusa Seedless were stored for 12 months and Pearl of Csaba for four months at different combinations of temperature and ihumidity. The pollen viability was highest when sfored at -12° to -10° C with 25 per cent R.H. which was followed by 4° to 8° C ,and 25 per cent R.H. The pollen germination increased throughout the storage period by boric acid at 5, 10 and 20 ppm

Induction of pollen sterility in grapes (Vitis vinifera)

The morphology of the grape flower renders hand emasculation very tedious and hence hinders large scale hybridisation work. Chemical induction of male sterility which has been successfully achieved in some crops may likewise aid in grape breeding, if the method proves practicable. This paper deals with an experiment designed to achieve this object.Three varieties of grapes, Bhokri, Hussaini and Rosem-T-Lahore (all belonging to Vitis vinifera) were tested with three different chemicals, viz. maleic hydrazide, tri-iodobenzoic acid and FW-450, using various concentrations. In the first two varieties the dosages of maleic hydrazide 500 ppm, tri-iodobenzoic acid 400 ppm and FW-450 0.30% was found to induce complete pollen sterility. However, the third variety needed higher concentrations. In all cases two applications of the chemicals were necessary to induce comp1ete pollen sterility. In addition to inducing pollen sterility, FW-450 prevented anthesis and anther dehiscence. Considering the fruit set and seed set obtained in the male sterile flowers (induced by various chemicals) rby using good pollen, maleic hydrazide appears to be most promising. The chemical treatments did not influence seed germination. The most important factors with regard to the use of this method were  (1) the variety used, (2) the chemical applied, (3) the number of applications of the chemical and(4) the stage at which the chemical treatments are made. The implications of these results have been discussed

Inequalities and outcomes : end stage kidney disease in ethnic minorities

The international evidence about outcomes of End Stage Kidney Disease (ESKD) for ethnic minorities was reviewed to identify gaps and make recommendations for researchers and policy makers. Nine databases were searched systematically with 112 studies from 14 different countries included and analysed to produce a thematic map of the literature. Reviews (n = 26) highlighted different mortality rates and specific causes between ethnic groups and by stage of kidney disease associated with individual, genetic, social and environmental factors. Primary studies focussing on uptake of treatment modalities (n = 19) found ethnic differences in access. Research evaluating intermediate outcomes and quality of care in different treatment phases (n = 35) e.g. dialysis adequacy, transplant evaluation and immunosuppression showed ethnic minorities were disadvantaged. This is despite a survival paradox for some ethnic minorities on dialysis seen in studies of longer term outcomes (n = 29) e.g. in survival time post-transplant and mortality. There were few studies which focussed on end of life care (n = 3) and ethnicity. Gaps identified were: limited evidence from all stages of the ESKD pathway, particularly end of life care; a lack of system oriented studies with a reliance on national routine datasets which are limited in scope; a dearth of qualitative studies; and a lack studies from many countries with limited cross country comparison and learning. Differences between ethnic groups occur at various points and in a variety of outcomes throughout the kidney care system. The combination of individual factors and system related variables affect ethnic groups differently indicating a need for culturally intelligent policy informed by research to prevent disadvantage

Development of a microprocessor-based biochemical sampler

Modern medicine requires patients to be treated on the basis of precise data, which are often obtained from electronic equipment. An inexpensive and portable microprocessor-based sampler developed by the authors is described. It is comprised of the following units: sample plate assembly, probe-drive linkage system, wash fluid receptacle, timing system and 8085A microprocessor

A Systematic Scoping Review of the Interventions Available During Care Crises for People with Learning or Intellectual Disabilities (LD) who are Cared for by an Ageing Carer within the United Kingdom.

The majority of individuals with learning disabilities within the United Kingdom (UK) are cared for by a family member, usually a parent. However, individuals with learning disabilities in the UK are now living longer than ever before [1] which means that the number of ageing family members providing care is also increasing. Furthermore, an increasing number of individuals with LD are now also outliving their parents [2]. Despite this, many adults with LD and their ageing carers do not make plans for their future [2], including periods of care crisis such as the serious illness or death of their ageing primary care provider