5 research outputs found
Penicillin acylase catalyzed synthesis of penicillin-G from substrates anchored in cyclodextrins
6-12Penicillin acylase (EC 3.5.1.11 ) catalyses the
condensation of phenylacetic acid (PAA) and 6-aminopenicillanic acid (6-A A) to
form benzylpenicillin (BP). Both PAA and 6-APA were found to form host-guest
complexes with β-methylcyclodextrin (βm-CD) and γ-cyclodextrin (γ -CD)
respectively. The rate of the reaction catalyzed by the enzyme remained
unaffected if one of the substrates used was in the cyclodextrin complexed form.
However, in this case, the reaction lasted longer and yielded about 20 per cent
more products compared to the condensation reaction involving only uncomplexed
substrates. There was a distinct increase in the rate of formation of the
antibiotic, if both substrates used are in CD-complexed form .</span