Cellulase enzyme production of post-harvest fungi under the influence of carbon and nitrogen sources

Present research work embodied the effect of nutritional sources like carbon and nitrogen on cellulase enzyme production of post-harvest fungi. It was found that, among carbon sources fructose and sucrose stimulated cellulase activity, while lactose, CMC and starch inhibited the cellulase activity of test fungi. Nitrate source like sodium nitrate, sodium nitrite and calcium nitrate stimulated the cellulase activity while, ammonium sources in the form of nitrate, phosphate and sulphate were proved inhibitory for cellulase production of all post-harvest fungi

Impact of carbon and nitrogen sources on pectinase production of post-harvest fungi

In the present investigation, emphasis has been given on to study the pectinase enzyme production of post-harvest fungi isolated from mango and papaya fruits, under the influence of carbon and nitrogen sources. It was found that, among carbon sources fructose and sucrose induced pectinase activity, while lactose, CMC and starch inhibited the pectinase activity of test fungi. Nitrate source like sodium nitrate, sodium nitrite and calcium nitrate were found to be stimulated the pectinase activity while, ammonium sources in the form of nitrate, phosphate and sulphate were proved inhibitory for pectinase production of all post-harvest fungi

Kinetics and mechanism of oxidation of aliphatic alcohols by oxone catalyzed by Keggin type 12-tungstocobaltate(II)

1626-1630The oxidation of various aliphatic alcohols by ox one catalyzed by Kegin type [CoIIW12O40]6- has been studied in a buffer medium of pH = 4. The reaction proceeds by the oxidation of [CoIIW12O40]6- to [CoIIIW12O40]5- by oxone which then oxidizes the alcohol in a rate  determining step generating alcohol free radical. The reaction is accelerated by increase in the pH of the solution due to the protonation equilibria of the oxidant. The reaction between oxone and [CoIIW12O40]6- has also studied and is found to proceed in two one-electron steps, involving formation HSO52-  free radical in a slow first step followed by its reaction with reductant in a fast step. The reaction rate is inhibited by the [H+] due to protonation equili bria of HSO5- which is the active species. Decreasing the relative permittivity of the medium increases the rate of the reaction which is attributed to the formation of an outer-sphere complex between the reactants. The activation parameters determined for both the reactions support the proposed mechanisms