The development of radiogallium-acetylacetonate bis(thiosemicarbazone) complex for tumour imaging

BACKGROUND: Various radiometal complexes have been developed for tumour imaging, especially Ga-68 tracer. In this work, the development of a radiogallium bis(thiosemicarbazone) complex has been reported. MATERIAL AND METHODS: [67Ga]acetylacetonate bis(thio-semicarbazone) complex ([67Ga]AATS) was prepared starting with [67Ga]Gallium acetate and freshly prepared acetylacetonate bis(thiosemicarbazone) (AATS) for 30 min at 90ºC. The partition co-efficient and stability of the tracer was determined in final solution (25ºC) and the presence of human serum (37ºC) for up to 24 hours. The biodistribution of the labelled compound in wild-type and fibrosarcoma-bearing rodents were determined for up to 72 hours. RESULTS: The radiolabelled Ga complex was prepared to a high radiochemical purity (> 97%, HPLC) followed by initial biodistribution data with the significant tumour accumulation of the tracer at two hours, which is far higher than free Ga-67 cation, while the compound wash-out is significantly faster. CONCLUSION: The above-mentioned pharmacokinetic properties suggest an interesting radiogallium complex prepared by the PET Ga radioisotope, 68Ga, in accordance with the physical half life, for use in fibrosarcoma tumours and possibly in other malignancies

Evaluation of [ 67 Ga]Citrate in The Detection of Various Microorganism Infections in Animal Models

ABSTRACT Introduction: Gallium-67 citrate has been known as a good infection agent in nuclear medicine for decades. In this work the value of 67 Ga-citrate has been investigated in infected animal models using SPECT imaging at optimized/standardized conditions. Methods: The bacterial (Staphylococcus aureus; S.a. and Escherichia coli; E.c.) and fungal (Candidae albicans; C.a.) species from standard sources were cultured according to the standard procedures and wild-type NMRI rats were inoculated by the injection of 5x10 7 microorganisms (MO) into their thighs and animals incubated for infection site formation for 2 and 3 days followed by iv injection of freshly prepare