2 research outputs found
<i>J</i>‑Resolved <sup>1</sup>H NMR 1D-Projections for Large-Scale Metabolic Phenotyping Studies: Application to Blood Plasma Analysis
No full text<sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy-based
metabolic phenotyping is now widely used for large-scale epidemiological
applications. To minimize signal overlap present in 1D <sup>1</sup>H NMR spectra, we have investigated the use of 2D <i>J</i>-resolved (JRES) <sup>1</sup>H NMR spectroscopy for large-scale phenotyping
studies. In particular, we have evaluated the use of the 1D projections
of the 2D JRES spectra (pJRES), which provide single peaks for each
of the <i>J</i>-coupled multiplets, using 705 human plasma
samples from the FGENTCARD cohort. On the basis of the assessment
of several objective analytical criteria (spectral dispersion, attenuation
of macromolecular signals, cross-spectral correlation with GC-MS metabolites,
analytical reproducibility and biomarker discovery potential), we
concluded that the pJRES approach exhibits suitable properties for
implementation in large-scale molecular epidemiology workflows
