Development of formative assessment tool for a primary, technology classroom

Teachers and students' interactions in the classroom include a large number of questions, some of which are a key part of formative assessment. Questions can lead to an extended dialogue between the teacher and the student, potentially facilitating a better understanding of the students' conceptions and providing teachers with information to guide student learning. Technology Observation and Conversation Framework (TOCF) was identified as a framework of questions specially designed for a technology classroom. In this qualitative, design-based research, the TOCF was modified for alignment with the New Zealand curriculum and provided to two primary teachers teaching ages 9-10. The version of TOCF reported in this article was developed through an iterative process in an authentic environment. The teachers were interviewed periodically, and modifications were made to the format of the framework. The findings in this paper focus on the outputs of the iterative process and the feedback given by the teachers on the TOCF. While teachers in the study found the questions crucial to deepen student thinking in technology, they faced some constraints in using the TOCF in the classroom. The findings suggest that any introduction of a new resource should proceed slowly in the classroom and time needs to be given for increasing familiarity with the new resource. It is also possible that inexperienced teachers could find adoption of questioning practice quite tricky and would need to be supported extensively to change their practice

Acute- and late-phase matrix metalloproteinase (MMP)-9 activity is comparable in female and male rats after peripheral nerve injury

Abstract Background In the peripheral nerve, pro-inflammatory matrix metalloproteinase (MMP)-9 performs essential functions in the acute response to injury. Whether MMP-9 activity contributes to late-phase injury or whether MMP-9 expression or activity after nerve injury is sexually dimorphic remains unknown. Methods Patterns of MMP-9 expression, activity and excretion were assessed in a model of painful peripheral neuropathy, sciatic nerve chronic constriction injury (CCI), in female and male rats. Real-time Taqman RT-PCR for MMP-9 and its endogenous inhibitor, tissue inhibitor of metalloproteinase-1 (TIMP-1) of nerve samples over a 2-month time course of CCI was followed by gelatin zymography of crude nerve extracts and purified MMP-9 from the extracts using gelatin Sepharose-beads. MMP excretion was determined using protease activity assay of urine in female and male rats with CCI. Results The initial upsurge in nerve MMP-9 expression at day 1 post-CCI was superseded more than 100-fold at day 28 post-CCI. The high level of MMP-9 expression in late-phase nerve injury was accompanied by the reduction in TIMP-1 level. The absence of MMP-9 in the normal nerve and the presence of multiple MMP-9 species (the proenzyme, mature enzyme, homodimers, and heterodimers) was observed at day 1 and day 28 post-CCI. The MMP-9 proenzyme and mature enzyme species dominated in the early- and late-phase nerve injury, consistent with the high and low level of TIMP-1 expression, respectively. The elevated nerve MMP-9 levels corresponded to the elevated urinary MMP excretion post-CCI. All of these findings were comparable in female and male rodents. Conclusion The present study offers the first evidence for the excessive, uninhibited proteolytic MMP-9 activity during late-phase painful peripheral neuropathy and suggests that the pattern of MMP-9 expression, activity, and excretion after peripheral nerve injury is universal in both sexes

Graphene oxide as a chemically tunable platform for optical applications

10.1038/nchem.907Nature Chemistry2121015-102