Kaplan-Meier overall survival curves of NPC patients with expression of p-Mnk1 and p-eIF4E protein and different clinicopathological characteristics.

<p>Kaplan-Meier analysis to plot the survival curve of all 272 NPC patients with expression of p-Mnk1 and p-eIF4E and different clinicopathological characteristics and statistical significance was assessed using the log-rank test. Fig. 3A. Kaplan-Meier curves showed worse overall survival for p-Mnk1–positive patients compared with p-Mnk1–negative patients (P<0.001, two sided). Fig. 3B. Kaplan-Meier curves showed worse survival for p-eIF4E–positive patients compared with p-eIF4E–negative patients (P = 0.004, two sided). Fig. 3C. NPC patients with clinical stage III and IV were significantly related to poor prognosis compared to those patients with clinical stage I and II (P = 0.003, two sided). Fig. 3D. NPC patients with lymph node metastasis were significantly related to poor prognosis compared to those patients without lymph node metastasis (P<0.001, two sided). Fig. 3E NPC patients with combination chemotherapy and radiotherapy were significantly related to good prognosis compared to patients with chemotherapy and radiotherapy alone (P = 0.006, two sided). Fig. 3F. Histological types of NPC patients were no significantly related to their prognosis (P>0.05, two sided).</p

Expression of EGFR protein in lung SCC cells, lung ADC cells and the control of non-cancerous lung tissue were detected by IHC using specific antibody as described in the section of materials and methods.

<p>Strong positive staining of EGFR protein was found in cell membranes and cytoplasm of lung SCC and lung ADC cells (Fig 2A and 2B, 20×, IHC, DAB staining). Negative staining of EGFR was showed in non-cancerous lung tissue (Fig 2C, 20×, IHC, DAB staining). Negative control showed no EGFR staining in the lung SCC cells (Fig 2D, 20×, IHC, DAB staining).</p

Association between expression of p-Mnk1 and p-eIF4E protein and clinicopathological features of NPC (n = 272).

<p>Abbreviations: NS, non significant; DNKC: Differentiated non-keratinized carcinoma, UDC: Undifferentiated carcinoma; LN, lymph node; LN, LNM, lymph node metastasis.</p

Expression of Flot-2 and EGFR proteins in lung SCC and lung ADC compared to the control of non-cancerous lung tissues.

<p>Results showed that there were significant differences between the groups which were statistically evaluated by chi-square test.</p

The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.

<p>The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.</p

Kaplan-Meier cures for overall survival of lung SCC and ADC patients with expression of Flot-2 and EGFR.

<p>Kaplan-Meier analysis was used to plot the overall survival curves of 159 cases of lung SCC and 193 cases of lung ADC patients with differential expression of Flot-2, EGFR, and combined expression of either of these two proteins, which statistical significance was assessed by log-rank test. (A) Lung SCC patients with positive expression of Flot-2 protein showed worse overall survival rates compared to patients with negative Flot-2 (P = 0.043, two sided). (B) Positive expression of EGFR had no significantly correlation with overall survival rates of lung SCC patients (P> 0.05, two sided). (C) Kaplan-Meier curves showed lung SCC patients with positive expression with either of Flot-2 and EGFR proteins had worse overall survival rates than these with all negative staining of two proteins above (P = 0.02, two sided). (D) Lung ADC patients with positive expression of Flot-2 had worse overall survival rates than that with negative one (P = 0.007, two sided, respectively). (E) Lung ADC patients with positive expression of EGFR had worse overall survival rates than that with negative one (P = 0.033, two sided, respectively). (F) Lung ADC patients with positive expression with either of Flot-2 and EGFR proteins showed worse overall survival rates compared with all negative staining of two proteins above (P = 0.005, two sided).</p

Expression of p-Mnk1 and p-eIF4E protein in NPC and non-cancerous nasopharyngeal epithelial tissues was detected by immunohistochemistry.

<p>The expression of p-Mnk1 and p-eIF4E protein was detected by immunohistochemistry using specific antibodies as described in the section of materials and methods. Strong positive expression of p-Mnk1 was found in nuclei of NPC (Fig. 1A, 20×, IHC, AEC staining). Low and weak nuclear positive expression of p-Mnk1 was showed in the non-cancerous nasopharyngeal epithelial cells (Fig. 1B, 20×, IHC, AEC staining). Strong positive expression of p-eIF4E in the cytoplasm of NPC (Fig. 1C, 20×, IHC, AEC staining). Weak positive expression of p-eIF4E in the cytoplasm of the non-cancerous nasopharyngeal epithelial cells (Fig. 1D, 20×, IHC, AEC staining).</p

352 cases of non small cell lung cancer (NSCLC) patient’s characteristics.

<p>352 cases of non small cell lung cancer (NSCLC) patient’s characteristics.</p

Expression of Flot-2 protein in lung SCC cells, lung ADC cells and control of non-cancerous lung tissues were detected by IHC using specific antibody as described in the section of materials and methods.

<p>Strong positive staining of Flot-2 protein was found on cell membranes of lung SCC and lung ADC cells (Fig 1A and 1B, 20×, IHC, DAB staining). Negative staining of Flot-2 was showed in non-cancerous lung tissue (Fig 1C, 20×, IHC, DAB staining). Negative control showed no Flot-2 staining in lung ADC cells (Fig 1D, 20×, IHC, DAB staining).</p

Additional file 1: of Increased expression of Cks1 protein is associated with lymph node metastasis and poor prognosis in nasopharyngeal carcinoma

The original data of IHC scoring. The expression of CKS1 and p27KIP1 had been tested in 168 cases of paraffin-embedded tissue from the primary NPC patients and 49 cases of non-cancerous nasopharyngeal control specimen from independent patients by IHC. The percentage of positive cells was divided into five grades (percentage scores):<10% (0), 11–25% (1), 26–50% (2), 51–75%(3), and >75% (4). The intensity of staining was divided into four grades (intensity scores): no staining (0), light brown (1), brown (2),and dark brown (3). Staining positivity was determined by the formula: overall scores = percentage score × intensity score. The total score ranged from 0 to 12, with negative staining (0–1) and positive expression (2–12). At last, 0 presented negative staining and 1 presented positive staining in the data file. (XLS 42 kb