Synthesis of Demissidine by a Ring Fragmentation 1,3-Dipolar Cycloaddition Approach

A synthesis of the steroidal alkaloid demissidine from epiandrosterone is reported. A ring fragmentation reaction that efficiently ruptured the D-ring of a diazo ester derivative of epiandrosterone to provide an aldehyde tethered ynoate product was key to this sequence. Incorporation of the indolizidine framework was achieved by an azomethine ylide 1,3-dipolar cycloaddition

Additional file 8: Table S8. of Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing

KEGG pathways of significantly down-regulated genes in the drought-sensitive tomato M82. (XLSX 18 kb

Additional file 9: Table S9. of Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing

KEGG pathways of significantly down-regulated genes in the drought-tolerant tomato IL9–1. (XLSX 14 kb

Additional file 17: Table S17. of Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing

Expression level of the target genes for the differentially-expressed novel miRNAs identified. (XLSX 45 kb

Additional file 13: Table S13. of Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing

Expression level of the target genes for the differentially-expressed conserved miRNAs identified. (XLSX 29 kb

Rhodium-Catalyzed NH-Indole-Directed C–H Carbonylation with Carbon Monoxide: Synthesis of 6<i>H</i>‑Isoindolo[2,1‑<i>a</i>]indol-6-ones

An efficient synthesis of 6<i>H</i>-isoindolo­[2,1-<i>a</i>]­indol-6-ones through rhodium-catalyzed NH-indole-directed C–H carbonylation of 2-arylindoles with carbon monoxide has been developed. Preliminary mechanistic studies revealed that this reaction proceeds via N–H bond cleavage and subsequent C–H bond cleavage. Reaction monitoring via ESI-MS was used to support the formation of five-membered rhodacycle species in the catalytic cycle