Additional file 2: Figure S2. of Long branch-chains of amylopectin with B-type crystallinity in rice seed with inhibition of starch branching enzyme I and IIb resist in situ degradation and inhibit plant growth during seedling development

Seed and starch weights and their relationships with seedling weight on the weight basis of pre-germinated seeds during seedling growth. (A), dry weight of seed without embryo; (B, C), the relationships between the decreased seed weight and the root (a), shoot (b), and seedling (root + shoot) weight (c) in TQ (B) and TRS (c); (d), dry weight of starch in endosperm; (e, f), the relationships between the decreased starch weight and the root (a), shoot (b), and seedling (soot + shoot) weight (c) in TQ (E) and TRS (F). For (a and d), values are means ± SD from three replicates, and asterisks (*) highlight significant differences between TQ and TRS by Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001). For (b, c, e, and f), values are the means of three replicates, and R indicates the regression coefficient. (TIFF 8768 kb

Additional file 4: Figure S1. of Development of high-lysine rice via endosperm-specific expression of a foreign LYSINE RICH PROTEIN gene

Amino acid content in T14 generation of PA110 and PA64S. (JPG 1752 kb

Nuphar japonicum DC.

原著和名: カハホネ科名: スイレン科 = Nymphaeaceae採集地: 茨城県 牛久沼 (茨城県 牛久沼)採集日:採集者: 櫻井 宏整理番号: JH045478国立科学博物館整理番号: TNS-VS-99547

Characterization of Agronomy, Grain Physicochemical Quality, and Nutritional Property of High-Lysine 35R Transgenic Rice with Simultaneous Modification of Lysine Biosynthesis and Catabolism

Lysine is the first limiting essential amino acid in rice. We previously constructed a series of transgenic rice lines to enhance lysine biosynthesis (35S), down-regulate its catabolism (Ri), or simultaneously achieve both metabolic effects (35R). In this study, nine transgenic lines, three from each group, were selected for both field and animal feeding trials. The results showed that the transgene(s) caused no obvious effects on field performance and main agronomic traits. Mature seeds of transgenic line 35R-17 contained 48–60-fold more free lysine than in wild type and had slightly lower apparent amylose content and softer gel consistency. Moreover, a 35-day feeding experiment showed that the body weight gain, food efficiency, and protein efficiency ratio of rats fed the 35R-17 transgenic rice diet were improved when compared with those fed wild-type rice diet. These data will be useful for further evaluation and potential commercialization of 35R high-lysine transgenic rice

Inhibitory effect of commercial rhIGFBP-3 on human MCF-7 breast cancer cells and HT-29 colon cancer cells.

<p>Different concentrations of commercial rhIGFBP-3 ranged from 9.375 ng/ml to 300 ng/ml were used to treat MCF-7 and HT-29 cells. Data are shown as means ± SD.</p

Additional file 2: Figure S1. of Ability of Rf5 and Rf6 to Restore Fertility of Chinsurah Boro II-type Cytoplasmic Male Sterile Oryza Sativa (ssp. Japonica) Lines

Temperatures during August in 2011 (a) and 2013 (b). (DOCX 132 kb

Inhibitory effect of different concentration of rice-produced rhIGFBP-3 on HT-29 human colon cancer cells.

<p>Different concentration of seed proteins (ranged from 1.042 to 15.625 mg) from WT and transgenic SBK-66 and SB-57 lines were used to treat HT-29 cells. Data are shown as means ± SD. *<i>p</i> < 0.05 and ** <i>p</i> < 0.01 denote statistically significant and very significant differences, respectively, between transgenic lines and WT.</p

Transgenic integration of modified hIGFBP-3 in rice.

<p>Genomic DNA extracted from rice leaves of independent transformants was digested with BamHI and separated on 0.8% agarose gel, blotted on nylon membrane and hybridized with DIG-labeled hIGFBP-3 probe. Lanes 1-3: three independent pSB130/Gt1/hIGFBP-3 transformants (B1 to B3); lane 4: wild type (WT) rice plant; lanes 5-6: two independent pSB130/Gt1/SP/hIGFBP-3 transformants (SB1 to SB2); lanes 7-9: three independent pSB130/Gt1/SP/hIGFBP-3::KDEL transformants (SBK1 to SBK3).</p

Inhibitory effect of rice-produced rhIGFBP-3 on MCF-7 human breast cancer cells.

<p>(A) Equal amount (3.125 mg seed) of WT, SB and SBK total seed protein extracts were added to the MCF-7 cells separately. Data are shown as means ± SD. *<i>p</i> < 0.05 and ** <i>p</i> < 0.01 denote statistically significant and very significant differences, respectively, between transgenic lines and WT.</p

Glycoprotein staining of transgenic lines and WT line in rice seeds.

<p>Total protein was extracted from mature dehulled seeds of SB-57 and SBK-66 transgenic lines and WT. RNase B was used as the glycoprotein control. To evaluate endoglycosidase digestion efficiency, RNase B was digested by PNGase F and Endo H. Lane 1: marker; lane 2: WT; lane 3: SBK-66; lane 4: SK-57; lane 5: RNase B control without digestion; lane 6: RNase B digested by PNGase F; and lane 7: RNase B digested by Endo H. Band 1: PNGase F; band 2: RNase B; band 3: RNase B with Endo H digestion, and band 4: RNase B with PNGase F digestion.</p