660 research outputs found

    The Impact of Social Media on Brand Equity : A Quantitative Study

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    The development of social media poses tremendous impact on brand equity. Yet, systematic studies regarding how social media impacts brand equity is limited. As a result, managers and executives cannot make full use of the branding opportunities or mitigate risks. This paper is devoted to closing this gap. As the major reason why many executives are reluctant or unable to develop effective social media strategies is due to a lack of understanding about it, thus this paper illustrates in detail what social media is. In order to find out how social media impacts brand equity, a quantitative research is conducted to study brand equity factors based on Aaker’s brand equity model, find out each factor’s individual contribution to brand equity and social media’s impact on those individual factors. A clear understanding of how social media impacts brand equity is formed at the end of the research. Based on these insightful findings, recommendations are provided for organizations with a vision to enhance brand equity

    De Novo Transcriptome of the Hemimetabolous German Cockroach (\u3ci\u3eBlattella germanica\u3c/i\u3e)

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    Background: The German cockroach, Blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. This insect has long served as a model system for studies of insect biology, physiology and ecology. However, the lack of genome or transcriptome information heavily hinder our further understanding about the German cockroach in every aspect at a molecular level and on a genome-wide scale. To explore the transcriptome and identify unique sequences of interest, we subjected the B. germanica transcriptome to massively parallel pyrosequencing and generated the first reference transcriptome for B. germanica. Methodology/Principal Findings: A total of 1,365,609 raw reads with an average length of 529 bp were generated via pyrosequencing the mixed cDNA library from different life stages of German cockroach including maturing oothecae, nymphs, adult females and males. The raw reads were de novo assembled to 48,800 contigs and 3,961 singletons with highquality unique sequences. These sequences were annotated and classified functionally in terms of BLAST, GO and KEGG, and the genes putatively coding detoxification enzyme systems, insecticide targets, key components in systematic RNA interference, immunity and chemoreception pathways were identified. A total of 3,601 SSRs (Simple Sequence Repeats) loci were also predicted. Conclusions/Significance: The whole transcriptome pyrosequencing data from this study provides a usable genetic resource for future identification of potential functional genes involved in various biological processes

    De Novo Transcriptome of the Hemimetabolous German Cockroach (\u3ci\u3eBlattella germanica\u3c/i\u3e)

    Get PDF
    Background: The German cockroach, Blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. This insect has long served as a model system for studies of insect biology, physiology and ecology. However, the lack of genome or transcriptome information heavily hinder our further understanding about the German cockroach in every aspect at a molecular level and on a genome-wide scale. To explore the transcriptome and identify unique sequences of interest, we subjected the B. germanica transcriptome to massively parallel pyrosequencing and generated the first reference transcriptome for B. germanica. Methodology/Principal Findings: A total of 1,365,609 raw reads with an average length of 529 bp were generated via pyrosequencing the mixed cDNA library from different life stages of German cockroach including maturing oothecae, nymphs, adult females and males. The raw reads were de novo assembled to 48,800 contigs and 3,961 singletons with highquality unique sequences. These sequences were annotated and classified functionally in terms of BLAST, GO and KEGG, and the genes putatively coding detoxification enzyme systems, insecticide targets, key components in systematic RNA interference, immunity and chemoreception pathways were identified. A total of 3,601 SSRs (Simple Sequence Repeats) loci were also predicted. Conclusions/Significance: The whole transcriptome pyrosequencing data from this study provides a usable genetic resource for future identification of potential functional genes involved in various biological processes

    Bionic Ring Grooves Design and Experiment of the Suction Cup Applied in Oil-Immersed Substrate

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    The vacuum suction cup is often used as an end effector and widely used in wall-climbing operations. However, there are few vacuum suction cup designs and applications for oil-immersed substrates. Inspired by the surface morphology of the octopus sucker, bionic suction cups with different numbers, diameters, and spacings of the ring grooves were designed. Their normal adsorption force was evaluated on the untreated and polished steel plate in oil. The test results showed that ring grooves positively affected the adsorption force. The bionic suction cup with a groove number of 3, a diameter of 0.5 mm, and a spacing of 3 mm was the most excellent in the test. It achieved normal adsorption forces of 54.83 ± 0.48 N and 43.89 ± 0.69 N on the untreated and polished steel plate. Compared with the standard suction cup, it increased by 32.31% and 12.28% on the untreated and polished steel plate. The regression model between the normal adsorption force and design factors was established based on the adsorption force test results, and the influence law of the ring groove structure parameters on the adsorption force of suction cups on oil-immersed substrates was analyzed. The order of significant effects of groove design parameters on normal adsorption forces was groove diameters, spacings, and numbers. The finite element analysis (FEA) results show that the ring grooves could significantly increase the contact pressure, frictional stress, and sliding distance between the suction cup and the substrate. The ring groove structure effectively improves the adsorption force of the suction cup on the oil-immersed surface by forming a more effective seal and increasing the friction force and adsorption area. This study could provide a reference for developing the actuator of the oil-immersed or lubricated climbing machine

    HEAD: HEtero-Assists Distillation for Heterogeneous Object Detectors

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    Conventional knowledge distillation (KD) methods for object detection mainly concentrate on homogeneous teacher-student detectors. However, the design of a lightweight detector for deployment is often significantly different from a high-capacity detector. Thus, we investigate KD among heterogeneous teacher-student pairs for a wide application. We observe that the core difficulty for heterogeneous KD (hetero-KD) is the significant semantic gap between the backbone features of heterogeneous detectors due to the different optimization manners. Conventional homogeneous KD (homo-KD) methods suffer from such a gap and are hard to directly obtain satisfactory performance for hetero-KD. In this paper, we propose the HEtero-Assists Distillation (HEAD) framework, leveraging heterogeneous detection heads as assistants to guide the optimization of the student detector to reduce this gap. In HEAD, the assistant is an additional detection head with the architecture homogeneous to the teacher head attached to the student backbone. Thus, a hetero-KD is transformed into a homo-KD, allowing efficient knowledge transfer from the teacher to the student. Moreover, we extend HEAD into a Teacher-Free HEAD (TF-HEAD) framework when a well-trained teacher detector is unavailable. Our method has achieved significant improvement compared to current detection KD methods. For example, on the MS-COCO dataset, TF-HEAD helps R18 RetinaNet achieve 33.9 mAP (+2.2), while HEAD further pushes the limit to 36.2 mAP (+4.5).Comment: ECCV 2022, Code: https://github.com/LutingWang/HEA

    Transcriptome Phase Distribution Analysis Reveals Diurnal Regulated Biological Processes and Key Pathways in Rice Flag Leaves and Seedling Leaves

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    Plant diurnal oscillation is a 24-hour period based variation. The correlation between diurnal genes and biological pathways was widely revealed by microarray analysis in different species. Rice (Oryza sativa) is the major food staple for about half of the world's population. The rice flag leaf is essential in providing photosynthates to the grain filling. However, there is still no comprehensive view about the diurnal transcriptome for rice leaves. In this study, we applied rice microarray to monitor the rhythmically expressed genes in rice seedling and flag leaves. We developed a new computational analysis approach and identified 6,266 (10.96%) diurnal probe sets in seedling leaves, 13,773 (24.08%) diurnal probe sets in flag leaves. About 65% of overall transcription factors were identified as flag leaf preferred. In seedling leaves, the peak of phase distribution was from 2:00am to 4:00am, whereas in flag leaves, the peak was from 8:00pm to 2:00am. The diurnal phase distribution analysis of gene ontology (GO) and cis-element enrichment indicated that, some important processes were waken by the light, such as photosynthesis and abiotic stimulus, while some genes related to the nuclear and ribosome involved processes were active mostly during the switch time of light to dark. The starch and sucrose metabolism pathway genes also showed diurnal phase. We conducted comparison analysis between Arabidopsis and rice leaf transcriptome throughout the diurnal cycle. In summary, our analysis approach is feasible for relatively unbiased identification of diurnal transcripts, efficiently detecting some special periodic patterns with non-sinusoidal periodic patterns. Compared to the rice flag leaves, the gene transcription levels of seedling leaves were relatively limited to the diurnal rhythm. Our comprehensive microarray analysis of seedling and flag leaves of rice provided an overview of the rice diurnal transcriptome and indicated some diurnal regulated biological processes and key functional pathways in rice
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