The Synergy of Recombinant Xylanolytic Enzyme on Xylan Hydrolysis

Microbial xylanases or xylanolytic enzyme have received considerable attention over the last years owing to a multitude of  possible applications. These  enzymes  have  potential  in  the  biodegradation of lignocellulosic biomass  to  fuels, chemicals, fruit juice, animal feed and in improving rumen digestion. More recently, the use of xylanases as bleaching agent  in  the  pulp  and  paper  industry  has  been  suggested  to  replace  of some  of  the  chemicals  presently  used  for  this purpose. Such applications could  have an important positive impact on the environment. The purpose of  this research was  determining  the  synergy  of  3 recombinant  xylanolytic  enzymes  (β-xylosidase,  exo-xylanase  and α-L-arabinofuranosidase)  from  recombinant  Eschericia  coli  BL21 (DE-star)  in  xylan  hydrolysis  by  analysis  the  reduction sugar product. Purified of recombinant xylanolytic enzyme β-xylosidase  (Xyl), exo-xylanase  (Exo-Xyl) and α-L-arabinofuranosidase  (Abfa)  with  Ni-NTA resin.  Seven  samples  of  enzyme  (each  and  enzyme  mixture)  used to hydrolyze  xylan    substrate  (oat-spelt  xylan).  Analysis  of  hydrolysis product  was  done  by  HPLC.  The  xylanolytic activities of this enzyme before and after purification were 0,91 and 9,94 U/mL (Exo-Xyl); 1,65 and 14,2 U/mL (Xyl); 0,65  and  5,6  U/mL  (Abfa).  The  xylosidase  activity  were  2,37  and  14,3  U/mL  (Xyl);  1,49  and  10,5  U/mL  (Exo-Xyl); 2,54  and  18,6  U/mL  (Abfa).  The  highest  hydrolysis  product  of  xylan  (xylose)  shown  in  enzyme  mixture  of    exo-xylanase and β-xylosidase was 1,084 mg/mL. &nbsp

Pencirian Produksi Amilase oleh Saccaromyces Cerevisiae W303A Rekombinan

Characterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively