The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-1

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>d at 24 hours post infection and tittered on Vero cells (see Materials & Methods). The error bars shown represent the maximum and minimum complementation ratios obtained from three independent experiments, and the bar height represents the average complementation ratio

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-0

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>cid replacements are indicated with the amino acid position bracketed on the left by the targeted amino acid and on the right by the changed amino acid i.e. Y38A. Carboxyl terminal truncations are indicated by the let (t) following the terminal amino acid of the truncated UL20p. Transmembrane region (TM), Cluster mutant (CL)

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-3

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>Thirty-six hours post-transfection, cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, rabbit anti-FLAG (α FLAG) mAb was used to detect UL20p, mouse anti-V5 (α V5) epitope was used to detect gK, and sheep aTGN46 mAb was used to identify the TGN. First three rows of the confocal pictures show co-localization of UL20p with gK, while rows 4–6 show colocalization of gK with TGN46

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-2

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>K) or gB(syn3) mutation. Viral plaques were visualized by immunohistochemistry at 24 hpi

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-5

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>live conditions with aV5 (gK) mAb for 6 hours. Cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3 × FLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-6

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>cid replacements are indicated with the amino acid position bracketed on the left by the targeted amino acid and on the right by the changed amino acid i.e. Y38A. Carboxyl terminal truncations are indicated by the let (t) following the terminal amino acid of the truncated UL20p. Transmembrane region (TM), Cluster mutant (CL)

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-4

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>oroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3xFLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively