Additional file 3: Figure S3. of Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

TKT and TKTL2 expression in melanoma cells. (A) TKT, (B) TKTL1 and (C) TKTL2 expression in LM-MEL-59 after treatment with two TKTL1 siRNAs or control siRNA for 72 h. Testis tissue was used as positive control for the expression of TKT, TKTL1 and TKTL2. (PPTX 69 kb

Additional file 5: Figure S5. of Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

TKTL1 expression in melanoma affects proliferation of cells. (A) Measurement of absorbance of LM-MEL-59 cells after TKTL1 siRNA or control siRNA treatment for 48 h by performing MTS assay. (B) Measurement of absorbance of LM-MEL-44 cells after overexpression of TKTL1 or empty vector for 48 h. (PPTX 57 kb

Additional file 1: Figure S1. of Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

TKTL1 is expressed in a subset of melanoma tumours and melanoma cell lines. (A) Representative staining patterns for TKTL1 in metastatic melanoma tumors are shown. Arrows indicate nuclear staining for Melanin. Original magnification, 200 Οm. (B) QRT-PCR for expression level of TKTL1 in a panel of 53 metastatic melanoma cell lines. (C) High magnification staining pattern of TKTL1 in melanoma cell line LM-MEL-59. Arrows indicate nuclear staining for Melanin. Original magnification, 200 Οm. (PPTX 1827 kb

Additional file 2: Figure S2. of Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

Correlation of TKTL1 gene expression with DNA methylation in melanoma patients. Exon expression data was compared to methylation patterns of TKTL1 and Spearman correlation coefficients were calculated. Methylation status at the CpG site (A) cg09892236 and (B) cg23106779 was inversely correlated with TKTL1 gene expression in melanoma samples. (PPTX 114 kb