Clinical value of oral fluid chemokines in chronic periodontitis

Activation of cytokine network is one of the key mechanisms in immunopathogenesis of chronic periodontitis (CP), a common dental disease. Therefore, the study of the proteomic (including cytokine) profile of saliva is not coincidentally considered among global research areas in periodontology. However, the existing data on the contents of cytokines / chemokines in oral fluid (OF) in CP are contradictory. This ambiguity of the results can be associated both with a variety of methodological approaches to the cytokine determination, and with different severity of CP in the studied cohorts of patients. Moreover, recent systematic reviews and meta-analyses show that clinical value of chemokines in CP is also not yet determined thus indicating a need for future research in this area.The aim of this study was to assess clinical and diagnostic value of some chemokines from oral fluid in chronic mild periodontitis. The work was based on a study of 18 patients diagnosed with mild chronic periodontitis (MCD-10 K05.3) and 12 practically healthy volunteers with relatively intact periodontal tissue. The diagnosis was based on standard clinical and radiological criteria. In all cases the levels of 8 chemokines were determined using multiparametric fluorescence analysis with magnetic microspheres (xMAP technology, Luminex 200, USA). Statistical analysis was carried out by methods of non-parametric statistics. To determine the predictive value of the test, ROC analysis was performed.It has been shown that CP is accompanied by increased levels of CXCL1 (5.5-fold), CXCL8 (8.1-fold), CXCL12 (3.5-fold), CCL2 (2.7-fold). At the same time, the level of other chemokines did not change significantly. There was a lack of correlations between individual parameters in the group of patients with CP, in contrast to the control group, thus, probably reflecting disturbed mechanisms of “balance” regulation of chemokines. By means of ROC analysis, highly sensitive biomarkers of chronic mild periodontitis were identified. The diagnostic sensitivity and diagnostic specificity were for CXCL1 (91 and 75%, respectively), CXCL8 and CXCL12 (95 and 75%), CCL2 (82 and 75%). The data obtained indicate that the salivary chemokines CXCL1, CXCL8, CXCL10, CXCL12, CCL2 can be considered potential biomarkers of mild chronic periodontitis

Electron transfer in nucleophilic vinyl substitution reactions involving bromovinyl sulfones

It was shown that the reaction of phosphorous hexaalkyltriamides with β-bromovinyl aryl sulfones proceeds via the step of electron transfer with the formation of ion-radicals. © 1982 Plenum Publishing Corporation

Serum nesfatin in overweight and obese children

BACKGROUND: Obesity is one of the most common children diseases. In present time it is being actively studied the natural hormones role in the pathogenesis of obesity. AIMS: To determine the relationship between serum nesfatin level with impaired lipid metabolism in school age children with overweight and obesity. MATERIALS AND METHODS: The study included children with overweight and obesity («case») and healthy children with normal body mass («control») aged 9 to 15 years. All children were determined by concentration in cholesterol blood and its factions, apoA-1, apoB. Enzyme-linked immunosorbent assay (Bio Human Nesfatin Enzyme Immunoassay Kit). RESULTS: Groups of patients with overweight and obesity (n = 53) and healthy children (n = 31) were comparable by age and sex. In obese children, a significant decrease in the level of nesfatin-1 was revealed compared with the group with normal body weight. In cases of children obesity there is a marked decrease in the concentration of nesfatin-1 in serum, especially expressed in the dyslipidemia presence. The nesfatin-1 level are negatively correlated to BMI and body fat content. CONCLUSIONS: The obtained data suggest that along with the known key role of nesfatin in the regulation of eating behavior and appetite, it also affects anthropometric indicators, primarily associated with the accumulation of excess fat mass. Based on the data obtained, nesfatin-1 should be considered as a possible marker for the development of obesity in children

Determination of reference intervals for the cytograms of buccal epithelium

In this article, the study of buccal epithelium in children and adults, both in healthy and with oral pathologies. Karyological and cytoplasmic anomalies of the buccal epithelium were evaluated. Defined reference ranges of indicators buccal cytograms from different age groups. Changes in the indicators in the pathology of the oral cavity were revealedВ данной статье произведено исследование буккального эпителия у детей и взрослых, как у здоровых, так и с патологиями полости рта. Была дана оценка кариологическим и цитоплазматическим аномалиям буккального эпителия. Определены референтные интервалы показателей буккальной цитограммы разных возрастных групп. Выявлены изменения показателей при потологиях полости рта

Inflammation biomarkers at myeloproliferative neoplasms

The article considers the possibility of using biomarkers of inflammation as a potential clinical diagnostic tool in the diagnosis and treatment of myeloproliferative neoplasms. The blood levels of 44 inflammatory biomarkers were determined in the groups, namely: IFN-gamma, IL-12p70, IL-13, IL-1beta, IL-2, IL-4, IL- 5, IL-6, TNF-alpha, GM -CSF, IL-18, IL-10, IL-17A, IL-21, IL-22, IL-23, IL- 27, IL-9, IFN-alpha, IL-31, IL-15, IL-1alpha , IL-1RA, IL-7, TNF-beta, Eotaxin, GRO-alpha, IL-8, IP-10, MCP-1, MIP-1alpha, MIP-1beta, SDF-1alpha, RANTES, NGF-beta, BDNF , EGF, FGF-2, HGF, LIF, PDGF-BB, PIGF-1, SCF, VEGF-A, VEGF-D. An analysis of the data revealed that levels of biomarkers of inflammation in patients diagnosed with true polycythemia and the control group are different. Clonal proliferation of myeloid cells is accompanied by secondary inflammation and abnormal cytokine production. It has been established that patients with myeloproliferative neoplasm are characterized by changes in the cytokine profile, with a predominance of pro-inflammatory cytokines. On the other hand, the increase in anti-inflammatory cytokines is less pronounced.В статье рассматривается возможность использования биомаркеров воспалениякак потенциального клинико – диагностического инструмента при диагностике и лечении миелопролиферативныхновообразований. В группах определялось содержание в крови 44 биомаркеров воспаления, а именно: IFN-gamma, IL-12p70, IL-13, IL-1beta, IL-2, IL-4, IL-5, IL- 6,TNF- alpha, GM-CSF, IL-18, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27, IL-9, IFN-alpha, IL-31, IL-15, IL-1alpha, IL-1RA, IL-7, TNF-beta, Eotaxin, GRO-alpha, IL-8, IP-10, MCP-1, MIP-1alpha, MIP-1beta, SDF-1alpha, RANTES, NGF-beta, BDNF, EGF, FGF-2, HGF, LIF, PDGF-BB, PIGF-1, SCF, VEGF-A, VEGF-D. При анализе данных было выявлено, что уровни биомаркеров воспаления у пациентов с диагнозом истинная полицитемия и контрольной группы отличаются. Клональная пролиферация миелоидных клеток сопровождается вторичным воспалением и патологической выработкой цитокинов. Установлено, что для больных с миелопролиферативным новообразованием характерно изменения в цитокиновом профиле,с преобладанием провоспалительных цитокинов. Сдругойстороны, повышениепротивовоспалительныхцитокиновменеевыражено

Polina Evgenievna Zakharova

Статья посвящена памяти Полины Евгеньевны Захаровой (12.03.1940—28.09.2020), нашего Учителя, звездного астронома, кандидата физико-математических наук, директора Коуровской астрономической обсерватории Уральского университета (1982—2016), вдохновителя и организатора Всероссийских студенческих научных конференций «Физика Космоса», автора учебно-наглядного пособия «Календарь ”Астрономия”», члена ряда профессиональных советов и обществ, человека, беззаветно преданного своему делу — развитию астрономии на Среднем Урале и в России.The article is dedicated to the memory of Polina Evgenievna Zakharova (12.03.1940—28.09.2020), our Teacher, stellar astronomer, Candidate of Science in Physics and Mathematics, director of the Kourovka Astronomical Observatory of the Ural University (1982—2016), inspirer and organizer All-Russian student scientific conferences ”Physics of Space”, the author of the teaching-visual aid Calendar ”Astronomy”, a member of a number of professional councils and societies, a person selflessly devoted to her work — the development of astronomy in the Middle Urals and in Russia

Role of the vascular endothelial growth factor (VEGF) in the immunopathogenesis of chronic opisthorchosis

Described clinic, hemogram, biochemical, immune status in 53 patients with a diagnosis: chronic opisthorchosis. In 47,2% ot patients found to increase the concentration of vascular endothelial growth factor (VEGF). A comparison group of patients, depending on the level of VEGF. Analyzed the relationship with the concentration of VEGF indicators clinics, electrocardiogram, hemogram, biochemistry, phagocytosis, cellular, humoral immunity, cytokines, precursor of brain natriuretic peptide (NtproBNP) and the total antioxidant status. VEGF it should be regarded as a proinflammatory agent.Описана клиника, гемограмма, биохимические показатели, иммунный статус у 53 пациентов с диагнозом: хронический описторхоз. У 47,2% больных установлено повышение концентрации фактора роста эндотелия сосудов (ФРЭС). Проведено сравнение групп пациентов в зависимости от уровня ФРЭС. Проанализированы взаимосвязи концентрации ФРЭС с показателями клиники, электрокардиограммы, гемограммы, биохимии, фагоцитоза, клеточного, гуморального иммунитета, цитокинами, предшественником мозгового натрийуретического пептида и общим антиоксидантным статусом. ФРЭС следует рассматривать как провоспали-тельный агент

Study of the effectiveness of a combination of laser curettage and injections of hyaluronic acid-based gel (experiment on laboratory animals)

Study of the effectiveness of a combination of laser curettage and injections of hyaluronic acid-based gel (experiment on laboratory animals