A shadowgraph study of two proposed Shuttle-C launch vehicle configurations

A shadowgraph study concerning two of the proposed Shuttle-C launch vehicle configurations are presented. These shadowgraphs were obtained from a wind tunnel test performed in Marshall Space Flight Center's 14-in trisonic wind tunnel at various angles-of-attack and roll angles over the Mach range of 0.6 to 4.96. Variations in payload bay length were also evaluated. Major flow field phenomena can easily be seen in the shadowgraphs. Shadowgraphs are a valuable resource. They are used in the analysis of the external flow conditions the launch vehicle encounters through the ascent stage of flight. Subsequent reports will contain shadowgraph studies for other launch vehicle configurations also tested in the Marshall Space Flight Center's 14-in trisonic wind tunnel

Aerodynamic characteristics of the National Launch System (NLS) 1 1/2 stage launch vehicle

The National Aeronautics and Space Administration (NASA) is studying ways of assuring more reliable and cost effective means to space. One launch system studied was the NLS which included the l l/2 stage vehicle. This document encompasses the aerodynamic characteristics of the 1 l/2 stage vehicle. To support the detailed configuration definition two wind tunnel tests were conducted in the NASA Marshall Space Flight Center's 14x14-Inch Trisonic Wind Tunnel during 1992. The tests were a static stability and a pressure test, each utilizing 0.004 scale models. The static stability test resulted in the forces and moments acting on the vehicle. The aerodynamics for the reference configuration with and without feedlines and an evaluation of three proposed engine shroud configurations were also determined. The pressure test resulted in pressure distributions over the reference vehicle with and without feedlines including the reference engine shrouds. These pressure distributions were integrated and balanced to the static stability coefficients resulting in distributed aerodynamic loads on the vehicle. The wind tunnel tests covered a Mach range of 0.60 to 4.96. These ascent flight aerodynamic characteristics provide the basis for trajectory and performance analysis, loads determination, and guidance and control evaluation

Stereo-PIV measurements of spatio-temporal turbulence correlations in an axisymmetric jet

Stereoscopic three-component particle image velocimetry (3C-PIV) measurements have been made in a turbulent round jet to investigate the spatio-temporal correlations that are the origin of aerodynamic noise. Restricting attention to subsonic, isothermal jets, measurements were taken in a water flow experiment where, for the same Reynolds number and nozzle size, the shortest time scale of the dynamically important turbulent structures is more than an order of magnitude greater that in equivalent airflow experiments, greatly facilitating time-resolved PIV measurements. Results obtained (for a jet nozzle diameter and velocity of 40 mm and 1 m s\u1000001, giving Re D 4 104) show that, on the basis of both single-point statistics and two-point quantities (correlation functions, integral length scales) the present incompressible flow data are in excellent agreement with published compressible, subsonic airflow measurements. The 3C-PIV data are first compared to higher-spatial-resolution 2C-PIV data and observed to be in good agreement, although some deterioration in quality for higher-order correlations caused by high-frequency noise in the 3C-PIV data is noted. A filter method to correct for this is proposed, based on proper orthogonal decomposition (POD) of the 3C-PIV data. The corrected data are then used to construct correlation maps at the second- and fourth-order level for all velocity components. The present data are in accordance with existing hot-wire measurements, but provide significantly more detailed information on correlation components than has previously been available. The measured relative magnitudes of various components of the two-point fourth-order turbulence correlation coefficient (Rij;kl) – the fundamental building block for free shear flow aerodynamic noise sources – are presented and represent a valuable source of validation data for acoustic source modelling. The relationship between fourth-order and second-order velocity correlations is also examined, based on an assumption of a quasi-Gaussian nearly normal p.d.f. for the velocity fluctuations. The present results indicate that this approximation shows reasonable agreement for the measured relative magnitudes of several correlation components; however, areas of discrepancy are identified, indicating the need for work on alternative models such as the shell turbulence concept of Afsar (Eur. J. Mech. (B/Fluids), vol. 31, 2012, pp. 129–139)

Computational Study of Aero-acoustic Sources in Perforate Silencers

Reynolds Averaged Navier Stokes and Large Eddy Simulations of two perforate plates at a overall pressure ratio of 1.45 have been performed to allow analysis of the sensitivity of acoustic noise sources to porosity. Two geometries are presented: A 23% porosity and a 40% porosity 1mm plate with 2mm diameter holes. Results presented in this paper show the initial jetlet and fully merged jet flow-field to be sensitive to the porosity and the presence of partial holes around the circumference of the plate. The increase in porosity reduces the available entrainment flow, and increases the local jetlet interaction and resultant turbulence levels. This interaction fundamentally changes the flow structure from coherent vortex rings (found at low porosity) to a helical structure. The 2nd and 4th order spatio- temporal correlation Rij and Rij,kl are presented as suggested validation data for acoustic source modeling together with far-field noise spectra obtained via a Ffowcs-Williams & Hawkings surface integral method

Variability Measures of Positive Random Variables

During the stationary part of neuronal spiking response, the stimulus can be encoded in the firing rate, but also in the statistical structure of the interspike intervals. We propose and discuss two information-based measures of statistical dispersion of the interspike interval distribution, the entropy-based dispersion and Fisher information-based dispersion. The measures are compared with the frequently used concept of standard deviation. It is shown, that standard deviation is not well suited to quantify some aspects of dispersion that are often expected intuitively, such as the degree of randomness. The proposed dispersion measures are not entirely independent, although each describes the interspike intervals from a different point of view. The new methods are applied to common models of neuronal firing and to both simulated and experimental data

NPM1 Deletion Is Associated with Gross Chromosomal Rearrangements in Leukemia

BACKGROUND: NPM1 gene at chromosome 5q35 is involved in recurrent translocations in leukemia and lymphoma. It also undergoes mutations in 60% of adult acute myeloid leukemia (AML) cases with normal karyotype. The incidence and significance of NPM1 deletion in human leukemia have not been elucidated. METHODOLOGY AND PRINCIPAL FINDINGS: Bone marrow samples from 145 patients with myelodysplastic syndromes (MDS) and AML were included in this study. Cytogenetically 43 cases had isolated 5q-, 84 cases had 5q- plus other changes and 18 cases had complex karyotype without 5q deletion. FISH and direct sequencing investigated the NPM1 gene. NPM1 deletion was an uncommon event in the "5q- syndrome" but occurred in over 40% of cases with high risk MDS/AML with complex karyotypes and 5q loss. It originated from large 5q chromosome deletions. Simultaneous exon 12 mutations were never found. NPM1 gene status was related to the pattern of complex cytogenetic aberrations. NPM1 haploinsufficiency was significantly associated with monosomies (p<0.001) and gross chromosomal rearrangements, i.e., markers, rings, and double minutes (p<0.001), while NPM1 disomy was associated with structural changes (p=0.013). Interestingly, in complex karyotypes with 5q- TP53 deletion and/or mutations are not specifically associated with NPM1 deletion. CONCLUSIONS AND SIGNIFICANCE: NPM1/5q35 deletion is a consistent event in MDS/AML with a 5q-/-5 in complex karyotypes. NPM1 deletion and NPM1 exon 12 mutations appear to be mutually exclusive and are associated with two distinct cytogenetic subsets of MDS and AML

Chronic Granulomatous Disease; fundamental stages in our understanding of CGD

It has been 50 years since chronic granulomatous disease was first reported as a disease which fatally affected the ability of children to survive infections. Various milestone discoveries from the insufficient ability of patients' leucocytes to destroy microbial particles to the underlying genetic predispositions through which the disease is inherited have had important consequences. Longterm antibiotic prophylaxis has helped to fight infections associated with chronic granulomatous disease while the steady progress in bone marrow transplantation and the prospect of gene therapy are hailed as long awaited permanent treatment options. This review unearths the important findings by scientists that have led to our current understanding of the disease

Intermittent Hypoxia-Induced Cognitive Deficits Are Mediated by NADPH Oxidase Activity in a Murine Model of Sleep Apnea

Background: In rodents, exposure to intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), is associated with neurobehavioral impairments, increased apoptosis in the hippocampus and cortex, as well as increased oxidant stress and inflammation. Excessive NADPH oxidase activity may play a role in IH-induced CNS dysfunction. Methods and Findings: The effect of IH during light period on two forms of spatial learning in the water maze and well as markers of oxidative stress was assessed in mice lacking NADPH oxidase activity (gp91phox _/Y) and wild-type littermates. On a standard place training task, gp91phox _/Y displayed normal learning, and were protected from the spatial learning deficits observed in wild-type littermates exposed to IH. Moreover, anxiety levels were increased in wild-type mice exposed to IH as compared to room air (RA) controls, while no changes emerged in gp91phox _/Y mice. Additionally, wild-type mice, but not gp91phox _/Y mice had significantly elevated levels of NADPH oxidase expression and activity, as well as MDA and 8-OHDG in cortical and hippocampal lysates following IH exposures. Conclusions: The oxidative stress responses and neurobehavioral impairments induced by IH during sleep are mediated, at least in part, by excessive NADPH oxidase activity, and thus pharmacological agents targeting NADPH oxidase may provid

Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes

Copy number variants (CNVs) are major contributors to genomic imbalance disorders. Phenotyping of 137 unrelated deletion and reciprocal duplication carriers of the distal 16p11.2 220 kb BP2-BP3 interval showed that these rearrangements are associated with autism spectrum disorders and mirror phenotypes of obesity/underweight and macrocephaly/microcephaly. Such phenotypes were previously associated with rearrangements of the non-overlapping proximal 16p11.2 600 kb BP4-BP5 interval. These two CNV-prone regions at 16p11.2 are reciprocally engaged in complex chromatin looping, as successfully confirmed by 4C-seq, fluorescence in situ hybridization and Hi-C, as well as coordinated expression and regulation of encompassed genes. We observed that genes differentially expressed in 16p11.2 BP4-BP5 CNV carriers are concomitantly modified in their chromatin interactions, suggesting that disruption of chromatin interplays could participate in the observed phenotypes. We also identified cis- and trans-acting chromatin contacts to other genomic regions previously associated with analogous phenotypes. For example, we uncovered that individuals with reciprocal rearrangements of the trans-contacted 2p15 locus similarly display mirror phenotypes on head circumference and weight. Our results indicate that chromosomal contacts’ maps could uncover functionally and clinically related genes.Molecular Psychiatry advance online publication, 31 May 2016; doi:10.1038/mp.2016.84