Alcohol, assault and licensed premises in inner-city areas

This report contains eight linked feasibility studies conducted in Cairns during 2010. These exploratory studies examine the complex challenges of compiling and sharing information about incidents of person-to-person violence in a late night entertainment precinct (LNEP). The challenges were methodological as well as logistical and ethical. The studies look at how information can be usefully shared, while preserving the confidentiality of those involved. They also examine how information can be compiled from routinely collected sources with little or no additional resources, and then shared by the agencies that are providing and using the information.Although the studies are linked, they are also stand-alone and so can be published in peer-reviewed literature. Some have already been published, or are ‘in press’ or have been submitted for review. Others require the NDLERF board’s permission to be published as they include data related more directly to policing, or they include information provided by police.The studies are incorporated into the document under section headings. In each section, they are introduced and then presented in their final draft form. The final published form of each paper, however, is likely to be different from the draft because of journal and reviewer requirements. The content, results and implications of each study are discussed in summaries included in each section.Funded by the National Drug Law Enforcement Research Fund, an initiative of the National Drug StrategyAlan R Clough (PhD) School of Public Health, Tropical Medicine and Rehabilitation Sciences James Cook UniversityCharmaine S Hayes-Jonkers (BPsy, BSocSci (Hon1)) James Cook University, Cairns.Edward S Pointing (BPsych) James Cook University, Cairns

Diverse hypolithic refuge communities in the McMurdo Dry Valleys

Hyper-arid deserts present extreme challenges to life. The environmental buffering provided by quartz and other translucent rocks allows hypolithic microbial communities to develop on sub-soil surfaces of such rocks. These refuge communities have been reported, for many locations worldwide, to be predominantly cyanobacterial in nature. Here we report the discovery in Antarctica’s hyper-arid McMurdo Dry Valleys of three clearly distinguishable types of hypolithic community. Based on gross colonization morphology and identification of dominant taxa, we have classified hypolithic communities as Type I (cyanobacterial dominated), Type II (fungal dominated) and Type III (moss dominated). This discovery supports a growing awareness of the high biocomplexity in Antarctic deserts, emphasizes the possible importance of cryptic microbial communities in nutrient cycling and provides evidence for possible successional community processes within a cold arid landscape

Molecular and morphological characterization of free-floating filamentous cyanobacterial mats from geothermal springs in the Philippines

A novel cyanobacterial mat type is characterized from near-neutral pH, low sulphide geothermal springs of 45-60 °C in the Philippines. Mats were free floating, several metres in diameter and several cm in thickness. The upper surface of mats was covered in a waxy scytonemin-like layer, solvent extracts of which absorbed light strongly at 384nm. Light microscopy revealed mats to posses highly ordered layers of air spaces at both the macroscopic and microscopic level, apparently as an adaptation to buoyancy. Morphospecies composition was exclusively filamentous, with Fischerella-like and Oscillatoria-like taxa closely associated throughout mats. Abundant heterocystous cells were observed in Fischerella filaments, suggesting nitrogen fixation occurs in these mats. Morphological structure did not vary among mats from pools of different temperature, but several 16S rDNA-defined genotypes were resolved by DGGE with some displaying greater thermophily than others. Sequencing of fourteen DGGE bands (Genbank accession numbers: AY236467-AY236480) yielded nine novel Fischerella sequences, whilst the five Oscillatoria sequences showed high similarity to other thermophilic Oscillatoria sequences. These data are relevant to astrobiology in that they expand our knowledge of oxygenic photosynthetic community diversity in geothermal environments, which serve as modern analogues for early life on Earth and other planets. Acknowledgements The authors are extremely grateful to the Philippine Institute of Volcanology and Seismology (PHIVOLCS) for advice and assistance with fieldwork. This work was supported by grants awarded by The University of Hong Kong CRCG Seed Funding for Basic Research and Small Projects programmes.published_or_final_versio

How can we use realist evaluation to better inform research into alcohol-related assaults in night-time economies? A case study using CCTV

The problem of alcohol-related assaults (ARAs) in night-time economy (NTE) precincts is an ongoing issue of policy concern and research interest. One punch truly can kill. This thesis examines the feasibility of a multi-agency linking method to more accurately count these kinds of assaults in close to real-time in order to inform suitable responses by Queensland liquor accords. This can provide more timely evidence to evaluate interventions. This thesis was the first Australian research to achieve this. The Cairns Hospital Emergency Department, the Queensland Police Service and the Cairns Regional Council Closed Circuit Television (CCTV) system are capable of providing relevant data regularly. Venues can provide relevant data but capacity to do so regularly is limited. Results support previous research regarding prevalence and time of occurrence of NTE ARAs. The thesis also uses Realist Evaluation to examine how an urban, open-space CCTV system responds to, and reduces, these NTE ARAs. The realist evaluation approach explains why quasi-experimental studies have found CCTV has no effect on this type of offence, but practitioners see CCTV as vital. The thesis shows why the theory of deterrence through CCTV does not apply to NTE ARAs. Conflicting empirical results regarding the efficacy of CCTV in broader crime reduction are explained. A case study analysis of CCTV to address NTE ARAs identifies initial context-mechanism-outcome configurations. The thesis provides evidence that suggests realist evaluation is an appropriate approach to address ongoing methodological problems in studying ways to reduce NTE ARAs

Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture

The white-rot fungus Pycnoporus sanguineus produces laccase under a range of C/N ratios in submerged liquid culture. Enzyme production was increased 50 fold in the presence of 20 μM xylidine to a maximum of 1368 U L-1 in a high carbon low nitrogen medium. Slight repression of enzyme production was observed in high nitrogen culture medium. Other potential inducers were less effective (Tween 80, wood fibres) and reduced the stimulation observed by xylidine alone when included in the same culture medium. Veratryl alcohol failed to stimulate laccase production. Activity of the enzyme activity in crude culture nitrate was stable at temperatures of 35 C and below with a pH optimum of 3.0. The laccase of P. sanguineus was identified as a ca 65 kDa protein produced as multiple isoforms.published_or_final_versio

Identification and characterization of thermophilic Synechococcus spp. isolates from Asian geothermal springs

Two thermophilic cyanobacterial strains, Ts and Bs, collected from Asian geothermal springs were identified morphologically and phylogenetically as Synechococcus in the order Chroococcales and were isolated into axenic cultures. In addition to the high similarities between their full 16S rRNA gene sequences, both strains also shared similar pigment profiles and fatty acid compositions but with varied ratios. Strain Ts had elevated levels of photoprotective pigments such as carotenoid and scytonemin even after prolonged culture under identical laboratory conditions, whereas strain Bs produced more chlorophyll a per unit cell volume, perhaps resulting from UV adaptation in the natural habitats. In addition, strain Ts had more content than strain Bs in terms of the total fatty acids and the proportion of unsaturated fatty acids. Neither isolate was able to fix nitrogen, and they had zero susceptibility to ampicillin and streptomycin. © 2007 NRC.published_or_final_versio

Airborne bacterial populations above desert soils of the McMurdo Dry Valleys, Antarctica

Bacteria are assumed to disperse widely via aerosolized transport due to their small size and resilience. The question of microbial endemicity in isolated populations is directly related to the level of airborne exogenous inputs, yet this has proven hard to identify. The ice-free terrestrial ecosystem of Antarctica, a geographically and climatically isolated continent, was used to interrogate microbial bio-aerosols in relation to the surrounding ecology and climate. High-throughput sequencing of bacterial ribosomal RNA (rRNA) genes was combined with analyses of climate patterns during an austral summer. In general terms, the aerosols were dominated by Firmicutes, whereas surrounding soils supported Actinobacteria-dominated communities. The most abundant taxa were also common to aerosols from other continents, suggesting that a distinct bio-aerosol community is widely dispersed. No evidence for significant marine input to bio-aerosols was found at this maritime valley site, instead local influence was largely from nearby volcanic sources. Back trajectory analysis revealed transport of incoming regional air masses across the Antarctic Plateau, and this is envisaged as a strong selective force. It is postulated that local soil microbial dispersal occurs largely via stochastic mobilization of mineral soil particulates

Comparison of DNA and RNA, and Cultivation Approaches for the Recovery of Terrestrial and Aquatic Fungi from Environmental Samples

Estimates of fungal biodiversity from environmental samples are all subject to bias. Major issues are that the commonly adopted cultivation-based approaches are suitable for taxa which grow readily under laboratory conditions, while the DNA-based approaches provide more reliable estimates, but do not indicate whether taxa are metabolically active. In this study, we have evaluated these approaches to estimate the fungal diversity in soil and freshwater samples from a subtropical forest, and compared these to RNA-based culture-independent approach intended to indicate the metabolically active fungal assemblage. In both soil and freshwater samples, the dominant taxon recovered by all three approaches was the same (Anguillospora furtiva). This taxon was cultivable from all samples and comprised 85–86 % DNA libraries and 90–91 % RNA libraries. The remaining taxa were phylogenetically diverse and spanned the Ascomycota, Basidiomycota, and Fungi incertae sedis. Their recovery was not consistent among the three approaches used and suggests that less abundant members of the assemblage may be subjected to greater bias when diversity estimates employ a single approach. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00284-012-0256-7) contains supplementary material, which is available to authorized users