Evaluation of the stability of 3D-printed paracetamol tablets stored at different relative humidities

Abstract of the communication presented at the Controlled Release Society Virtual Annual Meeting, 29 June-2 July 2020N/

Co-formability, solubility enhancement and stability of Olanzapine co-amorphous systems produced with different co-formers

Abstract of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/

Characterization and stability of co-amorphous systems containing Olanzapine and sulfonic acids

Abstract of the communication presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/

Comparison of the co-amorphization ability of Olanzapine with amino, carboxylic and sulfonic acids

Abstract of the poster presented at the 3rd European Conference on Pharmaceutics. 25-26 March 2019, Bologne, ItalyN/

3D-Printing of paracetamol tablets by fused deposition modelling

Abstract of poster presented at the 12th PBP World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Wien, Austria, 11-14 May 2021 (virtual Meeting)N/

Study of the stability of acetaminophen extrudates for 3D printing prepared by hot melt extrusion when stored at different relative humidites

Poster presented at the PSWC2020 Virtual (7th FIP Pharmaceutical Sciences World Congress), 4-6 October 2020N/

Biodegradation of mono-, di- and trifluoroacetate by microbial cultures with different origins

This work focused on the biodegradation of three structurally related fluoroacetates (FAs), mono- (MFA), di- (DFA) and trifluoroacetate (TFA), using as microbial inocula samples collected from a site with a long history of industrial contamination and activated sludge obtained from a municipal wastewater treatment plant. Biodegradation experiments were carried out under different modes of substrate supplementation, which included (i) FAs fed as sole carbon sources; (ii) FAs (only for DFA and TFA) fed in co-metabolism with sodium acetate; and (iii) mixtures of MFA with DFA or TFA. Biodegradation of the target compounds was assessed through fluoride ion release. Defluorination was obtained in the cultures fed with MFA, while DFA and TFA were recalcitrant in all tested conditions. When present in mixture, DFA was shown to inhibit biodegradation of MFA, while TFA had no effect. A total of 13 bacterial isolates obtained from MFA degrading cultures were found to degrade 20mgL-1 of this compound, as single strains, when supplemented as a sole carbon source. Sequencing of the 16S rRNA gene indicated that among these degrading bacteria only Delftia acidovorans had been previously reported to be able to degrade MFA. This work shows that, despite their similar chemical structures, biodegradation of the three tested FAs is very distinct and draws attention to the unknown impacts that the accumulation of DFA and TFA may have in the environment as a result of their high recalcitrance.info:eu-repo/semantics/publishedVersio

Rapid optimization of chromatography operating conditions using a nano- liter scale column on a microfluidic chip with integrated pneumatic valves and optical sensors

Purification of monoclonal antibodies (mAbs) is traditionally achieved by chromatographic separations, which are very robust but require time-consuming optimization on a case-by-case, particularly if a non-affinity step is used. In this context, multimodal chromatography has been explored as a versatile and cost-effective alternative to the established affinity step employed for capturing mAbs. However, selective capture/polishing of a target mAb using such multimodal ligands comes with the need for extensive and time-consuming optimization, due to the multitude of interactions that can be simultaneously promoted in the ligand. In this work, we developed a novel microfluidic platform comprising multimodal chromatography beads inside micro-columns for rapid screening of operating conditions. Sequential liquid insertion in the device was achieved by using integrated pneumatic valves and the chromatographic assays were combined with a signal acquisition module for on-chip fluorescence measurements. Please click Additional Files below to see the full abstract

Analytical affinity chromatography-on-a-chip for selective capture and sensitive detection of protein and polynucleotide biomarkers

Affinity Chromatography is a powerful technique which has been applied to the highly selective purification of several biomolecules from complex mixtures. This technique is currently a core technology in the industrial purification of disruptive biopharmaceuticals such as monoclonal antibodies. The use of high affinity ligands, together with densely functionalized three-dimensional solid-phase supports, confers a remarkable analytical potential, making it a current standard for the quantification of several compounds in certified laboratories, ranging from health biomarkers to environmental contaminants. Aiming at extending the application of affinity chromatography to a portable setup, we report the miniaturization of this system down to nL-scale, by trapping Q-sepharose or protein-A agarose beads in microfluidic channels with total volumes ranging from 60 to 210 nL. This versatile and simple platform combined the high surface area and robust surface chemistry provided by the chromatographic media with the high degree of fluidic control, portability, improved reaction kinetics and low reagent expenditure inherent to microfluidics. Furthermore, the microfluidic structures are simple in terms of microfabrication and can be sequentially operated using standard pipette tips and a negative pressure source at the outlet (Figure A). This system was tested within the scope of prostate cancer diagnostics for the capture of protein and polynucleotide biomarkers. Along these lines, prostate specific antigen (PSA) was selectively captured from unprocessed human serum and a 23 bp polynucleotide (ssDNA analogous to micro RNA MIR145) in fetal bovine serum as model matrix, by coupling a monoclonal anti-PSA IgG2a with protein-A beads or a complementary ssDNA strand with Q-sepharose beads, respectively. The assay schematics are described in Figure A. Clinically relevant sensitivities below 10 ng/mL PSA (Figure B) and 10 pM polynucleotide were achieved using a horseradish peroxidase-labelled reporter and measuring chemiluminescence directly on the bead surface. The results demonstrate a high potential for the miniaturization of analytical affinity chromatography, providing good sensitivities in a portable setup, particularly considering the amenability of integrating miniaturized thin-film sensors for optical transduction, as previously demonstrated by our group. Please click Additional Files below to see the full abstract