(A–C) After CNAP, the final concentrated eluates were resolved in the first dimension by 6–16% BN-PAGE and in the second dimension by 12–18% SDS-PAGE

SYPRO Ruby detected bands were extracted, digested with trypsin, and proteins identified by LC–MS/MS. Respiratory complex III and IV subunits are labeled in red and blue, respectively, and members of the mitochondrial carrier family in purple. The pink circle highlights contaminating catalase (verified by LC–MS/MS) from the BN-PAGE molecular weight markers. (D–F) After CNAP, the final concentrated eluates were resolved by 2D BN/SDS-PAGE and immunoblotted for the indicated mitochondrial proteins. = 3. The sources of the purified extracts were (A and D) Δ[AAC2], (B and E) Δ[CNAPAAC2], and (C and F) ΔΔ[CNAPAAC2].<p><b>Copyright information:</b></p><p>Taken from "Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane"</p><p></p><p>The Journal of Cell Biology 2008;182(5):937-950.</p><p>Published online 8 Sep 2008</p><p>PMCID:PMC2528576.</p><p></p